Grifola frondosa health care oral solution and preparation method thereof

Abstract

The invention discloses a grifola frondosa health care oral solution and a preparation method thereof. The preparation method comprises the following steps: taking a grifola frondosa fungus as an original strain and obtaining a grifola frondosa liquid strain; inoculating the grifola frondosa liquid strain to a solid culture-medium, prepared by wild herbage including miscanthus floridulus, jujun grass, pennisetum purpureum, dicranopteris linearis and the like according to specific proportion, , performing solid fermentation for 25-40 days, taking out a fermentation hyphostroma product, obtaining an extracting solution rich in grifola frondosa polysaccharide through the fermentation hyphostroma product in a hot water leaching method, and making the extracting solution into a concentrate; mixing the concentrate and Chinese medicine liquid prepared by decocting traditional blood sugar reduction Chinese medicines according to a mass ratio of 1:(0.5-1) to prepare the oral solution with a good taste. According to the invention, the content of the grifola frondosa polysaccharide obtained via the solid fermentation hyphostroma can reach 3.05 percent; the product is subjected to an animal blood sugar reduction test, the test shows that the grifola frondosa health care oral solution has an effect of reducing I-type high blood sugar model mouse blood sugar caused by Alloxan, and the blood sugar reduction ratio can reach 41.10 percent; in addition, the preparation method is stable and applicable to industrial production.

Description

technical field

[0001] The invention belongs to the fields of health food and biomedicine, and in particular relates to a frondosa frondosa health oral liquid and a preparation method thereof. Background technique

[0002] Grifola frondosa belongs to Basidiso mycotina, Hgmenomycetes, HoLobasidiomy ceidae, AphyLLophraLes, PoLrrIordeaL , the fungus of the genus Origola, also known as polyporus bay leaf, dry Buddha mushroom, chestnut mushroom, lotus mushroom, etc., is called Maitake in Japan. As early as 1984, Japanese scholar Ohno et al. reported for the first time that Grifola frondosa polysaccharides have anti-tumor activity, and the National Cancer Institute of the United States confirmed in 1992 that the extract of Grifola frondosa has the effect of resisting HIV; subsequently, a large number of scientific researches It has been shown that only Grifola frondosa polysaccharides have the effects of anti-tumor, improving immunity, lowering blood sugar, anti-virus, anti-radia...

Examples

Embodiment 1

[0030] A preparation method of Grifola frondosa health-care oral liquid comprises the following steps:

[0031] (1) Preparation of Grifola frondosa solid fermentation strain:

[0032] A. Activate and rejuvenate the preserved species of Grifola frondosa with PDA medium, put them in a 25°C incubator and cultivate them statically for 10 days to obtain the production slant species, and put them in the refrigerator for later use; use the shake flask culture medium to expand the production slant species , to obtain the first-class species for producing shake flasks;

[0033] PDA medium formula: peeled potato 200 g / L, glucose 20 g / L, agar 20 g / L, add water to the required volume, and the pH is natural;

[0034] Shake flask medium formula: glucose 30g / L, peptone 5 g / L, KH 2 PO 4 1 g / L, MgSO 4 ·7H 2 O 1g / L, VB 1 0.05g / L, pH natural; culture conditions: with 100mL / 500mL volume, insert 1cm 2 The production slant species were cultivated on a rotary shaker at 80 rpm for 7 days at ...

Embodiment 2

[0048] A preparation method of Grifola frondosa health-care oral liquid comprises the following steps:

[0049] (1) Preparation of Grifola frondosa solid fermentation strain:

[0050] A. Activate and rejuvenate the preserved species of Grifola frondosa with PDA medium, put them in a 32°C incubator and cultivate them statically for 15 days to obtain the production slant species, put them in the refrigerator for later use; use the shake flask culture medium to expand the production slant species , to obtain the first-class species for producing shake flasks;

[0051] PDA medium formula: peeled potato 200 g / L, glucose 20 g / L, agar 20 g / L, add water to the required volume, and the pH is natural;

[0052] Shake flask medium formula: glucose 60 g / L, peptone 15 g / L, KH 2 PO 4 3 g / L, MgSO 4 ·7H 2 O 2 g / L, VB 1 0.15 g / L, natural pH; culture conditions: 200mL / 500mL, insert 1cm 2 The production slant species were cultivated on a rotary shaker at 200rpm for 10 days at a temperatu...

Embodiment 3

[0066] A preparation method of Grifola frondosa health-care oral liquid comprises the following steps:

[0067] (1) Preparation of Grifola frondosa solid fermentation strain:

[0068] A, the Grifola frondosa preserved species is activated and rejuvenated with PDA medium, and placed in a 28°C incubator for static cultivation for 12 days to obtain the production slant species, which is stored in the refrigerator for subsequent use; the production slant species is expanded and cultivated with a shake flask medium , to obtain the first-class species for producing shake flasks;

[0069] PDA medium formula: peeled potato 200 g / L, glucose 20 g / L, agar 20 g / L, add water to the required volume, and the pH is natural;

[0070] Shake flask medium formula: glucose 45 g / L, peptone 10 g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O1.5 g / L, VB 1 0.10 g / L, pH natural; culture conditions: with 150mL / 500mL volume, insert 1cm 2 The production slant species were cultured on a rotary shaker at 120rpm...

technical field

[0001] The invention belongs to the fields of health food and biomedicine, and in particular relates to a frondosa frondosa health oral liquid and a preparation method thereof. Background technique

[0002] Grifola frondosa belongs to Basidiso mycotina, Hgmenomycetes, HoLobasidiomy ceidae, AphyLLophraLes, PoLrrIordeaL , the fungus of the genus Origola, also known as polyporus bay leaf, dry Buddha mushroom, chestnut mushroom, lotus mushroom, etc., is called Maitake in Japan. As early as 1984, Japanese scholar Ohno et al. reported for the first time that Grifola frondosa polysaccharides have anti-tumor activity, and the National Cancer Institute of the United States confirmed in 1992 that the extract of Grifola frondosa has the effect of resisting HIV; subsequently, a large number of scientific researches It has been shown that only Grifola frondosa polysaccharides have the effects of anti-tumor, improving immunity, lowering blood sugar, anti-virus, anti-radia...

Examples

Embodiment 1

[0030] A preparation method of Grifola frondosa health-care oral liquid comprises the following steps:

[0031] (1) Preparation of Grifola frondosa solid fermentation strain:

[0032] A. Activate and rejuvenate the preserved species of Grifola frondosa with PDA medium, put them in a 25°C incubator and cultivate them statically for 10 days to obtain the production slant species, and put them in the refrigerator for later use; use the shake flask culture medium to expand the production slant species , to obtain the first-class species for producing shake flasks;

[0033] PDA medium formula: peeled potato 200 g / L, glucose 20 g / L, agar 20 g / L, add water to the required volume, and the pH is natural;

[0034] Shake flask medium formula: glucose 30g / L, peptone 5 g / L, KH 2 PO 4 1 g / L, MgSO 4 ·7H 2 O 1g / L, VB 1 0.05g / L, pH natural; culture conditions: with 100mL / 500mL volume, insert 1cm 2 The production slant species were cultivated on a rotary shaker at 80 rpm for 7 days at ...

Embodiment 2

[0048] A preparation method of Grifola frondosa health-care oral liquid comprises the following steps:

[0049] (1) Preparation of Grifola frondosa solid fermentation strain:

[0050] A. Activate and rejuvenate the preserved species of Grifola frondosa with PDA medium, put them in a 32°C incubator and cultivate them statically for 15 days to obtain the production slant species, put them in the refrigerator for later use; use the shake flask culture medium to expand the production slant species , to obtain the first-class species for producing shake flasks;

[0051] PDA medium formula: peeled potato 200 g / L, glucose 20 g / L, agar 20 g / L, add water to the required volume, and the pH is natural;

[0052] Shake flask medium formula: glucose 60 g / L, peptone 15 g / L, KH 2 PO 4 3 g / L, MgSO 4 ·7H 2 O 2 g / L, VB 1 0.15 g / L, natural pH; culture conditions: 200mL / 500mL, insert 1cm 2 The production slant species were cultivated on a rotary shaker at 200rpm for 10 days at a temperatu...

Embodiment 3

[0066] A preparation method of Grifola frondosa health-care oral liquid comprises the following steps:

[0067] (1) Preparation of Grifola frondosa solid fermentation strain:

[0068] A, the Grifola frondosa preserved species is activated and rejuvenated with PDA medium, and placed in a 28°C incubator for static cultivation for 12 days to obtain the production slant species, which is stored in the refrigerator for subsequent use; the production slant species is expanded and cultivated with a shake flask medium , to obtain the first-class species for producing shake flasks;

[0069] PDA medium formula: peeled potato 200 g / L, glucose 20 g / L, agar 20 g / L, add water to the required volume, and the pH is natural;

[0070] Shake flask medium formula: glucose 45 g / L, peptone 10 g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O1.5 g / L, VB 1 0.10 g / L, pH natural; culture conditions: with 150mL / 500mL volume, insert 1cm 2 The production slant species were cultured on a rotary shaker at 120rpm...

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