Induction method of nitraria loose embryonic callus

An embryogenic callus and callus technology is applied in the field of induction of loose embryogenic callus of A. The effect of premature aging and simplified procedures

Inactive Publication Date: 2013-08-14
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

[0003] Because Nitraria is limited by the genetic resources of this species, some new excellent traits cannot be produced, and the efficiency of general mutation breeding is extremely low, so it is difficult to induce new excellent traits, although gene transformation technology can introduce target genes into Nitraria genome, but because transgenic plants are difficult to release in the environment, this technology only stays in the laboratory stage

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  • Induction method of nitraria loose embryonic callus
  • Induction method of nitraria loose embryonic callus
  • Induction method of nitraria loose embryonic callus

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[0023] The following examples are used to further illustrate the present invention. The following examples are illustrative and not restrictive. The following examples cannot be used to limit the scope of protection of the present invention.

[0024] Method for inducing loose embryogenic callus of Nitraria

[0025] ⑴Take one-year-old shoots of Nitraria tangutorum seedlings, sterilize them with 40% Antifomin, and inoculate them on 1 / 2MS medium for cultivation and subculture of sterile seedlings;

[0026] ⑵Cut the stem segment (without leaves) between the two leaves of the sterile Nitraria seedlings, and inoculate them to 1 / 2MS+0.5mg / L (2,4-D)+100mg / L (Vc)+20g / L sucrose The loose callus was induced on the culture medium, the culture condition was 25°C, the light was 12 hours per day, and the light intensity was 1000 lux.

[0027] Among them, Vc is added at 50°C after the medium is melted, and Vc is sterilized by suction filtration in advance.

[0028] (3) After three weeks, inoculate the...

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Abstract

The invention relates to an induction method of nitraria loose embryonic callus. The method comprises the following steps of: (1) scissoring a stem between two blades of a nitraria aseptic seedling, and inoculating the stem to a culture medium for carrying out induction of loose callus; (2) after 2-3 weeks, inoculating the induced loose nitraria callus to the culture medium for carrying out induction of embryonic callus; in the process of inducing the loose callus to the embryonic callus, carrying out successive transfer culture once per 5-8 days; and in the process of successive transfer culture, transferring callus blocks; and (3) carrying out successive transfer culture for 4-5 times so that the loose nitraria callus is changed to relatively hard callus, the color gradually changes from transparent white to milk white, yellow and finally peak green, and at the moment, viewing from the organizational structure, the callus is of a loose structure formed by many spheroids. When the callus with the characteristics occurs, the induction of the nitraria embryonic callus is finished. According to the method, by inducing the nitraria embryonic callus, an induction system of the standard nitraria loose embryonic callus is established.

Description

Technical field [0001] The invention belongs to the fields of agriculture and plant breeding, and particularly relates to a method for inducing loose embryogenic callus of Nitraria tangutorum. Background technique [0002] Nitraria L., shrubs, Zygophyllaceae, there are 12 species in the world, 8 species in my country, mainly distributed in arid desert areas, and its fruits are rich in vitamins and amino acids, and have high medicinal properties And edible value. According to the survey, Nitraria tangutorum has male sterility, and the interspecific hybridization is chaotic, and the differentiation is serious. There are big differences in the size, color and taste of the fruit. Therefore, the research on the breeding technology of Nitraria in vitro has very important practical significance for the breeding and resource development of wild Nitraria resources. [0003] Because Nitraria tangutorum is restricted by the genetic resources of this species, some new excellent traits cannot ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨静慧刘艳军黄俊轩李建科武春霞
Owner TIANJIN AGRICULTURE COLLEGE
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