32 results about "Boar sperm" patented technology

The invention belongs to the technical field of livestock molecular marker preparation, and concretely relates to a molecular marker related to a sperm activity character of a boar and application. The molecular marker is screened from a PRM2 gene; a nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. An allelic mutation R exists in nucleotide in position 169 of a sequence table, R is G or A, and the mutation causes the polymorphism of NaeI-RFLP. The invention further discloses a primer pair for amplifying PRM2 gene SNP (Single Nucleotide Polymorphism), and the primer pair is also the primer pair for detecting the screened molecular marker provided by the invention. The invention builds a method for detecting the polymorphisms of the sperm activity character of the boar. Association analytic application shows that the molecular marker provided by the invention can be used as the marker for assistant selection of the sperm activity character of the boar.

The invention relates to a feed for promoting estrus of boars, which belongs to the technical field of production of feeds for pigs and is used for promoting the estrus of boars and improving the boar sperm quality. The feed for promoting the estrus of the boars and improving the boar sperm quality can be used for improving the reproduction performance of boars of child-bearing age. The feed for promoting the estrus of the boars consists of a conventional feed and Chinese herbal medicines and is characterized in that the conventional feed consists of a premix feed consisting of corns, bean pulp, imported fish meal, corn gluten meal, bran, wheat middling, soybean oil, calcium hydrophosphate, mountain flour, salt, lysine, choline chloride and composite vitamins for boars, a composite microelement premix feed and cysteamine; the Chinese herbal medicines include fenugreek grass, actinolite, prepared rehmannia root, cynomorium songaricum and longspur epimedium. According to the feed, on the basis of an optimal formula, the medicine property of the Chinese herbal medicines is sufficiently utilized, the reproduction organism functions of the boars are improved, the estrus of the boars is promoted, the sperm quality of the boars and the reproduction performance of the boars of child-bearing age are improved and the economic benefit is increased.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A / G and G / A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention discloses a mixed feed for improving antioxidant activity of the sperm of a boar. The mixed feed is characterized by comprising the following raw materials by weight: 40 to 60% of corn, 5 to 20% of fish meal, 5 to 20% of wheat, 10 to 25% of wheat bran, 0.2 to 5% of a soybean small peptide additive, 0.05 to 2% of taurine, 2 to 10% of soybean meal, 2 to 8% of plant oil, 1 to 2% of a calcium element additive, 0.1 to 0.8% of salt, 0.05 to 0.5% of N-carbamylglutamic acid, 0.1 to 0.3% of a composite trace element additive, 0.03 to 0.08% of a composite vitamin additive, 0.005 to 1% of anthocyanidin, 0.05 to 2% of tea polyphenol, 0.5 to 1.5% of amino acid and 0 to 5% of bentonite. The mixed feed is applicable to the boar, can effectively increase the amount of boar seminal fluid, improve sperm vitality and sperm density, reduce the rate of sperm deformation and enhance antioxidant activity of the sperm of the boar and is of great significance to improvement of the quality of boar seminal fluid.

The invention provides a two-layer percoll density gradient centrifugal separation method of boar sperms. The method is suitable for normal sperm specimen and sperm specimen with low sperm density or low sperm motility, and is a simple, convenient and fast sperm treatment method. The method can be used for remove leucocytes, bacteria, fragments and anti-sperm antibodies in the sperms and improving the delayed sperm liquefaction, the sperm in-vitro capacitation and the like, so that the sperms can effectively fertilize ova to improve the pregnancy rate, and the percentage of the sperms with motility and normal shape in the obtained sperms is about 95%. The method provided by the invention is suitable for screening sperms with motility from fresh boar sperms and diluted sperms, thus providing high-quality sperms for the study of boar sperm capacitation mechanism and in-vitro fertilization.

The invention discloses a boar feed. The boar feed contains corns, bean pulp, fish meal, wheat germ, extruded soybeans, calcium hydrophosphate, mountain flour, salt, 50% choline chloride, a micro-ecological preparation, lysine, methionine, threonine, and 0.5-1 part of a boar premix. Per kilogram of the boar premix contains 3500-12000IU of vitamin A, 750-4500IU of vitamin D, 20-100IU of vitamin E, 4-35 mg of copper, 50-250 mg of iron, 40-150 mg of zinc, 16-80 mg of manganese, 0.2-0.8 g of L-carnitine, 5-15 g of omega 3 fish oil, 0.1-0.3 g of selenium yeast, and 0.5-2 g of medium-chain fatty acid MCT. VA, VE, carnitine, omega 3 fish oil and medium-chain fatty acid MCT are added into the boar feed disclosed by the invention; furthermore, the micro-ecological preparation in the invention is used in a matched manner; therefore, the boar sperm density and the total sperm number are increased; the sperm deformation rate of breeding boars is obviously reduced; and thus, the breeding performance of boars is greatly improved.

The invention provides a feed additive for improving the reproductive performance of a boar. The feed additive is prepared from the following components in parts by weight of 440-460 parts of pine needles, 40-60 parts of malt, 20-30 parts of silkworm chrysalis, 10-20 parts of herba epimedii, 10-20 parts of actinolite, 40-60 parts of semen cuscutae, 10-20 parts of arginine, 8-12 parts of amino acid chelated zinc and 1-3 parts of vitamin E by virtue of mixing, superfine grinding, uniform mixing and sub-packaging. The particle size of the finished feed additive is less than 10 microns (namely above 300 meshes). The feed additive provided by the invention is high in safety, low in use cost, and relatively good in palatability; the feed additive is added in the ration of the boar according to the quantity of 0.1 percent, the duration of the ejaculation of the boar can be remarkably prolonged, the density and the vitality of the sperms of the boar are improved, and thus the ideal feed additive is used for improving the reproductive performance of the boar.

The invention discloses a diluent for in-vitro low temperature preservation of boar semen and a preparation method thereof. The diluent comprises following components: broad-spectrum antibiotic bacteriostatic agents of D-fructose, 2-amino-2-hydroxymethyl-1,3-propanediol, trisodium citrate, sodium bicarbonate, citric acid, disodium ethylene diamine tetraacetate, N-acetyl-cysteine, skim milk powder, yolk freeze-dried powder, amikacini sulfatis and the like. The diluent for the in-vitro low temperature preservation of the boar semen is suitable for preserving the fresh boar semen at low temperature (4-5 DEG C). The diluent significantly inhibits injury caused to sperm structure during the low temperature preservation process, maintains glucose metabolism and oxidative phosphorylation metabolism processes of boar sperm, effectively prolongs survival time of the boar sperm in the low temperature preservation, and significantly improves use efficiency of the high quality breeding boar semen and economic benefit of breeding.

The invention discloses a breeding boar composite premix for improving reproductive performance; the breeding boar composite premix is prepared from the following raw materials: corn, soybean meal, bran, fish meal, dicalcium phosphate, edible salt, korthalsella japonica, lotus seed, orobanche coerulescens, dioscorea alata, antler glue, gallnut, beautiful millettia root, semen euryales, prepared salt, elaeagnus angustifolia, dogwood, a compound probiotic, and a compound vitamin. The raw materials contain a variety of Chinese herbal medicines, the compatibility is scientific, the roles are complemented with each other, digestion and absorption of pigs on the feed can be promoted, the feed utilization rate and the total nutrient intake are improved, the boar reproductive body function can be improved, breeding boar estrus is regulated and promoted, the hybridization rate of breeding boars is increased, the quality of semen of the breeding boars is increased, and the parturition performance of the childbearing-age boars is increased; with addition of the compound vitamin, the number and vitality of boar sperms can be increased, and generation of sperm deformity is reduced, so that the survival rate and quality of piglets are ensured, and greater economic benefits are brought for raising households.

The invention provides a boar sperm preservative as well as a preparation method and application thereof, and belongs to the technical field of livestock breeding. The boar sperm preservative provided by the invention is prepared from glucose, sodium hydrogen carbonate, sodium citrate, EDTA, citric acid, trihydroxymethyl aminomethane, L-cysteine, levofloxacin, sodium carboxymethylcellulose, bovine serum albumin and Banqing toxin-removing oral liquid. Compared with a conventional diluent Zorlesco, the boar sperm preservative provided by the invention has a better preservation effect of boar sperms, can improve the antibacterial ability of boar semen, and prolongs the storage time of the boar sperms.

The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to boar sperm motility and an application and an acquisition method thereof. Themolecular genetic marker related to boar sperm motility is located at the 8482542 bp position of chromosome 17 of pig, and is a mutation that C is greater than T; the molecular marker of the present application is obtained by single step genome-wide association study (wssGWAS); and the method is simple, efficient and fast.

The invention provides a molecular genetic marker related to the rate of teratosperm of pigs. A molecular genetic marker site is ALGA0108896, the marker site of ALGA0108896 is located at the 24920078bp G>T mutation site in the pig NO.3 chromosome, and a pig reference genome is Sscrofa11.1. According to the molecular genetic marker related to the rate of teratosperm of the pigs, the rates of teratosperm of boars of different genotypes have significant differences, the T allele significantly reduces the rate of teratosperm, the genotype of the ALGA0108896 molecular genetic marker site can be detected to assist the breeding of the boars, homozygous boars with a low rate of teratosperm are selected and reserved, the rate of teratosperm is reduced, and the boar utilization efficiency is effectively improved.

The present invention discloses an environmentally friendly boar feed, including the following ingredients by weight: 40-60 parts of purple rice, 15-25 parts of peanuts, 1-3 parts of lysine, 1-3 parts of an amino acid, 6-10 parts of rapeseed meal, 2-4 parts of peanut oil, 1-3 parts of complex vitamins, 15-25 parts of leeks, 2-4 parts of bone meal, 2-4 parts of nicotinic acid, 5-15 parts of soybean curb residue, 5-7 parts of peanut straw, 4-6 parts of fishmeal, 2-4 parts of ginkgo leaf and 1-3 parts of edible salt, and the environmentally friendly boar feed is reasonable in ratio, can save cost, is nutritious, and can improve boar sperm activity.

The invention discloses boar fodder, being prepared from the following raw materials in parts by weight: 40 to 60 parts of corn flour, 1 to 3 parts of dangshen, 15 to 25 parts of barley, 1 to 3 parts of lysine, 6 to 10 parts of wheat, 1 to 3 parts of aloe, 15 to 25 parts of wheat bran, 2 to 4 parts of soya-bean oil, 5 to 7 parts of tofukasu, 1 to 3 parts of medlar, 2 to 4 parts of mountain flour, 2 to 4 parts of gingko leaves, 2 to 4 parts of bone meal, 6 to 8 parts of weed and 1 to 3 parts table salt. The boar fodder is reasonable in ratio, reduced in cost, rich in nutrition and capable of improving boar sperm motility.

The invention relates to a preparation method of an agonist for improving stock boar sperm activity. The method includes the steps: (1) taking out a boar pig testicular tissue to soak the tissue; (2)cleaning the testicular tissue by PBS buffer solution; (3) adding cell culture fluid to culture the tissue, and centrifuging mixture to remove supernatant; (4) adding hyaluronidase and collagenase IVto vibrate mixture, adding cell culture fluid, and centrifuging mixture to remove supernatant; (5) adding trypsin and deoxyribonuclease to vibrate mixture, and adding cell culture fluid; (6) filter mixture by a cell screen; (7) centrifuging mixture to remove supernatant, and adding cell culture fluid; (8) performing cell culture passage; (9) culturing cell fluid, and storing the cell fluid to obtain the agonist. The agonist has the advantages that the agonist is good in application effect, low in cost and easy to produce.

The invention discloses a boar feed. The boar feed contains corns, bean pulp, fish meal, wheat germ, extruded soybeans, calcium hydrophosphate, mountain flour, salt, 50% choline chloride, a micro-ecological preparation, lysine, methionine, threonine, and 0.5-1 part of a boar premix. Per kilogram of the boar premix contains 3500-12000IU of vitamin A, 750-4500IU of vitamin D, 20-100IU of vitamin E, 4-35 mg of copper, 50-250 mg of iron, 40-150 mg of zinc, 16-80 mg of manganese, 0.2-0.8 g of L-carnitine, 5-15 g of omega 3 fish oil, 0.1-0.3 g of selenium yeast, and 0.5-2 g of medium-chain fatty acid MCT. VA, VE, carnitine, omega 3 fish oil and medium-chain fatty acid MCT are added into the boar feed disclosed by the invention; furthermore, the micro-ecological preparation in the invention is used in a matched manner; therefore, the boar sperm density and the total sperm number are increased; the sperm deformation rate of breeding boars is obviously reduced; and thus, the breeding performance of boars is greatly improved.

The invention discloses a treatment method of boar semen and a use method of the treated boar semen, and belongs to the technical field of artificial insemination of boars, and the treatment method comprises the following steps: after collection, rapidly observing the color of original semen, measuring the pH value and measuring the volume; preserving the temperature of the diluent at 30-35 DEG C for 30-45 minutes, immediately putting the original semen into the diluent, sampling, and evaluating the sperm motility rate and the sperm density; performing isothermal dilution and uniform mixing on the diluent and the original semen, and filling semen bottles with the uniformly mixed diluted semen; and preserving in a dark place at 15-20 DEG C. The treatment method can supplement energy substances for the pig sperms, especially has the special functions of protecting the acrosome structure of the sperms, inhibiting sperm activity before insemination, prolonging the service life of the sperms and improving the insemination capacity, and has the tendentiousness effect of reducing the number of birth of boars and piglets. The method can be used for instant insemination or normal-temperature preservation. After the feed is used, the sperm quality of boars is improved, the conception rate of sows is increased, and the breeding cost is reduced.

The invention relates to the technical field of feed, in particular to a functional composite dietary fiber, a preparation method and application. The functional composite dietary fiber provided by the invention is prepared from composite dietary fiber, composite grease, composite lactic acid bacteria and carriers. Wheat bran in the conventional feed can be replaced; through a cooperated matching effect, the intestinal environment can be improved; the intestinal tract movement can be promoted; the feed utilization rate is improved; the intestine lubrication and bowel relaxing effects can be achieved; meanwhile, the production performance of the functional composite dietary fiber can be enhanced. The functional composite dietary fiber provided by the invention can be added to pig feed of stock boars, replacement sows, pregnant sows and lactation sows; the sperm viability of the boars can be enhanced; the constipation rate of sows is reduced; the milk yield is improved; the piglet survival rate, the piglet body weight and the like are improved.

The invention provides a pig sperm malformation rate related molecular genetic marker. The molecular genetic marker site is H3GA0014627, wherein the H3GA0014627 marker site is the 123567821th bp position of the No.4 chromosome of boar, the 123567821th bp position is a mutation site with C being greater than T, and the pig reference genome is Sscrofa11.1. The molecular genetic marker is used for marking boars of different genotypes. The sperm malformation rate has significant difference, T allele significantly reduces the sperm malformation rate, can assist the breeding of boars by detecting the genotype of the H3GA0014627 molecular genetic marker site, selects homozygous boars with the low sperm malformation rate, and reduces the sperm malformation rate, thereby effectively improves the utilization efficiency of the boars.

A method for preparing an agonist for improving boar sperm motility includes: (1) collecting and infiltrating a testicular tissue of a young boar 3-5 days after born; (2) washing the tissue with PBS; (3) incubating the tissue in a cell culture medium, and centrifuging the cell suspension to remove a supernatant; (4) adding hyaluronidase and collagenase IV followed by shaking, and adding the cell culture medium and centrifuging to remove a supernatant; (5) adding trypsin and deoxyribonuclease followed by shaking, and adding the cell culture medium; (6) filtering the cell suspension by a cell sieve; (7) centrifuging to remove a supernatant, and adding the cell culture medium; (8) culturing and passaging the cells; (9) culturing and storing the cell suspension. The invention has the advantages of good application effect, low cost and simple production process.

The invention relates to a feed for promoting estrus of boars, which belongs to the technical field of production of feeds for pigs and is used for promoting the estrus of boars and improving the boar sperm quality. The feed for promoting the estrus of the boars and improving the boar sperm quality can be used for improving the reproduction performance of boars of child-bearing age. The feed for promoting the estrus of the boars consists of a conventional feed and Chinese herbal medicines and is characterized in that the conventional feed consists of a premix feed consisting of corns, bean pulp, imported fish meal, corn gluten meal, bran, wheat middling, soybean oil, calcium hydrophosphate, mountain flour, salt, lysine, choline chloride and composite vitamins for boars, a composite microelement premix feed and cysteamine; the Chinese herbal medicines include fenugreek grass, actinolite, prepared rehmannia root, cynomorium songaricum and longspur epimedium. According to the feed, on the basis of an optimal formula, the medicine property of the Chinese herbal medicines is sufficiently utilized, the reproduction organism functions of the boars are improved, the estrus of the boars is promoted, the sperm quality of the boars and the reproduction performance of the boars of child-bearing age are improved and the economic benefit is increased.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A / G and G / A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention belongs to the technical field of livestock molecular marker preparation, and concretely relates to a molecular marker related to a sperm activity character of a boar and application. The molecular marker is screened from a PRM2 gene; a nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. An allelic mutation R exists in nucleotide in position 169 of a sequence table, R is G or A, and the mutation causes the polymorphism of NaeI-RFLP. The invention further discloses a primer pair for amplifying PRM2 gene SNP (Single Nucleotide Polymorphism), and the primer pair is also the primer pair for detecting the screened molecular marker provided by the invention. The invention builds a method for detecting the polymorphisms of the sperm activity character of the boar. Association analytic application shows that the molecular marker provided by the invention can be used as the marker for assistant selection of the sperm activity character of the boar.

The invention provides a sperm collecting table for boars. The sperm collecting table is characterized in that sperm collecting fences (2) are arranged on a lifting table (1), a boar driving channel (3) is hinged to an entrance of the sperm collecting fences and is vertically rotationally hinged to the sperm collecting fences, and pulleys (4) are arranged at contact points of the boar driving channel and the ground. The sperm collecting table has the advantages that the problem that sperm collecting workers often need to squat and stoop down for collecting sperm of boars in boar sperm collecting procedures, are easy to get tired due to long-term squatting and stooping actions and are easy to suffer from occupational diseases can be solved; the lifting table is arranged at the bottoms of the sperm collecting fences, the heights of the sperm collecting fences can be adjusted by the aid of the lifting table, accordingly, workers can collect sperm of the boars while standing, the labor intensity can be effectively relieved for the sperm collecting workers, and shortcomings due to the traditional sperm collecting modes can be overcome.

The invention belongs to the technical field of pig molecular marker screening, and in particular relates to a molecular marker associated with boar sperm concentration traits. The molecular marker is cloned from a gene fragment with accession number WU_10.2_17_4574599, and the gene is typed by gene chip technology , screened to obtain a molecular marker associated with pig sperm concentration traits, its nucleotide sequence is shown in SEQ ID NO: 1, there is a T / C allelic mutation at the 101st base of the sequence , when the 101st nucleotide of SEQ ID NO: 1 is C, it is determined that the pig has a higher sperm concentration. The invention provides a new SNP molecular marker resource for marker-assisted selection of pig semen traits.

The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to linear motion of boar sperm, and an application and a method thereof. The method analyzes the linear motion traits of boar sperm by genome-wide association study (wssGWAS) analysis, and obtains three molecular genetic markers related to linear motion of boar sperm, which are located at the 136112947 bp position of chromosome 15 of pig, and is a mutation that T is greater than C; at the 18505448 bp position of chromosome 3 of pig, and is a mutation that A is greater than G;and at the 63272581 bp position of chromosome 11 of pig, and is a mutation that C is greater than T. The method used to analyze the linear motion of boar sperm is simple, efficient and fast.

The invention discloses a mixed feed for improving antioxidant activity of the sperm of a boar. The mixed feed is characterized by comprising the following raw materials by weight: 40 to 60% of corn, 5 to 20% of fish meal, 5 to 20% of wheat, 10 to 25% of wheat bran, 0.2 to 5% of a soybean small peptide additive, 0.05 to 2% of taurine, 2 to 10% of soybean meal, 2 to 8% of plant oil, 1 to 2% of a calcium element additive, 0.1 to 0.8% of salt, 0.05 to 0.5% of N-carbamylglutamic acid, 0.1 to 0.3% of a composite trace element additive, 0.03 to 0.08% of a composite vitamin additive, 0.005 to 1% of anthocyanidin, 0.05 to 2% of tea polyphenol, 0.5 to 1.5% of amino acid and 0 to 5% of bentonite. The mixed feed is applicable to the boar, can effectively increase the amount of boar seminal fluid, improve sperm vitality and sperm density, reduce the rate of sperm deformation and enhance antioxidant activity of the sperm of the boar and is of great significance to improvement of the quality of boar seminal fluid.