50 results about "B cereus" patented technology

The invention belongs to the field of feed, and in particular relates to a laying hen feed for producing high-calcium and high-zinc nutritious eggs and a preparation method thereof. The laying hen feed contains: base material fermented product, calcium lactate, zinc gluconate, glycyl glutamine and high nucleic acid yeast hydrolyzate; Bacillus and the product after ventilated fermentation; the inoculation amount of said Bacillus cereus is 0.05-0.8% by weight, the inoculum amount of Bacillus megaterium is 0.005-0.05% by weight; the content of calcium lactate is 8-15% by weight, glucose The content of zinc acid zinc is 0.1-0.5% by weight, the content of glycyl glutamine is 0.01-0.5% by weight, and the content of high nucleic acid yeast hydrolyzate is 0.1-0.5% by weight. The laying hen feed of the invention obviously improves the laying quality of laying hens, and reduces the amount of feed to be fed, thereby improving the production benefits of laying hen breeding.

The invention discloses a nucleic acid thermostatic microfluidic chip sensor with double aptamer function for microorganism detection. The sample solution, magnetic capture complex-dumbbell-shaped probe, RCA-luminescent system, and buffer solution are added dropwise to the microfluidic chip, and the separation, enrichment and Detection; the aptamer of Bacillus cereus is obtained through whole-cell screening and tailoring, and has high affinity and specificity. At the same time, the sensor has the advantages of complete functions, simple and portable, can complete the detection within 1 hour, and does not need to be expanded and cultivated.

The invention belongs to the field of the detection of microorganisms and discloses a method for rapidly and accurately detecting live waxy bacilli capable of producing vomitoxin and waxy bacilli incapable of producing vomitoxin simultaneously. A specific primer is designed aiming to (i)cesB( / i) of waxy bacilli encoded vomitoxin and (i)16SrRNA( / i) of waxy bacilli; the interference of dead waxy bacilli in foods is removed by using propidium monoazide (PMA); the DNA of live bacilli in foods is extracted through a boiling water bath method; polymerase chain reaction (PCR) detection is repeatedly carried out; a possible false negative result in a PCR system is indicated through the addition of non-competitive internal amplification control (IAC). By adopting the method, the interference of normal false positive and negative results in the detection through a molecular biological method is avoided and then detection result is more accurate; meanwhile, the detection time of the method is shorter than that of a traditional culture method.