Method for obtaining leukocytes by using washing disposable leukocyte-removing plastic blood bag

A technique for removing white blood cells and white blood cells, which is applied in the field of obtaining white blood cells, and achieves the effect of simple operation, low requirements for experimental equipment, and high collection rate

Active Publication Date: 2013-10-23
济南赛尔生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no report on the patents and literatures that collect, separate and enrich the discarded leukocytes by recycling the l

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) In the 100-class clean area, use sterilized flushing fluid to initially flush the leukocyte filter device on the "disposable leukocyte-removed plastic blood bag" to remove blood stains attached to it; absorb the flushing fluid with a syringe, insert it into the blood In the tee above the bag filter device, rinse twice to remove the adhering substances;

[0022] (2) In the 100-class clean area, rinse the filter device with sterilized hypertonic solution for 30 seconds, and then immerse it in hypertonic solution for 2 minutes;

[0023] (3) In the 100-class clean area, rinse the filter device after hypertonic fluid treatment with sterilized flushing fluid, and transfer the flushing fluid containing exfoliated white blood cells to a sterile centrifuge tube;

[0024] (4) Centrifuge at 1600 rpm for 10 minutes, discard the supernatant, and collect white blood cells;

[0025] (5) In the 100-class clean area, resuspend the leukocyte pellet with sterilized leukocyte protecti...

Embodiment 2

[0032] (1) In the 100-class clean area, use sterilized flushing fluid to initially flush the leukocyte filter device on the "disposable leukocyte-removed plastic blood bag" to remove blood stains attached to it; absorb the flushing fluid with a syringe, insert it into the blood In the tee above the bag filter device, rinse 3 times to remove the adhering substances;

[0033] (2) In the 100-class clean area, rinse the filter device with sterilized hypertonic solution for 30 seconds, and then immerse it in hypertonic solution for 1 minute;

[0034] (3) In the 100-class clean area, rinse the filter device after hypertonic fluid treatment with sterilized flushing fluid, and transfer the flushing fluid containing exfoliated white blood cells to a sterile centrifuge tube;

[0035] (4) Centrifuge at 1600 rpm for 15 minutes, discard the supernatant, and collect white blood cells;

[0036] (5) In the 100-class clean area, resuspend the leukocyte pellet with sterilized leukocyte protect...

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Abstract

The invention discloses a method for obtaining leukocytes by using a washing disposable leukocyte-removing plastic blood bag. The method comprises the following steps of: removing substances adhered to a leukocyte filtering device of the disposable leukocyte-removing plastic blood bag and a tee joint above the leukocyte filtering device by washing by a sterilizing washing solution; immersing the leukocyte filtering device and the tee joint into a sterilizing hypertonic solution; washing the leukocyte filtering device treated by the sterilizing hypertonic solution by a sterilizing washing solution, and collecting the leukocytes; re-suspending a leukocyte precipitate by using a sterilizing leukocyte protecting solution, and culturing the leukocytes so that the shapes of the leukocytes are normalized; and separating, and collecting the obtained living leukocytes. The method disclosed by the invention is easy to operate, has low requirement on experimental equipment, ensures high leukocyte collection efficiency and is a simple method for recovering the precious leukocyte resources.

Description

technical field [0001] The invention relates to a method for obtaining leukocytes, in particular to a method and a special solution for obtaining leukocytes by cleaning disposable plastic blood bags without leukocytes. Background technique [0002] With the continuous use of medical methods such as clinical operations, the probability of blood transfusion has also greatly increased, but clinically only red blood cells are usually transfused in blood transfusion. At present, blood banks usually use "disposable leukocyte-removed plastic blood bags" to collect red blood cells through the leukocyte filter device in the blood bag to collect red blood cells. During this process, a large number of leukocytes remain in the leukocyte filter, blood The station disposes of it as medical waste. White blood cells are an important source of immune cells. If they are thrown away as medical waste, it will cause a lot of waste of resources. [0003] In recent years, cell therapy for cancer...

Claims

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Application Information

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IPC IPC(8): C12N5/078
Inventor 王晶波闫东伟邢晓孙帅帅王肇光田甜陈莉王惠
Owner 济南赛尔生物科技股份有限公司
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