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Diluent for hematology analyzer

A technology of blood cell analyzer and diluent, which is applied in the preparation of test samples, etc., can solve the problems of easy adsorption of residues in detection cells and pipelines, shortening the service life of detection cells and pipelines, and long time for cleaning instruments. , to achieve the effect of long storage time, preventing residues and preventing bacterial growth

Pending Publication Date: 2021-06-04
JINHUA XINKE PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the daily use of the hematology analyzer, it is inevitable that there will be minor faults, and the hole plugging is the most common problem. Because the protein components in the sample combine with the cationic surfactant in the hemolytic agent, the protein will be denatured. At the same time, the cationic surfactant The molecular structure contains a hydrophilic group, not only has no washing effect, but it is easy to make the residue easily adsorbed in the detection pool and pipeline. The diluent used for routine blood cell analysis has no surfactant, which is easy to cause the hole of the instrument to be blocked, and the blocked hole should be solved If there is a fault, the instrument detection pool and pipeline must be soaked with sodium hypochlorite cleaning solution. It takes a long time to clean the instrument and affects the detection efficiency. In addition, frequent use of sodium hypochlorite cleaning solution for a long time will shorten the service life of the detection pool and pipeline, and increase the use cost of the instrument.

Method used

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  • Diluent for hematology analyzer
  • Diluent for hematology analyzer
  • Diluent for hematology analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A diluent for blood cell analysis, containing 5.2g / L sodium chloride, 7.0g / L sodium sulfate, 5.3g / L disodium hydrogen phosphate, 0.75g / L potassium dihydrogen phosphate, 0.5g / L EDTA, 0.3g / L Proclin 300, 0.02g / L polyether, pH 7.0, after mixing and stirring the above components to dissolve to a constant volume, the diluent for hematology analyzer prepared in Example 1 was obtained.

[0016] Place the diluent for hematology analyzer prepared in Example 1 on the corresponding position of Mindray BC5180 hematology analyzer for verification test, take venous blood samples from 20 patients for whole blood mode measurement and dilute the blood samples with this diluent and let stand for 30 minutes Determination in post-dilution mode, compared with the background count, carryover rate, intra-assay precision, linear range, accuracy and comparability of diluents used for routine blood cell analysis.

[0017]

[0018]

[0019]

[0020] The results show that the self-prepare...

Embodiment 2

[0024] A diluent for blood cell analysis, containing 7.0g / L sodium chloride, 3.2g / L sodium sulfate, 5.3g / L disodium hydrogen phosphate, 0.75g / L potassium dihydrogen phosphate, 0.5g / L EDTA, 0.3g / L Proclin 300, 0.02g / L polyether, pH 7.0, after mixing and stirring the above components to dissolve to a constant volume, the diluent for hematology analyzer prepared in Example 2 was obtained.

[0025] Place the diluent for hematology analyzer prepared in Example 2 on the corresponding position of Mindray BC6800 hematology analyzer for verification test, take venous blood samples from 20 patients for whole blood mode measurement and dilute the blood samples with this diluent and let stand for 30 minutes Determination in post-dilution mode, compared with the background count, carryover rate, intra-assay precision, linear range, accuracy and comparability of diluents used for routine blood cell analysis.

[0026]

[0027]

[0028]

[0029] The results show that the diluent for ...

Embodiment 3

[0033] A diluent for blood cell analysis, containing 1.3g / L sodium chloride, 11.8g / L sodium sulfate, 2.0g / L borax, 1.5g / L boric acid, 0.5g / L EDTA, 0.4g / L L of Proclin 300, 0.02g / L polyether, pH 7.1, the above components were mixed and stirred to dissolve to a constant volume, and the diluent for blood cell analysis prepared in Example 2 was obtained.

[0034] Place the diluent for hematology analyzer prepared in Example 3 on the corresponding position of Mindray BC1800 hematology analyzer for verification test, take venous blood samples from 20 patients for whole blood mode measurement and dilute the blood samples with this diluent and let stand for 30 minutes Determination in post-dilution mode, compared with the background count, carryover rate, intra-assay precision, linear range, accuracy and comparability of diluents used for routine blood cell analysis.

[0035]

[0036]

[0037]

[0038] The results show that the self-prepared diluent for blood cell analysis in...

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Abstract

The invention discloses a diluent for a hematology analyzer. The diluent contains 1-10g / L of sodium chloride, 1-10g / L of sodium sulfate, 1-20g / L of a buffer agent, 0.1-1g / L of an antibacterial agent, 0.1-1.0 g / L of a complexing agent and 0.01-0.1 g / L of a low-foaming surfactant. The diluent is capable of preventing hemolysis, wherein the buffer agent has the effects of maintaining the pH value of a detection environment and enhancing the stability of a detection result, the antibacterial agent prevents the growth of bacteria in the diluent, and the preservation time is longer, and the complexing agent prevents the blood sample from coagulating in the diluent; besides, the low-foam surfactant improves the surface tension of a reaction system and prevents the blood sample and a detection reagent from remaining in a detection pool and a pipeline under the conditions that the blood sample is not hemolyzed in the diluent and no bubbles influencing the detection result are generated in the diluent adding process, so that the instrument is prevented from hole blocking.

Description

technical field [0001] The invention relates to the technical field of blood cell analysis, in particular to a diluent for a blood cell analyzer. Background technique [0002] In the current social development, driven by science and technology, hematology analyzers have become an indispensable part of hospital equipment. The accuracy of the numerical values ​​issued by hematology analyzers is very important for doctors and patients. [0003] In addition to providing measured cell data, the blood cell analyzer can also display various blood cell volume distribution graphics. These graphs that can represent the distribution of cell populations are called cell histograms. Hematology analyzers have been widely used in hospitals at all levels in our country. It not only improves the accuracy of the experimental results, but also provides many experimental indicators, which play an important role in the diagnosis and differential diagnosis of diseases. [0004] During the daily...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 方啟龙胡利民
Owner JINHUA XINKE PHARMA TECH CO LTD
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