Filter membrane for filtering out white blood cells and preparation method thereof

A white blood cell and filter membrane technology, applied in the field of white blood cell filtration, can solve the problems of filter membrane clogging, blood pollution, and low service life, and achieve the effect of improving filtration and filtration rate

Active Publication Date: 2017-09-29
广州达济医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the current white blood cell filter membrane only focuses on the design of cell rate filtration. During use, the filter membrane is often clogged, resulting in a short service life or defects that affect the quality of cell

Method used

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  • Filter membrane for filtering out white blood cells and preparation method thereof
  • Filter membrane for filtering out white blood cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, a kind of preparation of nano zinc oxide

[0042] a. Take zinc nitrate and add it to deionized water, stir until completely dissolving, then add sodium carbonate, the weight ratio of described zinc nitrate and sodium carbonate is 1.4:1, stir until the reaction is complete, centrifuge, get white precipitate;

[0043] b. add dodecyl glucoside to the white precipitate obtained in step a, stir for 6min, the solid-liquid ratio of the white precipitate and dodecyl glucoside is 1g:12ml, then add trimethylolpropane three Methacrylate, stirred for 15min, the solid-to-liquid ratio of the white precipitate and trimethylolpropane trimethacrylate was 1g:8ml to obtain a mixed solution;

[0044] c. Heat the mixture obtained in step b to 135°C, keep it warm for 35 minutes, then pass in nitrogen, continue heating to 280°C, keep it warm for 50 minutes;

[0045] d. Cool the mixed solution obtained in step c to 40° C., add absolute ethanol, and centrifuge to obtain a precipi...

Embodiment 2

[0046] Embodiment 2, the preparation of a kind of amino lignosulfonate sodium

[0047] A. Sodium lignosulfonate is dissolved in water to prepare an aqueous solution of sodium lignosulfonate with a concentration of 70% by mass percentage, heated to 70°C, and triethylenetetramine is added. The added amount of triethylenetetramine is lignosulfonate 25% of the total volume of sodium bicarbonate aqueous solution, then dripping the glutaraldehyde aqueous solution that the mass percent concentration is 50%, the rate of addition of the glutaraldehyde aqueous solution is 0.4mL / min, and the time of adding is controlled at 25min, at a temperature of React at 85°C for 2 hours to obtain sodium aminolignosulfonate solution;

[0048] B. Extract the sodium amino lignosulfonate solution obtained in step A with absolute ethanol to obtain the extract, wash the extract with petroleum ether, pre-freeze at -8°C for 3 hours after washing, and then freeze it under vacuum It is 0.05mPa, the temperatu...

Embodiment 3

[0049] Embodiment 3, a kind of filter membrane for filtering leukocyte

[0050] The filter membrane used to filter out white blood cells consists of the following raw materials and parts by weight:

[0051] 100 parts of sodium alginate, 35 parts of nano zinc oxide, 15 parts of chitosan oligosaccharides and 10 parts of amino lignosulfonate sodium; the nano zinc oxide is the nano zinc oxide prepared in Example 1, and the amino lignosulfonate Sodium acid sodium is the amino lignosulfonate sodium prepared in embodiment 2.

[0052] Preparation:

[0053] S1 Add sodium alginate to water, stir until completely dissolved to obtain an aqueous solution of sodium alginate;

[0054] S2 Add chitosan oligosaccharide and amino lignosulfonate sodium into water, stir until completely dissolved to obtain a mixed solution;

[0055] S3 adding nano-zinc oxide into water, stirring until completely dissolved, to obtain a nano-zinc oxide solution;

[0056] S4 mixes the mixed solution obtained in s...

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Abstract

The invention belongs to the technical field of white blood cell filtering and in particular relates to a filter membrane for filtering out white blood cells and a preparation method thereof. The filter membrane for filtering out the white blood cells, provided by the invention, is mainly prepared from sodium alginate, nano zinc oxide, chitosan oligosaccharide and amino sodium lignosulphonate. The filter membrane for filtering out the white blood cells, prepared by the preparation method provided by the invention, has the advantages of strong bonding strength and high stability; in a utilization process, a condition that substances on the filter membrane are eluted or fall off can be avoided and blood pollution can be effectively prevented; meanwhile, the filter membrane for filtering out the white blood cells, provided by the invention, has a high white blood cell filtering rate and a blood platelet filtering rate; in a blood filtering process, the filter membrane has the effects of relieving or preventing a blood cold agglutination phenomenon and dredging pore-diameter blocking of the filter membrane; the filtering quality of the white blood cells can be guaranteed and the service life of the filter membrane for the white blood cells can also be prolonged, so that the filter membrane is a relatively ideal white blood cell filter membrane.

Description

technical field [0001] The invention belongs to the technical field of leukocyte filtration, and in particular relates to a filter membrane for filtering leukocytes and a preparation method thereof. Background technique [0002] In the medical process of blood transfusion, a variety of side effects of blood transfusion often occur. The root cause is that the transfusion of leukocyte-containing whole blood or blood components can cause non-hemolytic febrile reaction, human leukocyte antigen alloimmunization, transfusion-associated viral infection and post-transfusion graft Adverse transfusion reactions such as anti-host disease. Therefore, it is of great significance to filter out leukocytes in blood preparations before blood transfusion. Leukocyte filter is an important method to filter out leukocytes. Its principle is to use the principle of mechanical block and adsorption to intercept leukocytes, and let red blood cells with smooth surface and strong deformability pass th...

Claims

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Application Information

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IPC IPC(8): B01D71/74B01D67/00C01G9/02B82Y30/00B82Y40/00A61M1/34C08H7/00
CPCA61M1/3496B01D67/0079B01D71/74B82Y30/00B82Y40/00C01G9/02C01P2004/64C08H6/00
Inventor 王衡东
Owner 广州达济医学科技有限公司
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