35 results about "B cereus" patented technology

The utility model relates to the technical field of liquor fermentation, and discloses a culture device for bacillus cereus in Maotai-flavor yeast for making hard liquor, which comprises a tank body, a sampling tube is arranged at the bottom of the tank body, a sample storage barrel is fixed at the discharge end of the sampling tube and is communicated with the sampling tube, a partition plate for dividing an inner cavity into a cavity A and a cavity B is fixed in the sample storage barrel, and the sampling tube is communicated with the partition plate. An annular groove is formed in the position, away from the sampling pipe, of the inner wall of the sample storage barrel, a check ring is installed in the annular groove, a rotatable rubber plug is installed between the check ring and the partition plate, the rubber plug is tightly pressed on the partition plate through the check ring, a through hole B is formed in the rubber plug, and the through hole A is located on the rotating track of the through hole B; a square hole is further formed in the center of the rubber plug. The sampling device has the beneficial effects that a test tube is inserted into the through hole B for sampling, so that a culture solution is prevented from remaining in the through hole B, and the accuracy of a sampling detection result is improved.

The application provides a rhodamine bacterial lipid droplet membrane fluorescent dye and synthesis and application thereof. The fluorescent dye is based on the affinity of a long carbon chain connected to a nitrogen atom of rhodamine to lipids, the affinity of a positively charged open-loop rhodamine to a negative potential lipid membrane, and the enhancement of a benzene ring to TICT to reduce the background of non-lipid droplet membrane sites. A dye (C4, C6, C8) capable of positioning on a bacterial lipid droplet membrane is designed and synthesized, and the structural formula is shown in formula (1), wherein n is 2, 4 or 6. The dye realizes the lipid droplet membrane imaging of Bacillus cereus, Rhodococcus opacus , Rhodococcus jostii RHA1.

The invention discloses a method for preparing an organic fertilizer by utilizing defective grapefruit and application of the organic fertilizer, and belongs to the technical field of agricultural waste resource utilization and microbial fertilizers. The microbacterium A5, the bacillus flexus HL-37 and the bacillus cereus TH-35 are used for degrading the defective grapefruit fruits to prepare the organic bacterial fertilizer, the liquid bacterial fertilizer prepared through a staged aerobic fermentation process is mixed with soybean meal / manure according to the ratio of 1: 1 to be returned to a grapefruit orchard, the weight of the grapefruit fruits can be increased by 10.2%, the sugar content can be increased by 5.3%, meanwhile, odor is eliminated, and the pH value of alkaline soil is neutralized; when the solid organic fertilizer is used for vegetable planting, the yield is increased by 23.8% compared with that of a chemical fertilizer, and the microbial structure of soil is remarkably improved. The method realizes resource utilization of agricultural wastes, and has both environmental protection and yield increase benefits.

The present invention discloses a special water quality regulator for aquaculture and its preparation method, which relates to the technical field of aquaculture. The special water quality regulator for aquaculture of the present invention comprises the following raw material components in parts by mass: 8-15 parts of compound microorganisms, 35-80 parts of clay ore, 15-20 parts of water extract of sunflower leaves, 8-12 parts of water buttercup extract, 2-3 parts of picroside II, 5-6 parts of glucose, and 0.02-0.04 parts of ferrous chloride; the compound microorganisms are obtained by compounding bacillus cereus and acinetobacter johnsonii in a mass ratio of 1-2:1. The preparation method of the special water quality regulator for aquaculture of the present invention is simple, the raw materials are easy to obtain, and the purification effect of the aquaculture water body is excellent, having an important application prospect in the purification of aquaculture water bodies.

The invention discloses a bacillus cereus strain and an application thereof. The technical problem to be solved by the invention is how to degrade phosphate fertilizer which is difficult to absorb in soil. The strain number of the bacillus cereus is LCWJL1997, and the registration number of the bacillus cereus in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms is CGMCC (China General Microbiological Culture Collection Center) No. 29630. Experiments prove that the bacillus cereus LCWJL1997 has the function of dissolving phosphorus. The strain is used for preparing a fertilizer, and corn plant and soil indexes are analyzed through a corn pot experiment, so that the strain not only can improve the soil environment, but also can promote crop growth and play a role in reducing the dosage of a chemical phosphate fertilizer, and has important significance in improving the ecological environment.

The invention discloses a bacillus cereus strain and application thereof, a phylogenetic tree is constructed through whole genome sequencing according to housekeeping genes, the phylogenetic tree is determined to belong to bacillus cereus (Bacillus cereus), and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34800. The strain B2 obtained through separation can secrete indoleacetic acid (IAA), IAA serves as auxin and is beneficial for stimulating plant root development and promoting root growth, so that the water and nutrient absorption capacity of plants is enhanced, the adaptability of the plants in a stress environment is improved, meanwhile, the strain B2 can regulate and control sodium and potassium ion transport in pepper plants, and the yield of the pepper plants is increased. Specifically, the expression level of the key ion transporter gene in the pepper is up-regulated. The bacillus cereus strain B2 is an efficient microbial resource and can be used for developing a microbial inoculant or a biological fertilizer so as to promote growth of crops such as capsicum in salinized soil, improve the salt resistance of the crops and guarantee and improve the sustainable production capacity of capsicum in the salinized soil.

The utility model belongs to the technical field of coating, and particularly relates to a nozzle-adjustable coating device for bacillus cereus, which comprises a coating main body, the top end of the coating main body is fixedly connected with a stirrer, the output end of the stirrer is fixedly connected with a rotating shaft, the outer wall of the rotating shaft is fixedly connected with a stirring rod, and the stirring rod is fixedly connected with a nozzle. The bottom end of the rotating shaft is fixedly connected with a material turning plate, the side wall of the coating main body is fixedly connected with a feeding port, one side, located on the side wall of the coating main body, of the feeding port is fixedly connected with a material adding port, and the inner wall of the coating main body is fixedly connected with a fixing ring. The threaded rod rotates to drive the lifting ring to vertically slide along the inner wall of the coating body through threads, the lifting ring drives the four sets of lifting plates to synchronously slide, the lifting plates slide to drive the adjusting plates to rotate with the fixing ring as the circle center through the adjusting guide grooves, and the adjusting plates rotate to drive the coating nozzles to synchronously rotate. Further, the mixed materials in the coating main body are comprehensively coated.

The present application is a method for effectively inactivating spores in Bacillus by using chelating resin in the field of sterilization. The method uses chelating resin to inactivate spores in Bacillus. Specifically, chelating resin is used to quickly and effectively inactivate spores in Bacillus cereus, Bacillus safensis, Bacillus albus, and Bacillus subtilis. The chelating resin used has low production cost and can be regenerated and reused, achieving effective inactivation of spores in a simple, low-cost, and environmentally friendly manner.

The application discloses application of barley malt alkali in inhibition of growth and pathogenicity of Bacillus cereus by regulating a quorum sensing system of the Bacillus cereus. The barley malt alkali is a green natural product, is safer for the environment and human bodies than traditional chemical antibacterial agents such as antibiotics, and reduces the risk of drug residue and drug-resistant strain generation. Research results show that the barley malt alkali can play a role at a low concentration (1 / 8 MIC), so that the use cost is low. The application can target regulation of expression of multiple key genes (such as Spo0A, CodY, rpoN and the like), so as to not only inhibit pathogenicity, but also effectively control proliferation of the Bacillus cereus, reduce the risk of food contamination and foodborne disease infection, and has important application prospects in the field of food safety.

The application relates to a bacillus cereus for antagonizing multiple pathogenic fungi of potatoes and an application thereof, and relates to the field of biological fungicides.The fungicide is bacillus cereus Bc19, which is preserved in the China General Microbiological Culture Collection Center, the address of which is No. 3, Beichen West Road, Chaoyang District, Beijing, and the preservation date is November 11, 2024, and the preservation number is CGMCC No. 32578.The application is used for preparing the fungicide for antagonizing multiple pathogenic fungi of potatoes.A bacillus cereus Bc19 strain with inhibiting effects on six pathogenic fungi of potatoes is obtained, high-quality strains for developing and preparing the fungicide for widely inhibiting pathogenic fungi of soil-borne diseases of potatoes are provided, and a foundation is laid.

This invention discloses a microbial compound inoculant and its application in blueberry growth, belonging to the field of microbial inoculant technology. The compound inoculant is composed of *Bacillus cereus*, *Pseudomonas koreensis*, and *Rhodococcus qingshengii* in a viable count-to-mass ratio of 1:1:1. The three strains exhibit significant synergistic effects, not only improving growth indicators such as stem length, root length, fresh weight, and dry weight of blueberries, but also increasing the content of soluble sugars, soluble proteins, and proline in blueberry leaves. The microbial compound inoculant constructed in this invention is adapted to the acidic growth environment of blueberries, is environmentally friendly, and low in cost, solving the problems of fertilizer dependence, slow growth, and lack of specialized inoculants in blueberry cultivation, and providing technical support for large-scale artificial cultivation of blueberries.

The application discloses a non-natural base DNA aptamer targeting Bacillus cereus 5 / B / 6 metal beta-lactamase and a screening method thereof. The screening method uses dNaMTP and dTPT3TP, or dCNMOTP and dTPT3TP as non-natural base nucleoside triphosphate substrates, uses OneTaq DNA polymerase to amplify a DNA library containing non-natural bases, and uses lamda exonuclease to degrade 5' end phosphorylated DNA chains in PCR products, so as to obtain two ssDNA libraries. The non-natural base DNA aptamer targeting Bacillus cereus 5 / B / 6 metal beta-lactamase is screened based on the libraries, and three non-natural base DNA aptamers with a length of 70 nt are obtained. The results of a biological membrane layer interference analysis show that the three non-natural base DNA aptamers have good binding affinity to Bacillus cereus 5 / B / 6 metal beta-lactamase.

The invention belongs to the technical field of tobacco processing and biological detection, and relates to a paper-based lateral flow detection strip for bacillus cereus residues in tobacco shreds and a preparation method, the detection strip comprises a back plate, and a sample pad, a conjugate pad, a reaction film and an absorption pad which are sequentially arranged on the back plate along a liquid flowing direction; the conjugate pad is loaded with a BAS-6F aptamer probe marked by gold nanoparticles; a test line and a control line are sequentially arranged on the reaction film in the liquid flowing direction, a BAS-6R aptamer is fixed on the test line, and streptavidin is fixed on the control line; the nucleotide sequence of the BAS-6F aptamer is as shown in SEQ ID NO. 1; the nucleotide sequence of the BAS-6R aptamer is as shown in SEQ ID NO. 2; the invention provides a rapid tool specially used for detecting tolerance spores in'sterilized tobacco shreds after fermentation and cut tobacco drying ', and fills the blank of rapid detection technology in the scene.

The present invention teaches a bioassay module for detection of pathogenic microorganisms comprising: food specimen deposit element; indicator layer comprising dye; enzymatically digestible substrate layer, forming a barrier between the food specimen deposit element and indicator layer; isolation layer, separating the enzymatically digestible substrate layer from layers underlying the enzymatically digestible substrate layer; separation layer; absorption layer to absorb and display the indicator; wherein the layers are disposed from bottom to top the absorption layer, the separation layer, the indicator layer, the isolation layer, the enzymatically digestible substrate layer, the food specimen deposit element, and enzymatically digestible substrate layer is liquefiable and perforable by enzymes secreted by the pathogenic microorganism in contaminated food disposed in the food specimen deposit element, the digested substrate layer is flowable to the indicator layer via the isolating layer, the liquefied dye flowable to the absorption layer and visible at least from the top layer.

The application belongs to the technical field of industrial circulating water treatment, and provides a green water treatment agent with corrosion inhibition and bacteriostasis performance, a preparation method and application, the water treatment agent has the structure as shown: The application introduces a piperazine group into a polyaspartic acid peptide bond structure by a graft copolymerization method, synthesizes a novel water treatment agent, the water treatment agent can be adsorbed on a metal surface to form a protective film, has good corrosion inhibition and bacteriostasis performance. The water treatment agent also has bacteriostasis specificity, can inhibit escherichia coli, and retains bacillus cereus. Therefore, the application realizes efficient improvement of the corrosion inhibition performance and bacteriostasis performance of polyaspartic acid, reduces the application of phosphorus-containing compounds, and avoids the influence on the environment.

The invention discloses a method, a primer pair and a kit for detecting bacillus cereus, and belongs to the field of microbiological detection. The nucleotide sequences of the primer pair are respectively as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The detection method comprises the following steps: (1) extracting genome DNA (deoxyribonucleic acid) of a sample to be detected as a template, and performing PCR (polymerase chain reaction) amplification by taking primer pairs with nucleotide sequences as shown in SEQ ID NO.1 and SEQ ID NO.2 as primers; and (2) detecting whether a single amplification product exists at the 231 bp position in the PCR amplification product. The detection method disclosed by the invention is short in detection time, low in detection cost and reliable in detection result, and can be applied to detection of bacillus cereus in food.

The invention belongs to the technical field of environmental functional materials, and discloses a composite electrode material for selective lead removal and a preparation method and application thereof. The preparation method of the composite electrode material for selective lead removal comprises the following steps: culturing and collecting bacillus cereus, sequentially adding CoCl2. 6H2O and NaBH4 into a thallus suspension, reacting, centrifuging, washing, drying and calcining to obtain the Co3O4-x and thallus derived carbon composite electrode material. The composite electrode material is combined with double electric layers and a Faraday storage mechanism, the Pb < 2 + > adsorption capacity is 60-120 mg / g, the adsorption rate is 2.0-4.0 mg / g / min, the performance is remarkably superior to that of a traditional activated carbon electrode, and the key problems that cobalt-based oxide is poor in conductivity, poor in selectivity and insufficient in cycling stability in capacitive deionization application are effectively solved.

The invention discloses a bacillus cereus strain for producing tetramethylpyrazine and application of the bacillus cereus strain. The name of the bacillus cereus is bacillus cereus A1030, the preservation number of the bacillus cereus is GDMCC No: 66145, and the bacillus cereus is preserved in Guangdong Microbial Culture Collection Center on April 14, 2025. The bacillus cereus has very strong high temperature (50C) resistant and ethanol (20%) resistant growth capability; the bacillus cereus produces protease and amylase, the HC value of the amylase transparent circle is 2.19, and the HC value of the protease transparent circle is 1.7; the liquid state fermentation of the bacillus cereus can produce tetramethylpyrazine, and the content of tetramethylpyrazine is 0.8 g / L.

The utility model relates to composite culture jar technical field especially relates to bacillus cereus composite culture jar, including support platform and transparent incubation jar, the upper end of support platform is equipped with the transparent incubation jar for cultivating bacillus cereus and being convenient for observation.

The utility model is applicable to the technical field of bacillus cereus fermentation, and provides bacillus cereus solid-state fermentation equipment, which comprises an aluminum temperature adjusting tank, a fermentation tank, a fermentation tank and a fermentation tank, wherein a circular opening is formed in the top of the aluminum temperature adjusting tank; the fermentation tank is arranged on the circular opening, is used for accommodating bacillus cereus and is provided with a sleeve plate; the bottom plate is positioned at the bottom of the aluminum temperature adjusting tank and is used for supporting the fermentation equipment; the support column is fixedly arranged on the bottom plate and is used for supporting the aluminum temperature adjusting tank; the cover plate is positioned at the top of the sleeve plate and is used for sealing the fermentation tank; and the fermentation temperature control mechanism is arranged on the aluminum temperature adjusting tank and is used for adjusting and controlling the fermentation temperature. The bacillus cereus solid-state fermentation equipment provided by the scheme not only can ferment bacillus cereus, but also can control the fermentation temperature, has an auxiliary ventilation function, and is beneficial to rapid fermentation of the bacillus cereus.

The invention provides application of bacillus cereus and metabolites thereof in gastric cancer treatment, and relates to the field of disease treatment. The invention finds that BC can realize mutual assistance metabolism through a metabolite L-threonine, so that the abundance of AM is recovered. The BC and the metabolic product L-threonine of the BC can enhance the antagonism of the AM-NEDD4 axis on CAD-mediated ferroptosis defense, so that the tumor inhibition effect of AM combined anti-PD-1 treatment is synergistically improved in a gastric cancer model. In conclusion, the invention reveals the unclarified association among BC, AM and gastric cancer before. The AM plays a key role in regulating ferroptosis, shaping immune response and influencing the anti-PD-1 treatment effect. The AM-driven ferroptosis program is enhanced, and anti-PD-1 treatment is combined, so that the medicine is expected to become a new strategy for treating gastric cancer.

The present invention relates to a fusion protein having a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member and an enzyme having serine protease activity, wherein the enzyme having serine protease activity is from Bacillus firmus or is a variant of such enzyme. The present invention also provides a recombinant Bacillus cereus family member that expresses such fusion protein and exosporium fragments derived from such recombinant Bacillus cereus family member. Methods of using such recombinant Bacillus cereus family members or exosporium fragments derived therefrom for nematode control are also provided.

The preparation method of the bacillus cereus water dispersible granules disclosed by the invention specifically comprises the following steps: step 1, inoculating bacillus cereus on a nutrient agar plate culture medium, and culturing to obtain an activated strain; 2, picking single colonies from the activated strains, inoculating the single colonies to a seed culture medium, and culturing to obtain a seed solution; step 3, inoculating the seed solution into the fermentation culture medium, and performing fermentation culture to obtain a fermentation solution; step 4, performing spray drying on the fermentation liquor to obtain bacillus cereus powder; step 5, mixing the bacillus cereus powder obtained in the step 4 with a dispersing agent, a wetting agent, a disintegrating agent, a binder and a carrier; step 6, putting the mixture obtained in the step 5 into a jet mill, and crushing to obtain raw material powder; and step 7, adding deionized water into the raw material powder, uniformly mixing and kneading, extruding and granulating through a screen, and drying to obtain the bacillus cereus water dispersible granules. The invention further discloses the bacillus cereus water dispersible granules obtained by the method and application of the bacillus cereus water dispersible granules in prevention and treatment of kiwifruit canker.

The application discloses a method for preparing organic fertilizer by using pomelo residual and defective fruits and application thereof, and belongs to the technical field of agricultural waste resource utilization and microbial fertilizer. The method utilizes microbacterium A5, bacillus flexus HL-37 and bacillus cereus TH-35 to degrade pomelo residual and defective fruits, and prepares organic microbial fertilizer. Through a segmented aerobic fermentation process, the prepared liquid microbial fertilizer is mixed with soybean meal / fecal fertilizer at a ratio of 1:1 to be applied to a pomelo orchard, so that the weight of pomelo fruits is increased by 10.2%, the sugar content is increased by 5.3%, the odor is eliminated, and the alkaline soil pH value is neutralized. When the solid organic fertilizer is used for vegetable planting, the yield is increased by 23.8% compared with chemical fertilizer, and the soil microbial structure is significantly improved. The application realizes the resource utilization of agricultural wastes, and has the environmental protection and yield increasing benefits.

The application relates to a pair of antigen binding proteins specifically combined with Bacillus cereus NheA, and relates to the field of biological immunization technology. The application obtains a pair of antigen binding proteins specifically combined with Bacillus cereus NheA through screening, and the pair of antigen binding proteins are named as 5C1 and 8G7 and have unique variable region sequences. The non-hemolytic enterotoxin Nhe A of Bacillus cereus is detected by using a double-antibody sandwich detection method with 5C1 as a capture antibody and 8G7 as a detection antibody, and the minimum detection limit is 0.8 ng / mL, which indicates that the recombinant antibody has good sensitivity to Nhe A. In addition, the recombinant antibody has no cross reaction to other microbial toxins and has good specificity.

The invention discloses a method for detecting vomitoxin produced by bacillus cereus in raw milk or fermented feed, and relates to the technical field of quality safety detection of raw milk and feed. According to the method provided by the invention, high-accuracy and high-sensitivity detection of the vomitoxin in the raw milk or the fermented feed can be realized, and rapid, accurate and quantitative detection requirements can be met. Raw milk or a fermented feed sample is pretreated through a Captiva EMR-GPD purification column, interference of protein macromolecular substances and polar and non-polar small molecular substances such as pigments and sugar on a target object is effectively removed, the matrix effect caused by impurities in the mass spectrometric detection process is reduced, and the detection sensitivity and accuracy can be greatly improved. The method provided by the invention is strong in specificity, high in qualitative and quantitative detection accuracy and good in sensitivity, and has important significance for guaranteeing the safety of raw milk and feed.

This invention discloses an antimicrobial peptide, sesquimycin, and its applications. Specifically, it involves eight sesquimycin-like polypeptide compounds. Compounds 1-8 exhibit varying degrees of antimicrobial activity, with compound 2 possessing broad-spectrum antimicrobial activity. Its minimum inhibitory concentration (MIC) against *Staphylococcus aureus* ATCC 6538 and *Bacillus cereus* CMCC(B)63301 reaches 20 μg / mL, and its MIC against *Enterococcus faecalis* 160119481, *Salmonella typhi* CMCC(B)50071, and *Salmonella typhimurium* CMCC(B)50115 is 40 μg / mL. Given that *Staphylococcus*, *Bacillus*, *Enterococcus*, and *Salmonella* are common foodborne pathogens posing a serious threat to food safety, compounds 1-8 can be used to prepare novel antimicrobial peptides for application in the field of food safety preservation.

The invention provides a strain for producing extracellular polysaccharide with lipid-lowering activity and a method for preparing the extracellular polysaccharide with lipid-lowering activity by using the strain. The strain is bacillus cereus (Bacillus cereus) SGAG1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34999, the preservation date is June 24, 2025, the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postcode is 100101, and the telephone number is 010-64807355. The exopolysaccharide produced by the strain can up-regulate the expression of TFEB protein, and has the activity of reducing intracellular lipid accumulation.

The invention provides a microbial manganese oxide as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, activating bacillus cereus CY-1 and then culturing; step 2, adding a manganese source into the R2A culture medium, culturing, centrifuging and resuspending to obtain a microbial manganese oxide; the prepared microbial manganese oxide is suitable for efficient removal of organic pollutants in printing and dyeing wastewater, efficient targeted degradation of crystal violet is achieved through the synergistic degradation effect of the microbial manganese oxide and persulfate, and experiments show that the degradation rate can reach 86.0% within 60 minutes under the condition of the initial concentration of 20 mg / L CV, and the degradation rate can reach 86.0% within 60 minutes. Compared with the traditional single PMS degradation or pure chemical manganese oxide degradation, the efficiency is improved by 2-5 times.