Echinocandin B high-producing strain and application thereof

A technology of echinocandin and high-yield strains, applied in the field of bioengineering, can solve the problems of poor selectivity, affecting the yield of anidungin, and low sensitivity, and achieve stable high yield, good industrial application value, and stable genetic traits Effect

Active Publication Date: 2013-09-11
NOVOCODEX BIOPHARMACEUTICALS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] First, because the targets of these two types of drugs are fungal cell membranes, their selectivity is poor, resulting in relatively large toxic and side effects;
[0005] The second is the reduced efficacy of these drugs due to the widespread emergence of clinically resistant fungi;
[000

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Stability investigation of mutant strains

[0032] Medium:

[0033] Slant / Plate Medium: Potato Dextrose Agar Medium.

[0034] Seed medium (wt): glucose 1.0%, glycerol 1.0%, cottonseed meal 2.5%.

[0035] Fermentation medium (wt): maltose 6.0%, dextrin 2.0%, cottonseed flour 3.0%, yeast powder 1.5%, oil 0.2%, glutamic acid 0.3%, KH 2 PO 4 0.1%.

[0036] Training conditions:

[0037] Incline cultivation temperature is 28°C, cultivation time is 5 days;

[0038] Seed liquid culture temperature 25 ℃, culture 3 days;

[0039] The culture temperature of the fermentation broth was 25° C. and cultured for 7 days. The rotation speed of the shaker flask was 220rpm / min.

[0040] Five flasks were cultured in shake flasks for each generation of strains, and parallel experiments were carried out. The fermentation product was detected by HPLC, and the average value of the measured content was taken.

[0041] Fermentation broth treatment process:

[0042] Add 8ml...

Embodiment 2

[0060] The comparison of embodiment 2 mutant strains and starting bacterial strains

[0061] Medium, culture condition and detection method, as embodiment 2.

[0062] The comparison between the mutant strain and the starting strain, taking the echinocandin B content as an example, the comparison results are shown in Table 3:

[0063] Table 3 Comparison of mutant strains and starting strains

[0064] bacteria Starting strain mutant strain Echinocandin B content (mg / l) 60 823 Relative amount 100% 1372% relative increase —— 12.7 times

[0065] It can be seen from Table 3 that the echinocandin B content of the mutant strain prepared by the present invention is relatively increased by 12.7 times compared with the echinocandin B content of the starting strain.

[0066] By fermenting the high-yield bacterial strain provided by the invention, echinocandin B can be obtained in high yield, thereby ensuring the output of anidulungin and...

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PUM

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Abstract

The invention discloses an Echinocandin B high-producing strain which is a mutant strain of Aspergillus rugulovalvus ATCC 58398. The strain is collected in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms and has the collection number of CGMCC (China General Microbiological Culture Collection Center) No. 5413. The invention also discloses application of the Echinocandin B high-producing strain. By using the obtained Echinocandin B high-producing strain, the yield of the prepared Echinocandin B reaches about 800 mg/l.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, specifically, an echinocandin B high-yield bacterial strain and its application. Background technique [0002] In the past two decades, fungal infections that seriously endanger human life and health have been increasing in terms of mortality and types of infections, especially in immunosuppressed patients. The main drugs currently used to treat clinical deep fungal infections are azole antifungal drugs and amphotericin B. [0003] Although these two types of drugs have played an important role in controlling clinical deep fungal infections, the mortality rate of deep fungal infections remains high due to the following three deficiencies of these drugs: [0004] First, because the targets of these two types of drugs are fungal cell membranes, their selectivity is poor, resulting in relatively large toxic and side effects; [0005] The second is the reduced efficacy of these drugs due to ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P21/04C12R1/66
Inventor 阮丽军孙新强杨天陈迎迎罗敏玉吕坚方夏兴张磊
Owner NOVOCODEX BIOPHARMACEUTICALS CO LTD
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