32 results about "Boar sperm" patented technology

The invention relates to a preparation method of an agonist for improving stock boar sperm activity. The method includes the steps: (1) taking out a boar pig testicular tissue to soak the tissue; (2)cleaning the testicular tissue by PBS buffer solution; (3) adding cell culture fluid to culture the tissue, and centrifuging mixture to remove supernatant; (4) adding hyaluronidase and collagenase IVto vibrate mixture, adding cell culture fluid, and centrifuging mixture to remove supernatant; (5) adding trypsin and deoxyribonuclease to vibrate mixture, and adding cell culture fluid; (6) filter mixture by a cell screen; (7) centrifuging mixture to remove supernatant, and adding cell culture fluid; (8) performing cell culture passage; (9) culturing cell fluid, and storing the cell fluid to obtain the agonist. The agonist has the advantages that the agonist is good in application effect, low in cost and easy to produce.

The invention discloses a boar feed. The boar feed contains corns, bean pulp, fish meal, wheat germ, extruded soybeans, calcium hydrophosphate, mountain flour, salt, 50% choline chloride, a micro-ecological preparation, lysine, methionine, threonine, and 0.5-1 part of a boar premix. Per kilogram of the boar premix contains 3500-12000IU of vitamin A, 750-4500IU of vitamin D, 20-100IU of vitamin E, 4-35 mg of copper, 50-250 mg of iron, 40-150 mg of zinc, 16-80 mg of manganese, 0.2-0.8 g of L-carnitine, 5-15 g of omega 3 fish oil, 0.1-0.3 g of selenium yeast, and 0.5-2 g of medium-chain fatty acid MCT. VA, VE, carnitine, omega 3 fish oil and medium-chain fatty acid MCT are added into the boar feed disclosed by the invention; furthermore, the micro-ecological preparation in the invention is used in a matched manner; therefore, the boar sperm density and the total sperm number are increased; the sperm deformation rate of breeding boars is obviously reduced; and thus, the breeding performance of boars is greatly improved.

The invention discloses a method for improving the integrity of cytoskeletons after freeze thawing of preserved boar sperm. The method comprises the following steps of: collecting the boar sperm from an adult, healthy and carnal exuberant boar; extracting low density lipoprotein (LDL) from pigeon eggs; and freezing the boar sperm by using the LDL as a freezing protective agent. By adopting the method for improving the integrity of the cytoskeletons after freeze thawing of the preserved boar sperm, 9 percent LDL serving as the freezing protective agent is added, so that the integrity of the cytoskeletons after freeze thawing of the boar sperm is improved, the expression quantity of actin in the cytoskeletons of the boar sperm can be obviously improved, meanwhile, the expression quantity of tubulin is closer to that of fresh sperm, the survival rate, vitality and fertilization rate of the freeze-thawed boar sperm are effectively improved, the requirements of real use are furthest met, and the method has relatively strong popularization and application values.

The invention relates to a feed for promoting estrus of boars, which belongs to the technical field of production of feeds for pigs and is used for promoting the estrus of boars and improving the boar sperm quality. The feed for promoting the estrus of the boars and improving the boar sperm quality can be used for improving the reproduction performance of boars of child-bearing age. The feed for promoting the estrus of the boars consists of a conventional feed and Chinese herbal medicines and is characterized in that the conventional feed consists of a premix feed consisting of corns, bean pulp, imported fish meal, corn gluten meal, bran, wheat middling, soybean oil, calcium hydrophosphate, mountain flour, salt, lysine, choline chloride and composite vitamins for boars, a composite microelement premix feed and cysteamine; the Chinese herbal medicines include fenugreek grass, actinolite, prepared rehmannia root, cynomorium songaricum and longspur epimedium. According to the feed, on the basis of an optimal formula, the medicine property of the Chinese herbal medicines is sufficiently utilized, the reproduction organism functions of the boars are improved, the estrus of the boars is promoted, the sperm quality of the boars and the reproduction performance of the boars of child-bearing age are improved and the economic benefit is increased.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A / G and G / A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention belongs to the technical field of pig molecular marker screening, and in particular relates to a molecular marker associated with boar sperm concentration traits. The molecular marker is cloned from a gene fragment with accession number WU_10.2_17_4574599, and the gene is typed by gene chip technology , screened to obtain a molecular marker associated with pig sperm concentration traits, its nucleotide sequence is shown in SEQ ID NO: 1, there is a T / C allelic mutation at the 101st base of the sequence , when the 101st nucleotide of SEQ ID NO: 1 is C, it is determined that the pig has a higher sperm concentration. The invention provides a new SNP molecular marker resource for marker-assisted selection of pig semen traits.

The invention belongs to the technical field of screening of molecular markers of boars, and particularly relates to a molecular marker relative to sperm viability and a total sperm number of a boar and application. The molecular marker is cloned from a gene segment with a login number of ALGA 0001958. Through a gene chip technology, a gene is typed, screening is conducted to obtain the molecularmarker relative to characters of the sperm viability and the total sperm number of the boar, a marked nucleotide sequence is as shown in SEQ ID NO:1, A / G allele mutation exists in a base position of asite 101 of the sequence, and when nucleotide of the site 101 on the nucleotide sequence as shown in the SEQ ID NO:1 is A, the fact that the boar has higher sperm viability and a larger total sperm number is judged. The invention discloses a screening method of a molecular marker relative to a boar seminal fluid character and correlation analysis thereof. New SNP marker resources are provided formarker assisted selection of the characters of the sperm viability and the total sperm number of the boars.

The invention discloses a mixed feed for improving antioxidant activity of the sperm of a boar. The mixed feed is characterized by comprising the following raw materials by weight: 40 to 60% of corn, 5 to 20% of fish meal, 5 to 20% of wheat, 10 to 25% of wheat bran, 0.2 to 5% of a soybean small peptide additive, 0.05 to 2% of taurine, 2 to 10% of soybean meal, 2 to 8% of plant oil, 1 to 2% of a calcium element additive, 0.1 to 0.8% of salt, 0.05 to 0.5% of N-carbamylglutamic acid, 0.1 to 0.3% of a composite trace element additive, 0.03 to 0.08% of a composite vitamin additive, 0.005 to 1% of anthocyanidin, 0.05 to 2% of tea polyphenol, 0.5 to 1.5% of amino acid and 0 to 5% of bentonite. The mixed feed is applicable to the boar, can effectively increase the amount of boar seminal fluid, improve sperm vitality and sperm density, reduce the rate of sperm deformation and enhance antioxidant activity of the sperm of the boar and is of great significance to improvement of the quality of boar seminal fluid.