Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction method and application of tumor exosome vaccine

A tumor exosome and extraction method technology, which is applied in the field of tumor exosome (exosome) vaccine extraction, can solve the problem of inability to extract miRNA antigens, and achieve the goal of improving tumor-specific immunogenicity and enhancing killing ability. Effect

Inactive Publication Date: 2013-10-09
ZHEJIANG UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The original method of extracting exosome only extracts the complete vesicle structure of exosome, but a large number of miRNA and tumor-associated antigens in the vesicle cannot be extracted

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method and application of tumor exosome vaccine
  • Extraction method and application of tumor exosome vaccine
  • Extraction method and application of tumor exosome vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Application of exosome in immunotherapy of cholangiocarcinoma cells

[0017] 1. Extract exosome: Fetal bovine serum was centrifuged at 100,000×g for 6 hours at 4°C to remove endogenous exosome. Human cholangiocarcinoma cells (RBE cells) were cultured in 1640 culture medium containing 10% fetal bovine serum for about After 72 hours, the medium supernatant was collected. Extract the exosome according to the Good Manufacturing Practice (GMP) standard: centrifuge the supernatant at 300×g for 5 minutes, transfer to a new centrifuge tube and centrifuge at 800×g for 30 minutes, transfer to a new centrifuge tube and centrifuge at 10,000×g for 30 minutes to remove cell debris; The supernatant was then centrifuged at 1,500×g for 30 minutes with a 100 kd ultrafiltration tube from Millipore Company; the concentrated solution was transferred to a 30 g / L sucrose heavy water pad, and ultracentrifuged at 100,000×g for 60 minutes. Take out the sucrose heavy water pad, dilute it into 50...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an extraction method and application of a tumor exosome vaccine. According to the invention, exosome is successfully extracted from the supernatant of a nutrient solution of human bile duct cancer cells, and an exosome lysis ultrafiltrate not containing miRNA is extracted by using an ultrafiltration lysis method. The exosome lysis ultrafiltrate not containing miRNA obtained in the invention can obviously stimulate immune cells to generate a specific tumor killing effect, greatly improve the killing effect, i.e., specific immune response, of the immune cells on a tumor, reduce the level of immune escape of tumor cells and enhance killing capability of the human immune cells on the tumor cells.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for extracting a human tumor vaccine, in particular to an extraction method and application of a tumor exosome vaccine. Background technique [0002] Surgical treatment is a routine treatment for tumors. In addition to surgical treatment, adoptive cellular immunotherapy is currently a very promising tumor treatment method. Compared with other treatment methods, adoptive cellular immunotherapy has the advantages of high specificity, less side effects, improvement of clinical signs, and prolongation of patient survival time. In vitro and animal experiments have shown that adoptive cells have a significant specific killing effect on tumor cells. [0003] The exosome is a small vesicle with a diameter of 50-100nm secreted by the fusion of the multivesicular bodies (MVBs) of many living cells with the cell membrane. Tumor cell-derived exosomes have been proven to express ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/16A61P35/00
Inventor 曹利平陈炯煌阙日升
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com