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Method of singulating embryos

An embryo, human embryo technology, applied in the fields of botany equipment and methods, analytical materials, plant regeneration, etc., can solve problems such as expensive, time-consuming and labor-intensive

Inactive Publication Date: 2013-08-07
WEYERHAEUSER NR CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a non-limiting example, such manual operations are time-consuming, labor-intensive, and therefore expensive

Method used

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  • Method of singulating embryos
  • Method of singulating embryos
  • Method of singulating embryos

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Embodiment Construction

[0015] figure 1 Schematically depicted is an automated system 20 for implementing a method for single isolated embryos, according to one embodiment of the present invention. The system 20 is suitably mounted in conjunction with an assembly (not shown) for assembling the artificial seed, or at a remote distance from such an assembly.

[0016] System 20 includes embryo storage assembly 22 , programmable logic controller (PLC) 24 , placement mechanism 26 , and embryo deposition assembly 28 . Embryo storage assembly 22 includes single separation container 30 , elevator mechanism 32 , and sensor 34 . The single separation vessel 30 is suitably a vessel having a plurality of embryos 40 suspended in a fluid such as sterile ultrapure water. Preferably, the fluid is agitated sufficiently to suspend all embryos 40 . The single separation container 30 is mounted on an elevator mechanism 32 .

[0017] The elevator mechanism 32 includes a base plate 50 connected to a known elevator 52 ...

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Abstract

A method of singulating embryos is provided. The method includes providing a plurality of embryos (40) within a system (20) and sensing (34) at least one of the plurality of embryos in a fluid. The method also includes dispensing (26) at least one of the plurality of embryos on a surface (28).

Description

[0001] This application is a divisional application of the patent application with application number 201010299388.1 submitted on September 27, 2010. technical field [0002] The present invention provides a method of singulating embryos. Background technique [0003] Vegetative reproduction of plants has been shown for some species to produce large numbers of genetically identical embryos, each capable of growing into normal plants. Such embryos usually must be further cultivated under laboratory conditions until they reach the autotrophic "shoot" state, which is characterized by producing their own food through photosynthesis, resisting drought, producing nutrients that can penetrate soil root, and the ability to resist soil microorganisms. Some researchers have experimented with the production of artificial seeds, called artificial seeds, in which individual plant somatic cells or zygotic embryos are encapsulated in the seed coat. Examples of such artificial seeds are d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCA01H4/006A01H4/00G01N33/48735
Inventor 安东尼·P·斯万达
Owner WEYERHAEUSER NR CO