4 results about "B cereus" patented technology

This invention discloses a microbial compound inoculant and its application in blueberry growth, belonging to the field of microbial inoculant technology. The compound inoculant is composed of *Bacillus cereus*, *Pseudomonas koreensis*, and *Rhodococcus qingshengii* in a viable count-to-mass ratio of 1:1:1. The three strains exhibit significant synergistic effects, not only improving growth indicators such as stem length, root length, fresh weight, and dry weight of blueberries, but also increasing the content of soluble sugars, soluble proteins, and proline in blueberry leaves. The microbial compound inoculant constructed in this invention is adapted to the acidic growth environment of blueberries, is environmentally friendly, and low in cost, solving the problems of fertilizer dependence, slow growth, and lack of specialized inoculants in blueberry cultivation, and providing technical support for large-scale artificial cultivation of blueberries.

The application discloses a non-natural base DNA aptamer targeting Bacillus cereus 5 / B / 6 metal beta-lactamase and a screening method thereof. The screening method uses dNaMTP and dTPT3TP, or dCNMOTP and dTPT3TP as non-natural base nucleoside triphosphate substrates, uses OneTaq DNA polymerase to amplify a DNA library containing non-natural bases, and uses lamda exonuclease to degrade 5' end phosphorylated DNA chains in PCR products, so as to obtain two ssDNA libraries. The non-natural base DNA aptamer targeting Bacillus cereus 5 / B / 6 metal beta-lactamase is screened based on the libraries, and three non-natural base DNA aptamers with a length of 70 nt are obtained. The results of a biological membrane layer interference analysis show that the three non-natural base DNA aptamers have good binding affinity to Bacillus cereus 5 / B / 6 metal beta-lactamase.

The utility model relates to composite culture jar technical field especially relates to bacillus cereus composite culture jar, including support platform and transparent incubation jar, the upper end of support platform is equipped with the transparent incubation jar for cultivating bacillus cereus and being convenient for observation.

The application relates to a pair of antigen binding proteins specifically combined with Bacillus cereus NheA, and relates to the field of biological immunization technology. The application obtains a pair of antigen binding proteins specifically combined with Bacillus cereus NheA through screening, and the pair of antigen binding proteins are named as 5C1 and 8G7 and have unique variable region sequences. The non-hemolytic enterotoxin Nhe A of Bacillus cereus is detected by using a double-antibody sandwich detection method with 5C1 as a capture antibody and 8G7 as a detection antibody, and the minimum detection limit is 0.8 ng / mL, which indicates that the recombinant antibody has good sensitivity to Nhe A. In addition, the recombinant antibody has no cross reaction to other microbial toxins and has good specificity.