Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo

A technology of bacillus cereus and liquid preparations, which is applied in the field of prevention and treatment of hybrid bamboo white spot feather disease, and can solve the problems of phytotoxicity and drug resistance

Inactive Publication Date: 2015-03-25
SICHUAN AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical control is an effective control method, there are many practical problems such as pathogen resistance, environment and food safety, and drug damage. Some chemical fungicides have been strictly restricted in many developed countries and regions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo
  • Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo
  • Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] The present invention will be described in detail below in conjunction with specific embodiments.

[0007] (1) Screening of Bacillus cereus B2 strain

[0008] 1.1 Pathogens tested

[0009] The pathogenic bacterium tested was the hybrid white-leafed feather fungus [Rosellinia necatrix (Hart.) Berl.].

[0010] 1.2 Isolation and purification of spores

[0011] Take the hybrid bamboo rhizosphere soil (below the surface layer 10cm) and put it into a sterile sample bag and bring it back to the laboratory for future use. Weigh 10 per sample g Transfer to a Erlenmeyer flask filled with 90 mL of normal saline and a small amount of glass beads, mix thoroughly to make a soil suspension, and heat in a 100°C water bath for 5 minutes. After cooling, take 1mL under aseptic conditions and add it to a test tube filled with 9mL sterile water, and mix well to obtain 10 -2 The sample diluents were diluted sequentially according to this method to obtain 10 -3 、10 -4 、10 -5 and 10 -...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses bacillus cereus strain B2, a liquid preparation, a preparation method of the liquid preparation and an application in controlling rosellinia necatrix of hybridized bamboo. The strain has been preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms (CGMCC), Chaoyang District, Beijing, China, since 20th June, 2013, and has a preservation number of CGMCC No.7767 and a classified name of bacillus cereus. The above liquid preparation is diluted by 100-200 times (volume ratio) and is poured to the root of each of non-pathogeny hybridized bamboos along root system breadth direction with 100 mL per plant and once in spring every year, thereby the generation of rosellinia necatrix can be prevented. The above liquid preparation is diluted by 50-100 times (volume ratio) and is poured to the root of each of morbidity hybridized bamboos along root system breadth direction with 200 mL per plant and once in spring and once in summer every year for three successive years, thereby rosellinia necatrix can be effectively cured.

Description

technical field [0001] The invention relates to a Bacillus cereus B2 strain, a liquid preparation and a preparation method thereof, and an application in preventing and treating hybrid bamboo white spot feather disease. Background technique [0002] Support × green hybrid bamboo is an important economic timber bamboo in the south, and it is also one of the main economic bamboo species introduced and developed in Sichuan and other places. In recent years, the white-striped feather disease of hybrid bamboo caused by Rosellinia necatrix (Hart.) Berl. has led to root rot and the death of whole bamboo plants, resulting in large economic losses and a great threat to the middle and upper reaches of the Yangtze River. The process of returning farmland to forests and the construction of ecological barriers. At present, chemical control and silviculture techniques are mainly used, while research on biological control measures has not yet been involved. Although chemical control is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/085
Inventor 朱天辉李姝江邵宝林张静韩珊谯天敏郑磊张丽娜
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products