64 results about "Leukocyte filtration" patented technology

Described herein is a method and apparatus for collecting and separating whole blood into its components, including collecting an amount of plasma and red blood cells. The collection and separation system includes a console and a disposable set. The method may include processing the blood through the centrifuge, collecting plasma, collecting red blood cells and returning red blood cells until a desired plasma volume is reached. Then a final volume of red blood cells and plasma may be collected. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle.

[Problems]To provide a membrane material that realizes effective and efficient separation of a target substance of micron size, being easy to handle and that can be worked into various forms; a blood filtration membrane and a leukocyte removing filter unit that realizes a substantial reduction of filter material volume while retaining high capability of removing leukocytes, thereby reducing the loss of hemocyte suspension; and a cell culturing diaphragm suitable for co-culturing and a relevant method of cell culturing. [Means for Solving Problems]There is provided a composite porous membrane comprising a porous membrane comprised of an organic polymeric compound, and a supporting porous membrane adjacent to the porous membrane, characterized in that the organic polymeric compound constituting the porous membrane penetrates in at least part of a surface adjacent to porous membrane of the supporting porous membrane, the porous membrane having specified opening ratio, average pore diameter, standard deviation of pore diameter, ratio of through pore, average membrane thickness, standard deviation of membrane thickness and internal structure, and that the supporting porous membrane has communicating pores of 0.5D μm or greater average pore diameter. Further, there are provided a blood filtration membrane comprising the composite porous membrane; a leukocyte removing filter unit comprising the composite porous membrane as a second filter; and, utilizing the composite porous membrane, a cell culturing diaphragm and method of cell culturing.

Described herein is a method and apparatus for collecting and separating whole blood into its components, including collecting an amount of plasma. The collection and separation system includes a console and a disposable set. The method may include processing the blood through the centrifuge, collecting the plasma, and returning red blood cells remaining in the centrifuge to the source. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle.

A leukocyte-removing filter, characterized in that it comprises a filter material having a vertical permeation coefficient (kx) of 0.5×10⁻¹² to 2.0×10⁻¹² m² and a ratio (ky/kx) of a horizontal permeation coefficient (ky) to kx of 0.5 to 1.5; and a method for removing leukocytes using said filter. The above leukocyte-removing filter has a higher leukocyte-removing performance per unit volume and also does not bring clogging nor increase of pressure drop.

Described herein is a continuous-flow centrifuge (CFC) which may be embodied in an automated system for collecting and separating whole blood into its components. The collection and separation system includes a console and a disposable set. Various blood processing procedures which produce specific blood products may be implemented by using a specific disposable set for each process. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle. The manifold and CFC disk may be included in a cassette that mounts onto the front panel of the console. The console may contain valve actuators, pressure transducers, ultrasonic sensors, a roller pump assembly, a CFC drive system, optical sensors, electronics, software, user interface components, a bar code reader and data acquisition components.

The invention discloses a combined filter membrane for efficient filtration of leukocyte, a manufacturing method thereof and a leukocyte filter. The combined filter membrane is manufactured by clamping a fiberglass layer between two polyester fiber non-woven cloth layers, and is coated with acrylic acid type copolymer to make combination of two different types of fibers solider, the hydrophilic properties of the combined filter membrane can be maintained after high-temperature steam sterilization, uniform copolymer coated on the fiberglass surface can be molten and contracted to be distributed like a pearl during high-temperature sterilization, the glass body can be exposed, the surface of the glass body contains positive charges, leukocyte contains negative charges, and the capacity of the combined filter membrane to filter leukocyte can be significantly improved due to mutual attraction of the positive charges and the negative charges. The combined filter membrane is used as a fine filter membrane to replace part of polyester non-woven cloth and is taken as a filter medium and arranged in the leukocyte filter, the comprehensive online filtration can be realized, and the rate of leukocyte filtration exceeds 99.99 percent.

Described herein is a continuous-flow centrifuge (CFC) which may be embodied in an automated system for collecting and separating whole blood into its components. The collection and separation system includes a console and a disposable set. Various blood processing procedures which produce specific blood products may be implemented by using a specific disposable set for each process. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle. The manifold and CFC disk may be included in a cassette that mounts onto the front panel of the console. The console may contain valve actuators, pressure transducers, ultrasonic sensors, a roller pump assembly, a CFC drive system, optical sensors, electronics, software, user interface components, a bar code reader and data acquisition components.

A system for filtering and processing blood that is simple to implement and that reduces the need for human intervention. The system may be used to collect red blood cells (RBCs). For such a system, a disposable set may be provided with an inlet port, an RBC container, a centrifuge rotor having a variable total volume, and a filter, along with tubing connecting the port, the container, the rotor and the filter. The filter is located in tubing between the inlet port and the rotor. A control unit is also provided and includes a spinner in which the rotor may be held, a flow-control arrangement for controlling flow among the various components of the disposable set, and an electronic controller. The whole blood is directed by the flow-control arrangement from the inlet through the filter to the rotor. The rotor includes an elastic diaphragm, and the control unit's flow-control arrangement includes a pump or other device for applying a positive and negative pressure to the rotor's elastic diaphragm. The spinner rotates the rotor so as to separate the whole blood into plasma and RBCs. Preferably, the plasma is urged out of the rotor first and returned to the donor, while the rotor is still being spun. After the plasma has been removed from the rotor, the RBCs are urged from the rotor to an RBC container. In preferred embodiments, the filter is a white blood cell filter, so that white blood cells are filtered from the blood before it reaches the rotor.

The invention relates to an isolated organ perfusion system, which comprises a liquid storage container, a centrifugal pump, a membrane lung, a first peristaltic pump, a leukocyte filter, a hemodialyzer and a waste liquid bag, wherein the liquid inlet end of the first peristaltic pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the first peristaltic pump is communicated with the liquid inlet end of the hemodialyzer through a pipeline, the liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, the waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path, and the leukocyte filter is arranged on the filtering circulation auxiliary path. According to the invention, when the leukocyte filter is blocked due to long-time work,the leukocyte filter can block the filtering circulation auxiliary path and cannot block the perfusion circulation main path, the perfusion circulation main path can still continue to work at the moment, the in-vitro organs can be continuously protected, and the leukocyte filter on the filtering circulation auxiliary path can be replaced in time.

The invention belongs to the field of leukocyte filters, and particularly relates to a leukocyte and thrombocyte filtering membrane and a preparation method thereof. The filtering membrane comprises a composite substrate and a polymer applied to the composite substrate, wherein the composite substrate is prepared from polyurethane and nano-microcrystalline cellulose according to the weight ratio of 1:(0.3-2), and the polymer is prepared from polyethylene glycol and nano-hydroxyapatite according to the weight ratio of 10:(1-2). According to the filtering membrane, leukocytes and thrombocytes can be efficiently filtered while the blood filtering speed is not decreased, the highest filtering rate of the leukocytes and the highest filtering rate of the thrombocytes can reach 99.999% and 92.4% respectively, and only 15-20 min is needed for filtering 400 ml of whole blood; meanwhile, the filtering membrane has the good flexibility, meets the requirement that moderate deformation is needed when the filtering membrane is packaged and can effectively solve the problem that the polymer is prone to be washed out in the subsequent washing process.

The invention discloses a leukocyte filter with a soft shell and a manufacturing method of the leukocyte filter. The leukocyte filter with the soft shell comprises two PVC (Polyvinyl Chloride) external membranes and a filter membrane assembly; the filter membrane assembly is arranged between the two PVC external membranes to form an unfiltered liquid cavity and a filtered liquid cavity; the filter membrane assembly comprises a combined filter membrane as well as two PVC internal membrane rings which are respectively arranged at two sides of the combined filter membranes; the internal edges of the PVC internal membrane rings are in sealed connection with the external edge of the combined filter membrane, and meanwhile, the internal membrane rings are in sealed connection with the edges of the PVC external membranes; the unfiltered liquid cavity is provided with an inlet catheter; and the filtered liquid cavity is provided with an outlet catheter and a support tube. According to the leukocyte filter with the soft shell and the manufacturing method of the leukocyte filter, the soft shell material and the filter membrane are adopted, and the binding site has the characteristics of high fusion strength, good flexibility and strong elasticity; the soft shell material and the filter membrane are capable of resisting high temperature and high pressure without breakage during the steam sterilization, and are not broken during the high-speed centrifugal separation of blood, resulting in leakage; and meanwhile, the leukocyte filter with the soft shell has the advantages of simple and attractive appearance and high utilization rate of materials and is easy to realize mass production.

The invention discloses a microfluidic chip for detecting blood related proteins of Alzheimer's disease accompanied by depression and a preparation method and application thereof. The microfluidic chip aims at the deficiencies of existing detection methods, and the microfluidic chip comprises a white blood cell filter pool, a red blood cell filter pool and a platelet filter pool. The microfluidicchip can be used to directly test a blood sample without the need of blood centrifuging to obtain serum for detection. In addition, by setting an independent microfluidic detection unit, detected related proteins do not interfere with each other. At the same time, a magnetic control valve is arranged in the chip, and the synchronization and automation of the detection can be realized by controlling a switch of an electromagnetic conversion instrument. The microfluidic chip has the advantages such as simple sample processing, short detection time, low price, controllable operation, and automatic detection.

The invention discloses a method for obtaining leukocytes by using a washing disposable leukocyte-removing plastic blood bag. The method comprises the following steps of: removing substances adhered to a leukocyte filtering device of the disposable leukocyte-removing plastic blood bag and a tee joint above the leukocyte filtering device by washing by a sterilizing washing solution; immersing the leukocyte filtering device and the tee joint into a sterilizing hypertonic solution; washing the leukocyte filtering device treated by the sterilizing hypertonic solution by a sterilizing washing solution, and collecting the leukocytes; re-suspending a leukocyte precipitate by using a sterilizing leukocyte protecting solution, and culturing the leukocytes so that the shapes of the leukocytes are normalized; and separating, and collecting the obtained living leukocytes. The method disclosed by the invention is easy to operate, has low requirement on experimental equipment, ensures high leukocyte collection efficiency and is a simple method for recovering the precious leukocyte resources.

The invention relates to a full-automatic whole blood collection and separation system, which comprises a whole blood collection and separation machine and a disposable whole blood collection and separation device, wherein the disposable whole blood collection and separation device comprises a blood collector, a sampling bag, a bacterium filter, an anticoagulant pipeline, an anticoagulant punctureoutfit, a red blood cell preservative puncture outfit, a brine puncture outfit, a suspension red blood cell bag, a leukocyte filter, a leukocyte poor suspension red blood cell bag, a pressure detector connector, a blood pump pipeline, a centrifuge cup, a plasma bag, a cryoprecipitation spare bag and a waste liquid bag; the whole blood collection and separation machine comprises an anticoagulant pump, a blood pump, a centrifugal machine, a red cell detector, a pipeline pressure detector, an anticoagulant air detector, a blood air detector, a plasma scale and a controller. According to the full-automatic whole blood collection and separation system provided by the invention, fresh plasma and suspension red cells (or washed red blood cells) are prepared through automatic whole blood collection and separation, and instead of completing collection and separation preparation in two steps, the process is operated and completed in one time, so that cost is relatively reduced and plasma quality after whole blood separation is guaranteed.

The invention belongs to the technical field of white blood cell filtering and in particular relates to a filter membrane for filtering out white blood cells and a preparation method thereof. The filter membrane for filtering out the white blood cells, provided by the invention, is mainly prepared from sodium alginate, nano zinc oxide, chitosan oligosaccharide and amino sodium lignosulphonate. The filter membrane for filtering out the white blood cells, prepared by the preparation method provided by the invention, has the advantages of strong bonding strength and high stability; in a utilization process, a condition that substances on the filter membrane are eluted or fall off can be avoided and blood pollution can be effectively prevented; meanwhile, the filter membrane for filtering out the white blood cells, provided by the invention, has a high white blood cell filtering rate and a blood platelet filtering rate; in a blood filtering process, the filter membrane has the effects of relieving or preventing a blood cold agglutination phenomenon and dredging pore-diameter blocking of the filter membrane; the filtering quality of the white blood cells can be guaranteed and the service life of the filter membrane for the white blood cells can also be prolonged, so that the filter membrane is a relatively ideal white blood cell filter membrane.

A bagged white cell filter for blood transfusion or taking blood to remove white cell from blood is composed of bag-type white cell filter with hard casing, by-pass tube, single bag or double bag or triple bag, and the blood taking single bag or double bag or triple bag. It features that when it is strongly centrifugalized, the filter can not be polluted even if its hard casing is cracked.

The invention belongs to the technical field of leukocyte filtration and relates to a method for modifying a leukocyte filtration membrane. A cheap nonwoven fabric having acid and base resistance, corrosion resistance and high chemical stability is used as a base film material and is orderly subjected to two pretreatment processes, a double-component system of acrylic acid and sodium soyate is co-grafted to the treated fabric, and the double-component system undergoes a polymerization reaction under UV irradiation to produce a polymer containing a hydrophilic carboxyl group. The method has a high grafting rate, does not produce hemolysis phenomenon and has obvious protective effects on the red blood cells. The modified filter film has high interception effects on leukocytes and platelets in the whole blood.