65 results about "Leukocyte filtration" patented technology

Described herein is a method and apparatus for collecting and separating whole blood into its components, including collecting an amount of plasma and red blood cells. The collection and separation system includes a console and a disposable set. The method may include processing the blood through the centrifuge, collecting plasma, collecting red blood cells and returning red blood cells until a desired plasma volume is reached. Then a final volume of red blood cells and plasma may be collected. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle.

[Problems]To provide a membrane material that realizes effective and efficient separation of a target substance of micron size, being easy to handle and that can be worked into various forms; a blood filtration membrane and a leukocyte removing filter unit that realizes a substantial reduction of filter material volume while retaining high capability of removing leukocytes, thereby reducing the loss of hemocyte suspension; and a cell culturing diaphragm suitable for co-culturing and a relevant method of cell culturing. [Means for Solving Problems]There is provided a composite porous membrane comprising a porous membrane comprised of an organic polymeric compound, and a supporting porous membrane adjacent to the porous membrane, characterized in that the organic polymeric compound constituting the porous membrane penetrates in at least part of a surface adjacent to porous membrane of the supporting porous membrane, the porous membrane having specified opening ratio, average pore diameter, standard deviation of pore diameter, ratio of through pore, average membrane thickness, standard deviation of membrane thickness and internal structure, and that the supporting porous membrane has communicating pores of 0.5D μm or greater average pore diameter. Further, there are provided a blood filtration membrane comprising the composite porous membrane; a leukocyte removing filter unit comprising the composite porous membrane as a second filter; and, utilizing the composite porous membrane, a cell culturing diaphragm and method of cell culturing.

Described herein is a method and apparatus for collecting and separating whole blood into its components, including collecting an amount of leukoreduced red blood cells. The collection and separation system includes a console and a disposable set. The method may include processing the blood through the centrifuge, collecting the leukoreduced red blood cells, and collecting and returning plasma to the source. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle.

Described herein is a method and apparatus for collecting and separating whole blood into its components, including collecting an amount of plasma. The collection and separation system includes a console and a disposable set. The method may include processing the blood through the centrifuge, collecting the plasma, and returning red blood cells remaining in the centrifuge to the source. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle.

An object is to provide a blood component collection system capable of collecting a desired blood component of high quality. A blood component collection system 1 comprises a centrifugal separator 20 centrifuging blood into a plurality of components, first line 21 for feeding the blood to the centrifugal separator 20, a second line 22 for releasing the blood component from the centrifugal separator 20, a temporary reservoir bag 26′ for temporarily reserving the platelet concentrate, a leukoreduction filter 261 for separating and reducing leukocyte from the platelet concentrate, and a platelet collection bag 26 for reserving the platelet concentrate having passed through the leukoreduction filter 261. The blood component collection system 1 is constructed to feed the platelet concentrate in the temporary reservoir bag 26′ to the platelet collection bag 26 through the leukoreduction filter 261, and then, feed plasma and collect the platelet remaining in the leukoreduction filter 261 (platelet concentrate from which leukocyte have been reduced) together with the plasma into the platelet collection bag 26.

A leukocyte-removing filter, characterized in that it comprises a filter material having a vertical permeation coefficient (kx) of 0.5×10−12 to 2.0×10−12 m2 and a ratio (ky / kx) of a horizontal permeation coefficient (ky) to kx of 0.5 to 1.5; and a method for removing leukocytes using said filter. The above leukocyte-removing filter has a higher leukocyte-removing performance per unit volume and also does not bring clogging nor increase of pressure drop.

Described herein is a continuous-flow centrifuge (CFC) which may be embodied in an automated system for collecting and separating whole blood into its components. The collection and separation system includes a console and a disposable set. Various blood processing procedures which produce specific blood products may be implemented by using a specific disposable set for each process. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle. The manifold and CFC disk may be included in a cassette that mounts onto the front panel of the console. The console may contain valve actuators, pressure transducers, ultrasonic sensors, a roller pump assembly, a CFC drive system, optical sensors, electronics, software, user interface components, a bar code reader and data acquisition components.

The invention discloses a combined filter membrane for efficient filtration of leukocyte, a manufacturing method thereof and a leukocyte filter. The combined filter membrane is manufactured by clamping a fiberglass layer between two polyester fiber non-woven cloth layers, and is coated with acrylic acid type copolymer to make combination of two different types of fibers solider, the hydrophilic properties of the combined filter membrane can be maintained after high-temperature steam sterilization, uniform copolymer coated on the fiberglass surface can be molten and contracted to be distributed like a pearl during high-temperature sterilization, the glass body can be exposed, the surface of the glass body contains positive charges, leukocyte contains negative charges, and the capacity of the combined filter membrane to filter leukocyte can be significantly improved due to mutual attraction of the positive charges and the negative charges. The combined filter membrane is used as a fine filter membrane to replace part of polyester non-woven cloth and is taken as a filter medium and arranged in the leukocyte filter, the comprehensive online filtration can be realized, and the rate of leukocyte filtration exceeds 99.99 percent.

Described herein is a continuous-flow centrifuge (CFC) which may be embodied in an automated system for collecting and separating whole blood into its components. The collection and separation system includes a console and a disposable set. Various blood processing procedures which produce specific blood products may be implemented by using a specific disposable set for each process. The disposable set may include a manifold, a CFC, and various components attached by tubing. These components may include one or more solution bags, blood product bags, bacterial filters, leukofilters, and a donor blood collection tube with access needle. The manifold and CFC disk may be included in a cassette that mounts onto the front panel of the console. The console may contain valve actuators, pressure transducers, ultrasonic sensors, a roller pump assembly, a CFC drive system, optical sensors, electronics, software, user interface components, a bar code reader and data acquisition components.

A system for filtering and processing blood that is simple to implement and that reduces the need for human intervention. The system may be used to collect red blood cells (RBCs). For such a system, a disposable set may be provided with an inlet port, an RBC container, a centrifuge rotor having a variable total volume, and a filter, along with tubing connecting the port, the container, the rotor and the filter. The filter is located in tubing between the inlet port and the rotor. A control unit is also provided and includes a spinner in which the rotor may be held, a flow-control arrangement for controlling flow among the various components of the disposable set, and an electronic controller. The whole blood is directed by the flow-control arrangement from the inlet through the filter to the rotor. The rotor includes an elastic diaphragm, and the control unit's flow-control arrangement includes a pump or other device for applying a positive and negative pressure to the rotor's elastic diaphragm. The spinner rotates the rotor so as to separate the whole blood into plasma and RBCs. Preferably, the plasma is urged out of the rotor first and returned to the donor, while the rotor is still being spun. After the plasma has been removed from the rotor, the RBCs are urged from the rotor to an RBC container. In preferred embodiments, the filter is a white blood cell filter, so that white blood cells are filtered from the blood before it reaches the rotor.

The invention relates to an isolated organ perfusion system, which comprises a liquid storage container, a centrifugal pump, a membrane lung, a first peristaltic pump, a leukocyte filter, a hemodialyzer and a waste liquid bag, wherein the liquid inlet end of the first peristaltic pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the first peristaltic pump is communicated with the liquid inlet end of the hemodialyzer through a pipeline, the liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, the waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path, and the leukocyte filter is arranged on the filtering circulation auxiliary path. According to the invention, when the leukocyte filter is blocked due to long-time work,the leukocyte filter can block the filtering circulation auxiliary path and cannot block the perfusion circulation main path, the perfusion circulation main path can still continue to work at the moment, the in-vitro organs can be continuously protected, and the leukocyte filter on the filtering circulation auxiliary path can be replaced in time.

An object of the present invention is to provide a method and device for filtering blood or blood components which simultaneously attains 1) efficiently increasing leukocyte removal capability and 2) effectively utilizing the filtration area by preventing nonuniform flow, when blood is filtered by being fed to a leukocyte-removing filter comprising flexible containers by being pressurized using a pump or the like. The method comprises providing a leukocyte-removing filter having a thickness of 9.0 mm to 9.4 mm, an air permeability pressure drop of 300 Pa to 700 Pa, and effective filtration area of 50 cm2 to 70 cm2 which comprises a flat and flexible container having an inlet port and an outlet port and a sheet-like leukocyte-removing-filter material with a thickness of 8.2 mm to 8.6 mm disposed to divide the container into an inlet-port side chamber and an outlet-port side chamber, providing a pair of volume restriction boards disposed outside of the inlet port side external surface and the outlet port side external surface of the flexible container, having the distance between the volume restriction boards at the portion where the volume restriction boards facing each other sandwich the leukocyte-removing filter being in a range from [thickness of leukocyte-removing filter+0.5 mm] to [thickness of leukocyte-removing filter+2.5 mm], and filtering blood or blood components through the leukocyte-removing filter under a pressure of 30 kPa to 50 kPa while restraining expansion of the flexible container occurring during filtration by means of the volume restriction boards.

The invention discloses a leukocyte filtering membrane, a manufacturing method and applications thereof. The leukocyte filtering membrane comprises a polybutylene terephthalate (PBT) non-woven fabric base material with a surface density of 50-200 g / m2 and grafting modifiers, which are grafted on the non-woven fabric base material, wherein the grafting modifier is selected from acrylic acid, methacrylic acid, vinyl pyrrolidone, and heparin or is a mixture composed of two or more of the components mentioned above; and the grafting rate of the grafting modifier is 5 to 15 wt%.

The method comprises providing a leukocyte-removing filter having a thickness of 9.0 mm to 9.4 mm, an air permeability pressure drop of 300 Pa to 700 Pa, and effective filtration area of 50 cm2 to 70 cm2 which comprises a flat and flexible container having an inlet port and an outlet port and a sheet-like leukocyte-removing-filter material with a thickness of 8.2 mm to 8.6 mm disposed to divide the container into an inlet-port side chamber and an outlet-port side chamber, providing a pair of volume restriction boards disposed outside of the inlet port side external surface and the outlet port side external surface of the flexible container, having the distance between the volume restriction boards at the portion where the volume restriction boards facing each other sandwich the leukocyte-removing filter being in a range from [thickness of leukocyte-removing filter+0.5 mm] to [thickness of leukocyte-removing filter+2.5 mm], and filtering blood or blood components through the leukocyte-removing filter under a pressure of 30 kPa to 50 kPa while restraining expansion of the flexible container occurring during filtration by means of the volume restriction boards.

Systems and methods are provided for separating a red blood cell-containing fluid into separated red blood cells and another fluid constituent. A suitable system includes a disposable fluid flow circuit and a durable, reusable separation system, with the circuit being mounted onto or otherwise associated with the separation system. The circuit includes a membrane separator for separating the fluid into its constituent parts, as well as a leukoreduction filter. The leukoreduction filter may be used before or after the red blood cell-containing fluid has been passed into the membrane separator. The red blood cell-containing fluid (if the leukoreduction filter is positioned upstream of the membrane separator) or the separated red blood cells (if the leukoreduction filter is positioned downstream of the membrane separator) may also be passed through a microaggregate filter prior to passing through the leukoreduction filter.

The invention provides a leukocyte-depleted cell filter with multiple filtering units. The filter is composed of a liquid inlet pipe (1), a flexible shell body (2), a first liquid inlet chamber (3), a first liquid outlet chamber (4), filter elements (5,13), a partition (6), a supporting pipe (7), a second liquid inlet chamber (8), a second liquid inlet chamber (9), a liquid outlet pipe (10), an inner film (11) and an auxiliary film (12). The liquid inlet pipe (1), the first liquid inlet chamber (3), the filter element (5) and the first liquid outlet chamber (4) compose the first filtering unit. The second liquid inlet chamber (8), the filtering element (13), the second liquid outlet chamber (9) and the liquid outlet pipe (10) compose the second filtering units. Totally, the two filtering units which are independent to each other are formed. Blood passes through the two filtering units, the leukocyte-depleting effect is greatly improved, and meanwhile the phenomena that the area of the single filtering unit is prevented from being excessively large, the utilization efficiency of filtering materials is not high and lateral leakage happens to the edge of the filter are avoided. By means of the leukocyte-depleted cell filter, non-leakage can be kept for two minutes under the 50Kpa compressed air. Under the temperature ranging from 115 DEG C to 121 DEG C, high-pressure steam sterilizing is carried out for half an hour. After sterilization, adhesion and deformation do not happen to the inside and the outside of the device and the large application value is achieved.

The invention belongs to the field of leukocyte filters, and particularly relates to a leukocyte and thrombocyte filtering membrane and a preparation method thereof. The filtering membrane comprises a composite substrate and a polymer applied to the composite substrate, wherein the composite substrate is prepared from polyurethane and nano-microcrystalline cellulose according to the weight ratio of 1:(0.3-2), and the polymer is prepared from polyethylene glycol and nano-hydroxyapatite according to the weight ratio of 10:(1-2). According to the filtering membrane, leukocytes and thrombocytes can be efficiently filtered while the blood filtering speed is not decreased, the highest filtering rate of the leukocytes and the highest filtering rate of the thrombocytes can reach 99.999% and 92.4% respectively, and only 15-20 min is needed for filtering 400 ml of whole blood; meanwhile, the filtering membrane has the good flexibility, meets the requirement that moderate deformation is needed when the filtering membrane is packaged and can effectively solve the problem that the polymer is prone to be washed out in the subsequent washing process.

The invention discloses a leukocyte filter with a soft shell and a manufacturing method of the leukocyte filter. The leukocyte filter with the soft shell comprises two PVC (Polyvinyl Chloride) external membranes and a filter membrane assembly; the filter membrane assembly is arranged between the two PVC external membranes to form an unfiltered liquid cavity and a filtered liquid cavity; the filter membrane assembly comprises a combined filter membrane as well as two PVC internal membrane rings which are respectively arranged at two sides of the combined filter membranes; the internal edges of the PVC internal membrane rings are in sealed connection with the external edge of the combined filter membrane, and meanwhile, the internal membrane rings are in sealed connection with the edges of the PVC external membranes; the unfiltered liquid cavity is provided with an inlet catheter; and the filtered liquid cavity is provided with an outlet catheter and a support tube. According to the leukocyte filter with the soft shell and the manufacturing method of the leukocyte filter, the soft shell material and the filter membrane are adopted, and the binding site has the characteristics of high fusion strength, good flexibility and strong elasticity; the soft shell material and the filter membrane are capable of resisting high temperature and high pressure without breakage during the steam sterilization, and are not broken during the high-speed centrifugal separation of blood, resulting in leakage; and meanwhile, the leukocyte filter with the soft shell has the advantages of simple and attractive appearance and high utilization rate of materials and is easy to realize mass production.

Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.

The invention discloses a microfluidic chip for detecting blood related proteins of Alzheimer's disease accompanied by depression and a preparation method and application thereof. The microfluidic chip aims at the deficiencies of existing detection methods, and the microfluidic chip comprises a white blood cell filter pool, a red blood cell filter pool and a platelet filter pool. The microfluidicchip can be used to directly test a blood sample without the need of blood centrifuging to obtain serum for detection. In addition, by setting an independent microfluidic detection unit, detected related proteins do not interfere with each other. At the same time, a magnetic control valve is arranged in the chip, and the synchronization and automation of the detection can be realized by controlling a switch of an electromagnetic conversion instrument. The microfluidic chip has the advantages such as simple sample processing, short detection time, low price, controllable operation, and automatic detection.

The invention relates to a device and a method for separating hematopoietic stem cells from cord blood. The device for separating hematopoietic stem cells from cord blood comprises a blood sampling bag, a plasma bag, an erythrocyte collecting bag, an eluant bag, a stem cell collecting bag, a leukocyte filter and a pressurizing pump, wherein a coagulum filtering net is arranged between the blood sampling bag and the plasma bag; another coagulum filtering net and the leukocyte filter are arranged between the blood sampling bag and the eluant bag; the pressurizing pump is arranged between the plasma bag and the erythrocyte collecting bag and is used for achieving reverse filling of plasma. The device is simple, the use method is simple and convenient, the recycling rate of the prepared hematopoietic stem cells is greater than 83%, the hematopoietic stem cells are high in activity, less time is spent, the recycling efficiency is high, a new method is provided for hematopoietic stem cell preparation, and relatively good practical application values and popularization values are achieved.

The invention relates to a full-automatic whole blood collection and separation system, which comprises a whole blood collection and separation machine and a disposable whole blood collection and separation device, wherein the disposable whole blood collection and separation device comprises a blood collector, a sampling bag, a bacterium filter, an anticoagulant pipeline, an anticoagulant punctureoutfit, a red blood cell preservative puncture outfit, a brine puncture outfit, a suspension red blood cell bag, a leukocyte filter, a leukocyte poor suspension red blood cell bag, a pressure detector connector, a blood pump pipeline, a centrifuge cup, a plasma bag, a cryoprecipitation spare bag and a waste liquid bag; the whole blood collection and separation machine comprises an anticoagulant pump, a blood pump, a centrifugal machine, a red cell detector, a pipeline pressure detector, an anticoagulant air detector, a blood air detector, a plasma scale and a controller. According to the full-automatic whole blood collection and separation system provided by the invention, fresh plasma and suspension red cells (or washed red blood cells) are prepared through automatic whole blood collection and separation, and instead of completing collection and separation preparation in two steps, the process is operated and completed in one time, so that cost is relatively reduced and plasma quality after whole blood separation is guaranteed.

The invention belongs to the technical field of white blood cell filtering and in particular relates to a filter membrane for filtering out white blood cells and a preparation method thereof. The filter membrane for filtering out the white blood cells, provided by the invention, is mainly prepared from sodium alginate, nano zinc oxide, chitosan oligosaccharide and amino sodium lignosulphonate. The filter membrane for filtering out the white blood cells, prepared by the preparation method provided by the invention, has the advantages of strong bonding strength and high stability; in a utilization process, a condition that substances on the filter membrane are eluted or fall off can be avoided and blood pollution can be effectively prevented; meanwhile, the filter membrane for filtering out the white blood cells, provided by the invention, has a high white blood cell filtering rate and a blood platelet filtering rate; in a blood filtering process, the filter membrane has the effects of relieving or preventing a blood cold agglutination phenomenon and dredging pore-diameter blocking of the filter membrane; the filtering quality of the white blood cells can be guaranteed and the service life of the filter membrane for the white blood cells can also be prolonged, so that the filter membrane is a relatively ideal white blood cell filter membrane.

A bagged white cell filter for blood transfusion or taking blood to remove white cell from blood is composed of bag-type white cell filter with hard casing, by-pass tube, single bag or double bag or triple bag, and the blood taking single bag or double bag or triple bag. It features that when it is strongly centrifugalized, the filter can not be polluted even if its hard casing is cracked.

There is disclosed a leukocyte removal filter material comprising a nonwoven fabric having polybutylene terephthalate fiber. The average fiber diameter of the nonwoven fabric is 0.9 to 1.5 μm, the formation index corresponding to a thickness of 0.3 mm of the nonwoven fabric is 15 to 70, and when the average fiber diameter of the nonwoven fabric is X and the specific surface area of the nonwoven fabric is Y, X and Y satisfy the following relational expression (1):Y≧−0.65×X+1.75   (1).

The invention belongs to the technical field of leukocyte filtration and relates to a method for modifying a leukocyte filtration membrane. A cheap nonwoven fabric having acid and base resistance, corrosion resistance and high chemical stability is used as a base film material and is orderly subjected to two pretreatment processes, a double-component system of acrylic acid and sodium soyate is co-grafted to the treated fabric, and the double-component system undergoes a polymerization reaction under UV irradiation to produce a polymer containing a hydrophilic carboxyl group. The method has a high grafting rate, does not produce hemolysis phenomenon and has obvious protective effects on the red blood cells. The modified filter film has high interception effects on leukocytes and platelets in the whole blood.