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Preparation method of freeze-dried egg yolk antibody

An egg yolk antibody and freeze-drying technology, which is applied in the field of preparation of freeze-dried egg yolk antibody, can solve the problems of sharp drop in ultrafiltration membrane water permeability, low product specification content, long freeze-drying time, etc., and achieve a reduction in freeze-drying time and energy consumption , High antibody concentration ratio and short freeze-drying time

Active Publication Date: 2015-06-10
重庆永健生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of concentrating purified egg yolk antibody (molecular weight 180kD) by ultrafiltration includes filtering and concentrating the purified egg yolk antibody through an ultrafiltration membrane stack or a hollow fiber ultrafiltration column with a molecular weight cut-off of 100kD. increase, the solution on the liquid inlet side becomes more viscous, and the water permeability of the ultrafiltration membrane decreases sharply. Generally, the original solution can only be concentrated by 5 to 15 times, resulting in a relatively large volume of the final freeze-dried product. The freeze-drying machine per unit area The number of effective freeze-drying bottles is small, and the product specification content is low (for example, taking the duck virus hepatitis freeze-dried egg yolk antibody approved by the Ministry of Agriculture as an example, 15mL antibody concentrate is divided into 25mL freeze-drying control bottles for 250 chicks duck treatment), long freeze-drying time and high energy consumption

Method used

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  • Preparation method of freeze-dried egg yolk antibody
  • Preparation method of freeze-dried egg yolk antibody
  • Preparation method of freeze-dried egg yolk antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1.1 Method:

[0040] 1. Concentration:

[0041] (1) Take 1500L of refined goose plague egg yolk antibody, weigh polyaluminium chloride according to the mass volume ratio of polyaluminum chloride and refined goose plague egg yolk antibody 1:1, that is, 1.5kg, dissolve it with 3000mL normal saline and add it , you can stir while adding. At this time, the refined gosling plague egg yolk antibody solution turns from clear and transparent to turbid. After standing for 15 minutes, use a plate and frame filter to add a filter plate for coarse filtration, and the filtrate is filtered with a plate and frame filter. plate and a filter membrane with a filtration precision of 0.45 μm for fine filtration, and the fine filtrate is filtered and sterilized with a microporous filter element filter with a filtration precision of 0.22 μm, and then the primary filtrate is obtained;

[0042] (2) Concentrate the primary filtrate obtained in step (1) by using a hollow fiber ultrafilter to ob...

Embodiment 2

[0053] 2.1 Method:

[0054] 1. Concentration:

[0055] (1) Take 1500L of refined goose plague egg yolk antibody, weigh polyaluminum chloride according to the mass volume ratio of polyaluminum chloride and refined goose plague egg yolk antibody 1:1.3, that is, 1.15kg, dissolve it in 2500mL of normal saline and add it , you can stir while adding. At this time, the refined gosling plague egg yolk antibody solution turns from clear and transparent to turbid. After standing for 15 minutes, use a plate and frame filter to add a filter plate for coarse filtration, and the filtrate is filtered with a plate and frame filter. plate and a 0.45 μm filter membrane, and the fine filtrate is filtered and sterilized by a microporous filter element filter with a filtration accuracy of 0.22 μm, and then the primary filtrate is obtained;

[0056] (2) Concentrate the primary filtrate obtained in step (1) by using a hollow fiber ultrafilter to obtain the final concentrate:

[0057] The hollow fi...

Embodiment 3

[0068] 3.1 Method:

[0069] 1. Concentration:

[0070] (1) Take 1500L of refined goose plague egg yolk antibody, weigh polyacrylamide according to the mass volume ratio of polyacrylamide and refined goose plague egg yolk antibody 1:0.8, that is, 1.88kg, dissolve it with 3000mL normal saline and add it, you can Stir while adding. At this time, the refined gosling plague egg yolk antibody solution changes from clear and transparent to turbid. After standing for 15 minutes, use a plate and frame filter to add a filter plate for coarse filtration, and the filtrate is to use a plate and frame filter to add a filter plate and 0.45μm filter membrane fine filtration, the fine filtrate is filtered and sterilized by a microporous filter element filter with a filtration accuracy of 0.22μm, and then the primary filtrate is obtained;

[0071] (2) Concentrate the primary filtrate obtained in step (1) by using a hollow fiber ultrafilter to obtain the final concentrate:

[0072] Sterilize t...

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Abstract

The invention provides a preparation method of a freeze-dried egg yolk antibody. A refined egg yolk antibody solution used as a raw material is concentrated and freeze-dried to obtain the freeze-dried egg yolk antibody. The preparation method is characterized in that the technical process of the concentration comprises the following steps: (1) taking the refined egg yolk antibody solution, adding flocculant, standing, filtering, and sterilizing to obtain a primary filtrate; and (2) performing ultrafiltration and concentration on the primary filtrate obtained in the step (1) through a hollow fiber ultrafilter, thus obtaining a final concentrated solution. Compared with the prior art, the preparation method provided by the invention is high in concentration multiple, low in preparation cost, short in freeze-drying time, simple in process and suitable for large-scale antibody concentration.

Description

technical field [0001] The invention relates to a preparation method of freeze-dried egg yolk antibody. Background technique [0002] Antibody refers to a type of globulin that is synthesized and secreted by plasma cells after B cells in the body are activated, proliferated, and differentiated into plasma cells after being stimulated by foreign antigens, and can specifically recognize and bind to corresponding antigens. Immunizing laying hens with specific antigens can also produce corresponding specific antibodies, which are transferred from blood to egg yolk. This specific antibody is called egg yolk antibody, which has therapeutic and emergency preventive effects on certain poultry diseases. [0003] Egg yolk contains 48% water, 17.8% protein, and 30.5% fat. Almost all of the fat is combined with protein to form lipoproteins. Among them, the three active proteins α, β, and γ are water-soluble proteins, and egg yolk antibodies are water-soluble γ protein. [0004] The pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/02C07K16/08C07K1/36C07K1/34C07K1/30
Inventor 张立武关受江
Owner 重庆永健生物技术有限责任公司
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