14 results about "Boar sperm" patented technology

The application discloses six boar sperm motility miRNA markers, which are ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p, ssc-miR-199a-3p and ssc-miR-31, wherein the ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p and ssc-miR-199a-3p are highly expressed in low-motility semen sperm plasma exosomes and lowly expressed in high-motility semen sperm plasma exosomes, and the ssc-miR-31 is lowly expressed in low-motility semen sperm plasma exosomes and highly expressed in high-motility semen sperm plasma exosomes. The application has good prospects for identifying boar sperm motility, and the disclosed miRNA markers have accuracy and reliability.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The invention discloses a special biscuit for improving sperm fertilization ability of boars under heat stress and a processing method thereof, and the biscuit comprises the following components by weight: 10% of grease; the grease comprises DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid), and the weight ratios of the DHA and the EPA in the grease are respectively 30% and 35%; 8%-18% of an amino acid component; the weight ratio of lysine to methionine to tryptophan to threonine to arginine in the amino acid component is 100 to (26 to 38) to (18 to 32) to (70 to 90) to (60 to 120); the vitamin component comprises 0.6%-1.0% of vitamin A, 1%-3% of L-carnitine, 0.5%-1% of vitamin E and 0.5%-1% of vitamin D; the trace element components comprise 0.01%-0.1% of nano selenium, 0.1%-1% of nano zinc and 0.02% of organic chromium; and the balance of corn starch. The special biscuit can improve the sperm motility of the boars and improve the reproductive nutrition status of the boars, the special biscuit is designed into independent quantitative solid blocks, and the problems that powdery additives are inaccurate in metering, uneven in mixing and waste in throwing can be solved.

The application discloses a pig IZUMO2 eukaryotic protein and a preparation method and application of a monoclonal antibody of the pig IZUMO2 eukaryotic protein. The full-length sequence of pig IZUMO2 nucleotide CDS is verified, a research method for removing the transmembrane structure of the pig IZUMO2 nucleotide CDS and only expressing the extramembrane sequence of the pig IZUMO2 nucleotide CDS in eukaryotes is designed, and the IZUMO2 protein is expressed and secreted by CHO-K1 cells. The protein expressed by the method is close to natural protein, has biological activity and is simple to operate. The qPCR and ELISA tests verify that the IZUMO2 protein can reduce the expression of inflammatory factors in RAW264.7 cells induced by LPS, and has a certain anti-inflammatory capacity. It is found through experiments that the IZUMO2 protein can promote the capacitation of boar sperm, and it is proved that the protein can be applied to the preparation of a reagent for promoting the capacitation of boar sperm.

The invention belongs to the technical field of biology. The invention relates to an antioxidant composition, in particular to an antioxidant composition and application thereof in normal-temperature preservation of boar semen. Researches find that the activity of boar sperms in the normal-temperature preservation period can be effectively improved by independently adding lycopene or antioxidant SKQ1, the combined use effect of lycopene and antioxidant SKQ1 shows a synergistic effect, and when the boar sperms are preserved to the seventh day, the average sperm activity value exceeds 70%. In addition, through combined treatment, the integrity of sperm acrosome and plasma membrane is remarkably maintained, the antioxidant enzyme activity of sperms is improved, the active oxygen and malonaldehyde level are effectively reduced, meanwhile, the mitochondrial membrane potential and the adenosine triphosphate content are remarkably improved, and the expression level of sperm P-AMPK and Nrf2 protein is improved. In conclusion, the lycopene and the SKQ1 are combined for use, oxidative stress and energy metabolism are synergistically regulated and controlled, the quality and function stability of the boar sperms in the normal-temperature preservation process are remarkably improved, and the lycopene and the SKQ1 have good application value and application prospects.

The invention relates to the technical field of boar breeding, and discloses application of a molecular marker in boar sperm malformation heat stress resistance screening, the molecular marker is located in a CIB4 gene of a No.3 chromosome 112.2-113.2 Mb region, the mutation site is 112656241bp, and T is greater than C. The relationship between the molecular marker and the sperm malformation heat stress resistance of the boar is determined by application, and a guiding effect is provided for genetic breeding related to the sperm malformation heat stress resistance of the boar. Meanwhile, the invention also discloses a method for screening the molecular marker.

The invention relates to an additive of a diluent for preserving boar semen at 17 DEG C and an application method thereof. Myoinositol with the concentration of 1 mg / mL is added into a boar semen diluent, so that the activity and forward movement proportion of boar sperms under the preservation condition of 17 DEG C can be remarkably improved, and meanwhile, the lipid oxidation state and acrosome integrity of the boar sperms are not influenced. The method is easy and convenient to operate, low in cost, suitable for the large-scale pig farm artificial insemination technology and beneficial to prolonging the pig semen preservation time and improving the breeding efficiency.

The invention relates to the technical field of animal genetic breeding, and particularly discloses a molecular marker group for identifying freezing tolerance of pig sperms and application of the molecular marker group, the molecular marker group comprises three specific SNP marker groups: a group A is a Duroc pig specific marker and comprises 10 SNPs such as rs322459109; the group B is a large white pig specific marker and comprises 33 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like; and the group C is a cross-variety universal marker and comprises 12 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like. The markers are located in the gene regions of PARN, SOX9, CCDC181, DHH, NCKAP5L, MAP2K6, MPV 17 and DRC1. The invention further provides a kit containing the detection primer and / or probe, a boar sperm freezing tolerance evaluation method, a boar breeding method, application of candidate genes, a computer readable storage medium and electronic equipment, early-stage, rapid and accurate evaluation of boar sperm freezing tolerance can be achieved, the breeding efficiency is remarkably improved, the breeding cost is reduced, and the method is suitable for large-scale popularization and application. And an important technical support is provided for industrial application of a pig frozen semen technology.

The invention provides an SNP molecular marker associated with a porcine sperm resuscitation rate and application of a detection substance of the SNP molecular marker in porcine genetic breeding, the SNP molecular marker is named as rs343360383 and is located at a nucleotide site of 3367909bp of a porcine chromosome 5 of a reference sequence of an international porcine genome version 11.1, the basic group of the site is A or T, the molecular marker rs343360383 is located at the upstream 3671bp position of a PPARA gene, and the molecular marker rs343360383 is located at the upstream 3671bp position of the PPARA gene. And the gene corresponds to the 101bp position in the nucleotide sequence as shown in SEQ ID NO: 1. The SNP molecular marker can assist in improving the sperm recovery rate of the breeding boars, and a new marker resource is provided for auxiliary selection of the breeding boars.

The invention relates to the technical field of biological breeding, and discloses an application of exogenous miR-92a or a simulant or an analogue in improving the preservation quality of fresh boar sperms.The exogenous miR-92a or the simulant or the analogue is added into the fresh boar sperm preserved at normal temperature through the technology, PI3K / AKT signal channels are activated by down-regulating expression of PTEN, and the preservation quality of the fresh boar sperm is improved. The effects of improving the sperm motility, improving the sperm plasma membrane intact rate and improving the semen quality can be achieved.

The invention relates to a live pig sperm morphology intelligent identification method and system based on an improved YOLOv8 model, and the method comprises the following steps: collecting a boar sperm image sample, and marking the sperm image sample; preprocessing the labeled image data, uniformly formatting the image and a labeled file, converting the image and the labeled file into a labeled file in a YOLO format, and generating a training set and a verification set; an improved YOLOv8 target detection model is adopted, and on the basis of a YOLOv8m version, the annotation data are trained; in a sperm morphology detection task, a recall rate and mAP (at) 0.5 are used as evaluation indexes, a sperm target can be rapidly detected in an image through a YOLOv8 model obtained through training, and different sperm morphologies can be accurately identified; according to the intelligent identification method and system for the sperm morphology of the live pig based on the improved YOLOv8 model, large-scale sperm samples can be automatically processed through an efficient image identification and analysis technology, errors caused by manual operation are reduced, and the accuracy and consistency of detection are improved.

The application discloses a SNP genetic marker related to a pig semen quality trait and an application thereof, the SNP genetic marker is located in a promoter region of a pig ANXA5 gene, and a nucleotide sequence of the genetic marker is shown in Table SEQ ID NO.1; there are three SNP sites on the sequence respectively, and the three SNP sites are as follows: a base Y at 169bp of the sequence is C or T, a base M at 171bp is C or A, and a base K at 740bp is G or T.Through correlation analysis, the three SNP sites located in the promoter region of the ANXA5 gene are all related to boar sperm motility, and are in a linkage disequilibrium state, further haplotype analysis divides the three SNP sites into three haplotypes, and significant differences are found in the boar sperm motility trait after correlation analysis.The haplotypes can screen boars with high semen quality traits, and improve economic benefits of pig breeding.

The invention discloses a functional artificial egg capable of reducing the malformation rate of sperms of boars and a preparation method thereof, and every kilogram of egg liquid in the artificial egg comprises the following components in percentage by weight: 25-35% of protein component; 20-28% of a fatty acid component; 25-30% of phospholipid and 4-5% of cholesterol; the vitamin components comprise 500 to 1000 [mu] g / kg of vitamin A, 5 to 10 [mu] g / kg of vitamin D, 80 to 120 [mu] g / kg of vitamin E, 3 to 5 [mu] g / kg of vitamin B12 and 1500 to 2500 mg / kg of carnitine; the mineral element components comprise 650 to 750 mg / kg of phosphorus, 450 to 550 mg / kg of potassium, 800 to 1000 mg / kg of calcium, 40 to 60 mg / kg of magnesium, 18 to 20 mg / kg of iron, 6 to 12 mg / kg of zinc and 120 to 180 [mu] g / kg of selenium; and the balance of water. On the basis of nutritional physiology of spermatogenesis of boars, protein components, fatty acid components, vitamins (A, D, E, B12 and carnitine) related to oxidation resistance / energy metabolism and mineral elements (zinc and selenium) are accurately compounded; the components have a synergistic effect in the aspects of promoting the integrity of a sperm cell membrane structure, providing an energy metabolism substrate and enhancing an anti-oxidation defense system, so that the sperm malformation rate is remarkably reduced.

The Mogroside extract has the effect of improving the damage of the heat stress boar's reproductive capacity. Research shows that, in the process of heat stress of boar, by adding Mogroside extract in the basic diet, the damage of reproductive capacity caused by heat stress can be effectively improved. The test data shows that the Mogroside extract can significantly improve the sperm density, effective sperm number, sperm motility and reduce the sperm abnormality rate of boar; significantly increase the content of a-glucosidase, lactate dehydrogenase, acrosome enzyme and hyaluronidase in the semen, and increase the concentration of testosterone in the serum, enhance the energy supply, structural integrity, functional maturity and fertilization capacity of sperm. Therefore, the Mogroside extract has the potential for further development in the treatment and prevention of heat stress boar's reproductive capacity damage, and can be used for preparing the medicine for improving the heat stress boar's reproductive capacity, so as to further develop the health care function and medicinal value of Siraitia grosvenorii.