48 results about "B cereus" patented technology

The present invention relates to a kind of streptokinase, and is especially one kind of Bacillus cereus produced fibrinolysin, or ceryl kinase. The ceryl kinase has HPLC fingerprint with peak within 7-9 min in the conditions of chromatographic column Waters Protein -PakTM 60 7.8*300 mm, mobile phase 0.2mol / L NaH2PO4-CH3OH(95:5), flow rate 1ml / min and detecting wavelength 220 nm. The present invention also provides the preparation process of the ceryl kinase, and the use of the Bacillus cereus and the ceryl kinase in preparing thrombus treating medicine. The ceryl kinase of the present invention, the rat carotid artery thrombosis experiment shows, possesses obvious thrombosis resisting effect, so that the present invention provides one new option for treatment of thrombus diseases.

The invention discloses a saccharomyces cerevisiae engineering strain with high yield of 2'-fucosyllactose. Through metabolic engineering modification, the saccharomyces cerevisiae can absorb lactoseand takes GDP-mannose as a precursor in a cell to synthesize GDP-fucose, alpha-1,2-fucosyltransferase derived from bacillus cereus is subjected to heterologous expression, the obtained recombinant strain can produce the 2'-fucosyllactose at a high yield, and an important reference is provided for industrial production of the 2'-fucosyllactose.

The invention relates to the technical field of microbial fermentation, and discloses a method for fermenting a liquid fertilizer by using beneficial microorganisms. The method comprises the following steps: A, weighing the following raw materials in parts by mass: 11-14 parts of traditional Chinese medicine residues, 120-320 parts of a microbial fermentation agent, 2-8 parts of a nutritional agent, 50-90 parts of water, 35-45 parts of a solvent, 1-4 parts of a stabilizer, 3-9 parts of an additive, 16-24 parts of soybean meal, 1.5-2.5 parts of amino acid, 5-10 parts of rice bran and 3-8 parts of molasses, B, pouring the soybean meal, the rice bran, the amino acid and the traditional Chinese medicine residues into a container, uniformly stirring, adding water, and controlling the water content to be 62-68%. The microbial fermentation agent and the nutritional agent are added into the raw materials, balanced nutrients are provided for plants or crops, the raw materials are easy to obtain, harmless to soil and easy to decompose, and the bacillus cereus, the bacillus subtilis, the lactobacillus, the lactic acid coccus, the streptococcus faecalis and the bifidobacterium can degrade heavy metals such as lead, cadmium, mercury and arsenic, so that the soil activity is effectively improved; and the crop yield is improved.

The invention belongs to the field of agricultural science and technology, and particularly discloses a planting method of rice rich in selenium and SOD. The planting method comprises the steps of treating seeds, raising seedlings, performing transplant rice seedlings, performing fertilizing, performing field management, performing controlling of diseases and insect pests and performing harvesting, wherein in the process of planting rice, a reinforcing agent is applied, and the reinforcing agent comprises bacillus cereus capable of generating SOD and selenium-containing fertilizers. Accordingto the planting method, the content of SOD and the content of trace element selenium in rice grains can be increased at the same time, and the method is simple and convenient.

The invention belongs to the technical field of enzyme application, and in particular relates to an application of Bacillus cereus Y‑15 keratinase. The key protection of the present invention is the application of Bacillus cereus Y-15 keratinase in the preparation of cleaning products, especially the application of the keratinase in the preparation of washing products. The keratinase of the present invention is obtained by inoculating B. cereus Y-15 seed liquid into an optimized enzyme-producing medium and culturing it; it has excellent thermal stability and meets the following requirements: the half-life of the enzyme activity at 40°C is 34-36.5 h; the enzyme half-life t1 / 2 at 60°C is 5.5-6h, the half-life at 65°C is 2.2-2.5h, and the half-life at 70°C is 0.58-0.65h. In addition, the keratinase of the invention can tolerate reducing agents, oxidizing agents and detergents, and has potential application value in industrial production.

The invention discloses a preparation for improving an intestinal microbial environment. The preparation is prepared from the following main raw materials in parts by weight: 3-9 parts of monkshood, 3-10 parts of jasmine, 9-18 parts of fortune eupatorium herb, 4-12 parts of massa medicata fermentata fujianensis, 3-10 parts of rhizoma nelumbinis, 5-12 parts of concha ostreae, 9-20 parts of notoginseng powder, 5-12 parts of fructus amomi and 0.3-1.0 part of bacillus cereus. The selected medicinal raw materials of the preparation are traditional Chinese medicinal materials, have no side effect, are easily available and low in cost and the preparation is prepared on the basis of research of a lot of published documents, pharmacological work and clinical tests, and has a good curative effect on diarrhea and constipation caused by a chaotic intestinal microbial environment.

The invention provides application of bacillus cereus B-28 in the aspect of repairing the environment polluted by heavy metal Cr. The content of ammonium nitrate extraction state Cr and DTPA extraction state Cr of plant rhizosphere can be remarkably reduced by inoculating a B-28 strain to a plant, the content of Cr in each tissue of the plant is remarkably reduced, the chlorophyll content in plant leaves is promoted to be increased, the activity of CAT and POD enzymes at the root is promoted to be enhanced, the biomass of each tissue of the plant is remarkably improved, and a new scheme for plant-microorganism combined passivation of Cr to promote plant growth is provided.

The invention discloses bacillus cereus strain B2, a liquid preparation, a preparation method of the liquid preparation and an application in controlling rosellinia necatrix of hybridized bamboo. The strain has been preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms (CGMCC), Chaoyang District, Beijing, China, since 20th June, 2013, and has a preservation number of CGMCC No.7767 and a classified name of bacillus cereus. The above liquid preparation is diluted by 100-200 times (volume ratio) and is poured to the root of each of non-pathogeny hybridized bamboos along root system breadth direction with 100 mL per plant and once in spring every year, thereby the generation of rosellinia necatrix can be prevented. The above liquid preparation is diluted by 50-100 times (volume ratio) and is poured to the root of each of morbidity hybridized bamboos along root system breadth direction with 200 mL per plant and once in spring and once in summer every year for three successive years, thereby rosellinia necatrix can be effectively cured.

The invention belongs to the field of feed, and in particular relates to a laying hen feed for producing high-calcium and high-zinc nutritious eggs and a preparation method thereof. The laying hen feed contains: base material fermented product, calcium lactate, zinc gluconate, glycyl glutamine and high nucleic acid yeast hydrolyzate; Bacillus and the product after ventilated fermentation; the inoculation amount of said Bacillus cereus is 0.05-0.8% by weight, the inoculum amount of Bacillus megaterium is 0.005-0.05% by weight; the content of calcium lactate is 8-15% by weight, glucose The content of zinc acid zinc is 0.1-0.5% by weight, the content of glycyl glutamine is 0.01-0.5% by weight, and the content of high nucleic acid yeast hydrolyzate is 0.1-0.5% by weight. The laying hen feed of the invention obviously improves the laying quality of laying hens, and reduces the amount of feed to be fed, thereby improving the production benefits of laying hen breeding.

The invention discloses a nucleic acid thermostatic microfluidic chip sensor with double aptamer function for microorganism detection. The sample solution, magnetic capture complex-dumbbell-shaped probe, RCA-luminescent system, and buffer solution are added dropwise to the microfluidic chip, and the separation, enrichment and Detection; the aptamer of Bacillus cereus is obtained through whole-cell screening and tailoring, and has high affinity and specificity. At the same time, the sensor has the advantages of complete functions, simple and portable, can complete the detection within 1 hour, and does not need to be expanded and cultivated.

The invention provides a cultivation method of SOD-rich cereal crops. The cultivation method of the SOD-rich cereal crops of the present invention comprises: applying SOD-producing Bacillus cereus to the cereal crops. Compared with the traditional cultivation method, by directly applying the SOD-producing Bacillus cereus to the cereal crops, the present invention surprisingly realizes the increase of the SOD content of the cereal crops by several times, and the yield is also significantly increased.

The invention belongs to the field of the detection of microorganisms and discloses a method for rapidly and accurately detecting live waxy bacilli capable of producing vomitoxin and waxy bacilli incapable of producing vomitoxin simultaneously. A specific primer is designed aiming to (i)cesB( / i) of waxy bacilli encoded vomitoxin and (i)16SrRNA( / i) of waxy bacilli; the interference of dead waxy bacilli in foods is removed by using propidium monoazide (PMA); the DNA of live bacilli in foods is extracted through a boiling water bath method; polymerase chain reaction (PCR) detection is repeatedly carried out; a possible false negative result in a PCR system is indicated through the addition of non-competitive internal amplification control (IAC). By adopting the method, the interference of normal false positive and negative results in the detection through a molecular biological method is avoided and then detection result is more accurate; meanwhile, the detection time of the method is shorter than that of a traditional culture method.

The invention relates to the field of biotechnology, and concretely discloses a detection kit as well as a detection method and application for bacillus cereus. The detection kit is used for detecting the bacillus cereus through real-time fluorescence RPA, and comprises a pair of primers based on a bacillus cereus 16sRNA gene sequence and a probe. The detection method has excellent specificity and sensitivity, the detection limit is 1.0 x 10<-3>ng / muL, and when the pollution load is larger than or equal to 1.5 x 10<4>CFU / g, the bacillus cereus can be detected through the real-time fluorescence RPA, and only 6 to 13min is required. Therefore, the real-time fluorescence RPA established by the invention can be used for quickly, efficiently and sensitively detecting the bacillus cereus, and provides a referential technological means for detection of the bacillus cereus in food processing quality control, food-borne epidemic diseases and other public health events.

The invention discloses a preparation method of neutral endo-pectate lyase. The preparation method comprises the following steps: inoculating cultured mature bacillus cereus WENA703 into a seed tank from an inclined plane of an eggplant bottle, and performing secondary fermentation in a fermentation tank to produce fermented wort; and performing tubular centrifugation, plate frame filtration, ultrafiltration concentration, paperboard diatomite sterilization and standardization on the fermented wort to prepare the neutral pectate lyase with the enzyme activity being more than or equal to 300000u / ml, and performing anti-corrosion treatment to obtain standard high-activity neutral pectate lyase liquid keeping the enzyme activity to be 90% or more (at 25 DEG C for six months), wherein the bacillus cereus WENA703 is preserved with a preservation number of CCTCC NO:M2017368 in the China Center for Type Culture Collection. By adopting the technical scheme of the invention, the produced neutral pectate lyase system can better adapt to a paper-making process of waste paper.

The present invention provides DNA sequences for detecting 8 kinds of Bacillus cereus, DNA probes connected to magnetic microspheres marked with different fluorescent dyes, including: Bacillus cereus G6235, G6206, G6239, G2724, G6211, A DNA fragment selected from G6217, G6207, and G6214 respectively. Combining the Bio-Plex 200 suspension chip system of Bio-Rad, the present invention establishes a set of suspension chip detection system and its detection method for detecting 8 kinds of Bacillus cereus, which fills the gap in the detection of wax samples by molecular biology methods. The technical gap in the detection of Bacillus is of great significance for the clinical identification and epidemiological monitoring of this important pathogenic bacteria.

The invention discloses a functional gene carrier pET32a-fdhD that efficiently assists hydrogen production and a construction method thereof, and relates to the technical field of genetic engineering. The pET32a-fdhD gene vector is a pET32a plasmid containing the fdhD gene of Bacillus cereus. Its construction is to amplify the fdhD gene fragment of auxiliary formate dehydrogenase in Bacillus cereus by designing primers, and use recombinase to connect it to the pET32a fragment containing the corresponding connecting end, perform gene sequencing, and verify the integrity of the gene. The pET32a-fdhD plasmid was transformed into Escherichia coli, and sodium formate was added as a substrate in the hydrogen-producing medium, and the bacteria were cultivated to produce hydrogen. pET32a-fdhD can be applied to different bacteria, can improve hydrogen production efficiency, and can improve the existing industrial hydrogen-producing Escherichia coli.

The invention discloses bacillus cereus strain B2, a liquid preparation, a preparation method of the liquid preparation and an application in controlling rosellinia necatrix of hybridized bamboo. The strain has been preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms (CGMCC), Chaoyang District, Beijing, China, since 20th June, 2013, and has a preservation number of CGMCC No.7767 and a classified name of bacillus cereus. The above liquid preparation is diluted by 100-200 times (volume ratio) and is poured to the root of each of non-pathogeny hybridized bamboos along root system breadth direction with 100 mL per plant and once in spring every year, thereby the generation of rosellinia necatrix can be prevented. The above liquid preparation is diluted by 50-100 times (volume ratio) and is poured to the root of each of morbidity hybridized bamboos along root system breadth direction with 200 mL per plant and once in spring and once in summer every year for three successive years, thereby rosellinia necatrix can be effectively cured.