Stable glycated serum protein detection reagent and application thereof

Abstract

The invention provides a stable glycated serum protein detection reagent and application thereof. The reagent adopts double reagents. By storing nitroblue tetrazolium (NBT) in the neutral pH environment of a reagent R1, the problems that the NBT is easily separated out in an alkali environment, the system is turbid and the like are solved. Moreover, by adopting alkyl glycoside APG1214 serving as a new nonionic surfactant, the measurement performance is significantly improved, and a good effect on keeping the system transparent is achieved. The reagent is good in accuracy and stability, and can completely meet the clinical requirements.

Description

technical field

[0001] The invention relates to a detection reagent for clinical determination of the content of glycosylated serum protein in serum and plasma and its application. Background technique

[0002] Glycated serum protein (also known as fructosamine) is a non-enzymatic glycation reaction product of various proteins in serum under the action of high sugar. Serum protein glycation mainly occurs on lysine residues of protein molecules, among which albumin is the Mainly, the half-life of serum albumin is 17-19 days, and the measurement of GSP can reflect the average blood sugar level of 2 to 3 weeks before the measurement, so it is of great significance in the diagnosis of diabetes mellitus (DM) in the short-term monitoring and efficacy evaluation .

[0003] At present, there are many methods for detecting GSP, but each has its own advantages and disadvantages. For example, the chromatography method has cumbersome operation steps and requires high equipment requirem...

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