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Nutrient and nontoxic culture medium of pot plant and preparation method thereof

A cultivation substrate and nutrition technology, applied in the comprehensive field of horticultural technology and experimental zoology, can solve the problems of undetectable microorganisms, etc., and achieve the effects of low manufacturing cost, environmental protection, large-scale production and market sales

Inactive Publication Date: 2013-08-07
EAST CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germ-free mice In order to utilize the existing detection technology, no microorganisms can be detected inside and outside the germ-free mice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0012] The preparation method of the potted plant nutrient non-toxic cultivation substrate of the present invention, the steps comprise:

[0013] (1) Preparation of SPF grade or sterile grade mouse litter: Add corn cob litter to the polypropylene plastic box, after autoclaving, use it to raise 5-7 mice, replace the cage box after 7-10 days, and collect Litter containing mouse excrement was obtained.

[0014] Specifically, 150 grams of corncob padding was added to a 294*195*125mm polypropylene PP plastic box, and after autoclaving, it was used to raise 5-7 mice. After 7-10 days, the cage box was replaced, and the mice containing Bedding for excrement.

[0015] Specifically, when the mice are SPF mice, cobalt 60 is used to irradiate and sterilize the feed. When the mice are sterile-grade mice, use autoclaved feed, and the autoclaved conditions are 0.1-0.15Mpa, 121°C, 20-30min. The drinking water of the mice was purified water sterilized by autoclaving, and the autoclaving con...

Embodiment 1

[0026] (1) SPF grade or sterile grade mouse litter collection: 294*195*125mm polypropylene PP plastic box, add 150 grams of corncob litter, after high-pressure steam sterilization, (sterilization conditions are 0.15Mpa, 121°C, 20-30min) is used to feed 5-7 mice, and the cage box is replaced after 7-10 days, and the bedding material containing mouse excrement is collected. SPF grade mice use cobalt 60 irradiation sterilized feed, sterile grade mice use high pressure sterilized feed (sterilization conditions are 0.1Mpa, 121 ℃, 20min), drinking water is purified water after high pressure steam sterilization (0.15Mpa , 121°C, 20min).

[0027] (2) Cake material processing: buy peanut cake or sesame cake, crush it and pass it through a 1-2mm sieve, autoclave it for later use. Sterilization conditions are the same as feed.

[0028] (3) Mixing: mix the litter collected in step (1) and the treated cake in step (2) according to the weight ratio of mouse waste litter: cake = 3: 1.5, tu...

Embodiment 2

[0035] (1) SPF grade or aseptic grade mouse litter collection: 294*195*125mm polypropylene PP plastic box, add 150 grams of corn cob litter, after autoclaving, (sterilization conditions are 0.15Mpa, 121°C, 20 -30min) is used to feed 5-7 mice, replace the cage box after 7-10 days, and collect the bedding material containing mouse excrement. SPF grade mice use cobalt 60 irradiated sterilized feed, sterile grade mice use high-pressure sterilized feed (sterilization conditions: 0.1Mpa, 121°C, 20min), drinking water is purified water after high-pressure sterilization (0.15Mpa, 121°C, 20 min).

[0036] (2) Cake material processing: buy peanut cake or sesame cake, crush it and pass it through a 1.5mm sieve, autoclave it for later use. Sterilization conditions were the same as mouse feed.

[0037] (3) Mixing: mix the litter collected in step (1) and the treated cake in step (2) according to the weight ratio of mouse waste litter: cake = 4:2, turn it over 4 times to make it evenly mi...

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Abstract

The invention discloses a nutrient and nontoxic culture medium of a pot plant and a preparation method thereof. The preparation method includes: autoclaving and then mixing an SPF-grade (specific pathogen free-grade) or sterile-grade mice bedding material and a cake material; and subjecting the mixed material to sealed fermentation, sun-baking, crushing, filtering and uniform mixing with ferrous sulfate to obtain the nutrient and nontoxic culture medium of the pot plant. The weight ratio of the SPF-grade or sterile-grade mice bedding material and the cake material is (3-5):(1.5-2.5). According to the nutrient and nontoxic culture medium of the pot plant and the preparation method thereof, the SPF-grade or sterile-grade waste animal bedding material is utilized for providing the nutrient and nontoxic culture medium for the pot plant, so that environmental friendliness, low cost and easy operation are achieved and mass production and marketing can be facilitated.

Description

technical field [0001] The invention belongs to the comprehensive field of horticultural technology and experimental zoology, in particular to a potted plant nutrient non-toxic cultivation substrate and a preparation method thereof. Background technique [0002] The microbiological grades of the excrement of SPF and germ-free mice belong to clean excrement. SPF mice are specific pathogen-free mice, which not only exclude the main zoonotic pathogens and severe zoonotic disease pathogens, but also exclude the main weak pathogenic pathogens and recessive and latent infection pathogens of animals. Germ-free mice In order to use the existing detection technology, no microorganisms can be detected inside and outside the germ-free mice. The microbiological grades of these two grades of mouse excrement are consistent with those of mice, and both belong to clean excrement. In the prior art, discarded mouse bedding is either thrown away as ordinary garbage, or sent to a harmless cen...

Claims

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Application Information

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IPC IPC(8): A01K1/015A01K67/033A01G31/00
Inventor 马雪云
Owner EAST CHINA NORMAL UNIVERSITY
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