Sample-adding pen and sample-adding method and application thereof
A sample adding pen and micro sample adding technology, applied in the application field of biochemical technology, can solve the problems of large volume of the pipette, high cost of the pipette, unfavorable promotion, etc. Effect
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[0033] The structure of the biological sampling pen is as follows: figure 1 As shown, it consists of 6 parts. The tip 1 mainly provides the support point for the ball 3. The pen holder 2 is used to store the filler 5. When the ball 3 rubs and rotates on the base, it provides enough shearing force to drive the inner part of the biological sampling pen. The filling 5 flows out, the connecting device 4 is used to connect the pen holder 2 and the pen head 1, the filling 5 is the sample to be added, and the sealing oil 6 prevents the sample from volatilizing. When the biological sample pen writes on the substrate (similar to the writing process of a ballpoint pen or gel pen), the ball 3 at the front end of the pen head 1 rotates to drive the filling 5 to flow out.
[0034] The details are as follows:
[0035] Step 1: Inject the sample into the barrel of the biosampling pen to form a filling, and then seal it with mineral oil (sealing oil). There are a wide variety of samples here...
example 1
[0037] Example 1 Quantitative writing of aqueous solution on A4 paper with biological sample pen
[0038] Step 1: Inject the aqueous solution into the barrels of three different types of biological sampling pens to form fillers. The models (referring to the diameter of the ball) are 0.38mm, 0.5mm, and 0.7mm respectively, and seal with mineral oil after filling.
[0039] Step 2: Hold three biological sampling pens and write on A4 paper at a speed of 2cm / s and 4cm / s. The steel ball (ball) at the tip of the pen rubs and rotates on the A4 paper, and the aqueous solution is subjected to shear force and flows out to A4 paper. The results show that the larger the ball at the tip of the pen, the more aqueous solution per unit length of the sample added (see image 3 ). image 3 The standard deviation in is very small, indicating that the biological sampling pen has high sampling accuracy.
[0040] Step 3: Adjust the amount of aqueous solution sample added by changing the writing sp...
example 2
[0043] Example 2 Quantitative writing of rhodamine B solution on A4 paper with biological sample pen
[0044] Step 1: Inject Rhodamine B solution into three different types of biological sampling pens to form fillers, the models are 0.38mm, 0.5mm, and 0.7mm, and then seal with mineral oil.
[0045] Step 2: Hold three biological sampling pens and write on A4 paper at a speed of 2cm / s and 4cm / s. The steel ball (ball) at the tip of the pen rubs and rotates on the A4 paper, and the rhodamine B solution is subjected to shear force , so that it flows out onto A4 paper. The results show that the larger the ball at the front end of the bio-sampling pen, the wider the width of the sample (see Image 6). This proves that the sample adding pen can not only control the sample volume, but also control the plane spatial distribution (width). It can be used for fast sample addition of heavy metal elements such as gold, gallium, mercury, antimony (V), thallium (III) and so on.
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