Fibroblast liquid for beauty treatment and preparation method thereof

A fibroblast and cell technology, which is used in cosmetic preparations, skin care preparations, pharmaceutical formulations, etc., can solve the problems that cannot effectively promote epidermal cell metabolism, life quality and physical health threats, and does not have biologically active substances, etc. problems, to achieve the effect of low production technology requirements, high practical value, and prevention of stains

Inactive Publication Date: 2013-11-20
艾普瑞斯(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing fine chemical skin care products contain substances that are harmful to the skin such as lead, mercury, alcohol, preservatives, hormones, spices, etc. When using beauty care products, they also cause greater damage to the skin, and even The resulting stains, red blood streaks, allergies and other problems pose a great threat to people's quality of life and health
[0003] Most of the existing beauty care products are made by chemical methods, and there are many harmful substances in the products, and long-term use will have adverse effects on the skin
Moreover, most of the existing products do not have biologically active substances, which cannot effectively promote the metabolism of epidermal cells and improve the luster of the skin.
Moreover, some substances contained in chemical skin care products will cause allergic reactions to the human body, so they are not suitable for all people.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The technical scheme adopted by the present invention is: a fibroblast liquid for beauty maintenance and a preparation method thereof, the fibroblast liquid contains cell culture liquid, collagen, cell growth factors, amino acids and vitamins; the method for preparing the fibroblast liquid The method comprises the steps of obtaining umbilical cord tissue dermal cells, primary culture of umbilical cord tissue dermal cells, subculture of umbilical cord tissue dermal cells, collecting cell culture fluid, and adding glycerin and cytokines to prepare fibroblast fluid.

Embodiment 2

[0031] On the basis of Example 1, in order to realize the production and preparation of fibroblast fluid in the present invention, a preferred embodiment also includes the steps of preparing the fibroblast fluid for cosmetic maintenance as follows:

[0032] ① Obtain umbilical cord tissue cells: wash the umbilical cord tissue with phosphate buffer solution containing penicillin and streptomycin for 3 times, cut the umbilical cord tissue into small pieces, spread them on a plate with the dermis facing down, add dispase II to the plate and culture overnight ; After overnight, separate the epidermis and dermis, cut the dermis into small pieces and inoculate them in a culture dish containing fibroblast culture medium to obtain umbilical cord tissue cells;

[0033] ②Primary culture of umbilical cord tissue cells: the obtained umbilical cord tissue dermal cells were primary cultured, and the fibroblast culture medium was replaced every three days; More fibroblasts to prepare cell cul...

Embodiment 3

[0039] On the basis of Example 2, in order to efficiently obtain umbilical cord tissue dermal cells in the present invention, a preferred embodiment also includes cutting the dermis into 1mm in the first step 3 In order to facilitate the rapid growth of dermal cells and avoid internal cell death; the condition of overnight culture in the first step is overnight culture at 4°C; in order to ensure the rapid and massive growth of primary cultured dermal cells in the operation, the primary The conditions for culturing umbilical cord tissue dermal cells were as follows: 0.5 ml of fibroblasts were added to the culture dish and cultured at a constant temperature of 37°C, the volume fraction of carbon dioxide gas in the incubator was 5%, and the other parts were exactly the same as in Example 2.

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PUM

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Abstract

The invention discloses a fibroblast liquid for beauty treatment and a preparation method thereof. The fibroblast liquid contains a cell culture solution, collagens, cell growth factors, amino acids and vitamins, wherein the cell growth factors comprise fibroblast growth factors, epidermal cell growth factors, hepatocyte growth factors and keratinocyte growth factors. The preparation method of the fibroblast liquid comprises the following steps: acquiring umbilical cord tissue hypodermal cells, carrying out primary culture of the umbilical cord tissue hypodermal cells, subcultring the umbilical cord tissue hypodermal cells, collecting the obtained cell culture liquid, and adding glycerin and cytokines to prepare the fibroblast liquid. The fibroblast liquid has the efficacies of epidermal cell metabolism promotion, skin elasticity enhancement, injured epidermal cell restoration, color spot removal and the like; and the preparation method is simple, is easy to operate, and is suitable for the large-scale growth preparation of stem cell liquids.

Description

technical field [0001] The invention relates to a biological cosmetic and a preparation method thereof, in particular to a fibroblast liquid for beauty maintenance and a preparation method thereof. Background technique [0002] With the improvement of people's living standards, people pay more and more attention to beauty care, and there are many kinds of beauty care products on the market. However, most of the existing fine chemical skin care products contain substances that are harmful to the skin such as lead, mercury, alcohol, preservatives, hormones, spices, etc. When using beauty care products, they also cause greater damage to the skin, and even The problems such as spots, red blood streaks, and allergies produced have caused a great threat to people's quality of life and health. [0003] Most of the existing beauty care products are made by chemical methods, and there are many harmful substances in the products, and long-term use will have adverse effects on the ski...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/64A61K8/65A61Q19/00
Inventor 丁彬彬
Owner 艾普瑞斯(北京)生物科技有限公司
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