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Method for extracting high-purity aesculetin from euphorbia lathyris fruit

A kind of qintedrin, high-purity technology, applied in the direction of organic chemistry, etc., can solve the problems of low purity, high cost, low yield, etc., and achieve the effect of simple extraction process, easy operation and low cost

Inactive Publication Date: 2013-11-27
LESHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People such as Yu Ling of Liaoning Medical College used polyamide column chromatography to separate and prepare tervetrin B with a purity of 50% from the extract of Cortex chinensis; Jing Wang of Liaoning Medical College et al. Extract from the separation and preparation of fencetine with a purity of 98%; some people also use macroporous resin to separate and purify fencetine from chicory extract, but its purity is very low
These research reports all have shortcomings such as low purity or complicated process, high cost, and low yield.
[0005] At present, there is no research report on the extraction of high-purity aferetin from the fruit of Scaperia spp. and it is suitable for industrial production

Method used

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  • Method for extracting high-purity aesculetin from euphorbia lathyris fruit
  • Method for extracting high-purity aesculetin from euphorbia lathyris fruit
  • Method for extracting high-purity aesculetin from euphorbia lathyris fruit

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Take 2000g of caperia sativa fruit, crush it, squeeze out the oil, and obtain cake residue, use 10L of ethyl acetate to extract the cake residue at 60°C for 0.5h under reflux, filter, and then use ethyl acetate to filter the residue 10L was refluxed and extracted at 60°C for 0.5h. The two filtrates were combined. Concentrate the filtrate under reduced pressure to obtain an extract, dissolve the extract in 200mL of chloroform, stir well, filter, and discard the filtrate; repeat twice to obtain a precipitate; slowly wash the obtained precipitate with 200mL of acetone to obtain a light yellow Powdered solid, dried to obtain 2.5g of fencetin with a purity greater than 95%.

[0022] Adopt high-performance liquid chromatography, adopt conventional peak area normalization method to determine the purity of this compound, the result shows: the peak area of ​​fencetin accounts for more than 95% of total peak area, represents that the purity of cerebroide is greater th...

Embodiment 2

[0023] Example 2: Take 2000g of caperia sativa fruit, crush it, squeeze out the oil, and obtain cake residue, reflux extract 10L of cake residue with acetone at 60°C for 0.5h, filter, and then use 10L of acetone for the filter residue, and extract at 60°C Extract under reflux for 0.5h. The two filtrates were combined. Concentrate the filtrate under reduced pressure to obtain an extract, dissolve the extract in 200mL of dichloromethane, stir well, filter, and discard the filtrate; repeat 2 times to obtain a precipitate; slowly wash the precipitate with 200mL of acetone to obtain a light yellow Powdered solid, dried to obtain 2.4g of fencetine with a purity greater than 95%.

[0024] Adopt high-performance liquid chromatography, adopt conventional peak area normalization method to determine the purity of this compound, the result shows: the peak area of ​​fencetin accounts for more than 95% of total peak area, represents that the purity of cerebroide is greater than 95%; 2000g...

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Abstract

The invention relates to a method for extracting high-purity aesculetin from euphorbia lathyris fruit. The method for extracting high-purity aesculetin from the euphorbia lathyris fruit comprises the following steps of: squeezing oil from the obtained euphorbia lathyris fruit so as to obtain cake residues, and soaking or refluxing the cake residues for 0.5-72 hours twice to three times by using an organic solvent at 10-60 DEG C, thus obtaining an organic solvent leaching liquor of the euphorbia lathyris fruit; concentrating the organic solvent leaching liquor to obtain an extract; dissolving the extract by using a right amount of low-polarity organic solvent, filtering to obtain precipitates, repeatedly dissolving the precipitates by using the low-polarity organic solvent twice to three times, and filtering to obtain the precipitates; washing the precipitates obtained in the end by using a small amount of acetone twice to three times, filtering to obtain pale yellow precipitates, and drying the prepared faint yellow precipitates to obtain the aesculetin with the purity higher than 95%. The method has the advantages of rapidness, high yield, low cost and the like. The purity of the prepared aesculetin is higher than 95%, and the yield of the aesculetin can reach up to 0.12%.

Description

technical field [0001] The invention relates to the field of separation of natural products, in particular to a method for extracting high-purity aferetin from caperides fruit. Background technique [0002] Esculetin (esculetin), also known as esculetin, 6,7-dihydroxycoumarin, is the main active ingredient of Chinese herbal medicines such as esculetin, caper fruit (Dianjinzi), and viola. Percetrin is the intermediate of the drug Pingchuanning, and Pingchuanning is methylated with dimethyl sulfate to obtain Pingchuanning (dimethoxycoumarin), which is an antiasthmatic drug with an antiasthmatic effect Obvious, and have expectorant, antitussive effect. Peridotidin itself is an antibacterial drug, which has inhibitory effect on Shigella, can be used to treat acute bacillary dysentery, and has anti-asthma and expectorant effects, and can also be used to treat chronic bronchitis. [0003] Many traditional Chinese medicine prescriptions or Chinese patent medicines such as Tuihuan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/16
Inventor 熊俊如陈封政李书华
Owner LESHAN NORMAL UNIV
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