Bacterial culture solution for detecting pathogenic bacteria in blood

A technology for bacterial culture and pathogenic bacteria, which is applied in the determination/inspection of bacteria and microorganisms, biochemical equipment and methods, etc., can solve the problems of insufficient growth rate, shortened blood detection time, long detection time, etc., and achieve high accuracy, The effect of shortening the detection time and speed

Inactive Publication Date: 2013-10-09
史煜波
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the technology of detecting blood pathogenic bacteria, in order to solve the technical defect that the growth rate of aerobic bacteria in the blood is not fast enough, resulting in long detection time, the present invention discloses a A bacterial culture solution for detecting blood pathogenic bacteria, which can significantly increase the speed of bacterial reproduction, and can antagonize and neutralize antibiotics in blood, so that the speed of bacterial growth is fast and the detection time of blood is shortened; in addition, bacterial metabolites can By reacting with the bicarbonate in the culture medium and converting it into a chemical signal that can be recognized by the instrument, the accuracy of blood detection is high, so it has a wide application prospect in the field of blood clinical testing

Method used

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  • Bacterial culture solution for detecting pathogenic bacteria in blood
  • Bacterial culture solution for detecting pathogenic bacteria in blood
  • Bacterial culture solution for detecting pathogenic bacteria in blood

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Embodiment 1

[0014] Embodiment 1: The bacterial culture solution for detecting blood pathogenic bacteria of the present invention, its composition includes the nutrient substance needed for bacterial growth, anticoagulant and antagonistic antibiotic reagent, the mass concentration ratio of three kinds of compositions is 35:1:5 .

[0015] The nutrients required for the growth of the bacteria include one or more mixtures of peptone, tryptone, beef extract powder, glucose, vitamins and bicarbonate, and the mass concentration ratio thereof is 15:0.10:0.30.

[0016] The vitamins are composed of one or more mixed vitamins of vitamin A, vitamin D, vitamin E, B vitamins and vitamin E. Vitamins can be composed of one or more mixed in any proportion, as long as the culture solution contains vitamins.

[0017] The bicarbonate is composed of one or more of sodium bicarbonate, potassium bicarbonate, calcium bicarbonate and ammonium bicarbonate. Any one or any proportion of the selected bicarbonate ca...

Embodiment 2

[0027]Embodiment 2: The bacterial culture solution for detecting blood pathogenic bacteria of the present invention, its composition includes the nutrient substance needed for bacterial growth, anticoagulant and antagonistic antibiotic reagent, the mass concentration ratio of three kinds of compositions is 52:10:20 .

[0028] The nutrients required for the growth of the bacteria include one or more mixtures of peptone, tryptone, beef extract powder, glucose, vitamins and bicarbonate, and the mass concentration ratio thereof is 26:0.01:0.75.

[0029] The vitamins are composed of one or more mixed vitamins of vitamin A, vitamin D, vitamin E, B vitamins and vitamin E. Vitamins can be composed of one or more mixed in any proportion, as long as the culture solution contains vitamins.

[0030] The bicarbonate is composed of one or more of sodium bicarbonate, potassium bicarbonate, calcium bicarbonate and ammonium bicarbonate. Any one or any proportion of the selected bicarbonate c...

Embodiment 3

[0035] Embodiment 3: The bacterial culture solution for detecting blood pathogenic bacteria of the present invention, its composition comprises the nutrient substance needed for bacterial growth, anticoagulant and antagonistic antibiotic reagent, the mass concentration ratio of three kinds of compositions is 40:5:10 .

[0036] The nutrients required for the growth of the bacteria include one or more mixtures of peptone, tryptone, beef extract powder, glucose, vitamins and bicarbonate, and the mass concentration ratio thereof is 18:0.06:0.52.

[0037] The vitamins are composed of one or more mixed vitamins of vitamin A, vitamin D, vitamin E, B vitamins and vitamin E. Vitamins can be composed of one or more mixed in any proportion, as long as the culture solution contains vitamins.

[0038] The bicarbonate is composed of one or more of sodium bicarbonate, potassium bicarbonate, calcium bicarbonate and ammonium bicarbonate. Any one or any proportion of the selected bicarbonate ...

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Abstract

The invention discloses a bacterial culture solution for detecting pathogenic bacteria in blood. The bacterial culture solution comprises nutrient substances required by the growth of bacteria, an anticoagulant and an antagonistic antibiotic reagent according to the mass-concentration ratio being (35-52):(1-10):(5-20). Compared with the traditional culture medium cultured in blood microbial culture tools such as a blood bacterial enrichment broth and a manual dual-way aerobic hemoline, the bacterial culture solution is rapid in bacterial reproduction, so that the blood detection time is shortened; and in addition, metabolic products of the bacteria can react with bicarbonate in the culture solution so as to be converted to form chemical signals identified by an instrument, so that the blood detection accuracy is high. Thus, the bacterial culture solution has a wide application prospect in the field of clinical blood detection.

Description

technical field [0001] The invention belongs to the technical field of blood detection, in particular to a bacterial culture solution for detecting blood pathogenic bacteria. Background technique [0002] The automatic aerobic blood culture bottle is a new type of microbial culture system used in the field of medical clinical testing, and is a new generation of microbial detection reagents used in conjunction with automatic blood culture instruments. It consists of three parts: a bottle body, a solid sensor, and a culture medium. It can be used in combination with a fully automatic blood culture instrument to quickly detect whether bacteria, fungi, and mycobacteria are infected in blood or other specimens, so as to provide rapid and effective clinical anti-bacteria. Infection treatment provides the basis for diagnosis. The automatic blood culture system was first developed in the 1990s by BD Company of the United States and Mérieux Company of France. At present, it has been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/20
Inventor 史煜波
Owner 史煜波
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