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Application of fructose as vaccine adjuvant

A vaccine adjuvant and vaccine technology, applied in the field of biomedicine, can solve problems such as adjuvant disease, and achieve the effect of not causing toxic and side effects

Inactive Publication Date: 2013-10-02
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The most classic vaccine adjuvant at present is Freund's adjuvant, but this adjuvant is mainly used in animal experiments, but adjuvant disease often occurs after repeated injections in animals

Method used

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  • Application of fructose as vaccine adjuvant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The cultivation and processing of embodiment 1 Edwardsiella tarda EIB202 bacterial strain

[0023] Pick the single clone of Edwardsiella tarda EIB202 on the Tryptone Soy Broth (TSB) plate in a TSB test tube, culture at 30°C for 24 hours, transfer to 100mL TSB medium at a ratio of 1:100 for expansion. When OD 600 When the value reached 1.0, the bacteria were collected by centrifugation, washed twice with normal saline, and then suspended with normal saline to make the OD 600 1.0 (the number of bacteria is about 10 9 bacteria / ml) for the challenge of experimental mice.

[0024] At the same time, another part of the bacteria was diluted 10 times with normal saline (about 10 8 cfu / mL) to obtain a live bacterial vaccine for the live bacterial immunization of experimental mice.

Embodiment 2

[0025] The immunoassay of embodiment 2 mice

[0026] Ninety 4-week-old Kunming mice (average body weight 25g) were randomly divided into 3 groups: live bacteria immunization group, live bacteria + fructose immunization group, and control group. Each mouse in the live bacteria immunization group was intraperitoneally injected with 100 μL of the prepared live bacteria vaccine (about 107 bacteria), and in the live bacteria + fructose immunization group, each mouse in the fructose added group was injected with the same live bacteria vaccine 100 μL and intraperitoneally. A mixture containing 10 μmol fructose. Each mouse in the control group was intraperitoneally injected with 100 μL of normal saline. Two consecutive immunizations with an interval of 7 days. Seven days after the last immunization, the mice were observed for 15 days, and the death of the mice was counted. The relative immune protection rate was calculated according to the formula (relative immune protection rate=[...

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Abstract

The invention relates to the field of biological medicines, and particularly discloses an application of fructose as a vaccine adjuvant. The feed added with the fructose has the advantage that the death rate of an experimental animal is remarkably reduced in comparison with a feed which is not added with alanine through adding or no adding the fructose in the vaccine, so that the fructose is proved to be capable of improving the immunity effect of the vaccine and can be used as the vaccine adjuvant.

Description

technical field [0001] The invention relates to the field of biomedicine, more specifically, relates to the application of fructose as a vaccine adjuvant. Background technique [0002] Fructose is the most common ketohexose. It exists in honey and fruit, and combines with glucose to form sucrose for daily consumption. Containing 6 carbon atoms in fructose, it is also a monosaccharide, which is an isomer of glucose. It exists in large quantities in fruit juice and honey in a free state. Fructose can also combine with glucose to form sucrose. Pure fructose is a colorless crystal with a melting point of 103-105°C. It is not easy to crystallize and is usually a viscous liquid that is easily soluble in water, ethanol and ether. Fructose is the sweetest monosaccharide. In the pharmaceutical industry, it is mainly used as pharmaceutical excipients and hangover products. [0003] Vaccine adjuvants are substances that are injected into the body together with the antigen or in adv...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61P37/04
Inventor 彭宣宪李惠王超
Owner SUN YAT SEN UNIV
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