32 results about "Caper spurge" patented technology

The invention discloses traditional Chinese medicine mask powder for whitening and dispelling freckle, which has the advantages of little toxicity and capability of effectively achieving whitening, dispelling freckle and the like. A formulation for the mask powder comprises 3 to 10 grams of ginseng, 3 to 15 grams of rhizome polygonati, 6 to 15 grams of radix asparagi, 3 to 10 grams of rhizome typhonii, 3 to 10 grams of angelica dahurica, 6 to 15 grams of angelica, 3 to 10 grams of white stiff silkworm, 6 to 15 grams of fructus xanthil, 3 to 10 grams of bletilla, 6 to 15 grams of white gourd seed, 3 to 15 grams of gingko leaf, 3 to 10 grams of fragrant solomonseal rhizome, 3 to 10 grams of puncturevine, 3 to 10 grams of euphorbia lathyris, 1 to 6 grams of pearl powder, 1 to 6 grams of litharge, 3 to 10 grams of mint, 1 to 3 grams of human placenta, 3 to 6 grams of cinnamon, 3 to 10 grams of tasselblower, 3 to 10 grams of leucophanite, 1 to 3 grams of borneol, 10 to 30 grams of green bean powder, and 3 to 10 grams of honey-fried licorice root.

The present invention relates to an extraction and separation method of caper spurge seed sterol. Said method successively includes the following steps: (1), extraction: using low-polarity solvent to make refluxing extraction; (2), column chromatography: respectively collecting fraction containing caper spurge seed sterol and collecting fraction containing no caper spurge seed sterol, making the fraction containing caper spurge seed sterol undergo the processes of concentration and crystallization, concentrating the fraction containing no caper spurge seed sterol and recovering solvent, and discarding concentrated paste; (3), crystallization: eluting the fraction containing caper spurge seed sterol, making distillation and concentration at normal pressure, and crystallizing for 24-3hr at room temperature, filtering to obtain crystal crude product; (4), recrystallization: heating and dissolving said crystal crude product in low-polarity mixed solvent, filtering to obtain crystal product.

The invention relates to a plaster for treating mastitis, hyperplasia of mammary glands, breast lumps, join thydrops, traumatic injury, furunculosis and malignant sore. The plaster is prepared by major ingredients and minor ingredients; wherein the major ingredient comprises raw materials such as herba lycopi, euphorbia lathyris, scirpus maritimus, rhizoma zedoariae and the like; the minor ingredients comprise the following raw materials such as Zhangdan and balsam. The invention overcomes the shortcoming of the prior art and has efficacies of relaxing the muscles, stimulating the blood circulation, diminishing inflammation, killing pain, dewetting and removing ringworm, has better effects on treating mastitis, hyperplasia of mammary glands, breast lumps, join thydrops, traumatic injury, furunculosis and malignant sore, is characterized by simple preparation process, fast response, good effects, non poison and side effect and provides preparation method and application thereof.

The present invention provides ingenol and a preparation method therefor. The method comprises: by taking euphorbia lathyris seeds as raw materials, obtaining the ingenol through recovery, extraction, hydrolysis reaction and silica gel column chromatography. According to the ingenol and the preparation method therefor, types off solvents used in the whole preparation process are less; both an extraction solvent and an extracting solvent are methanol; the solvent can be reused, so that the production cost is reduced, and the production process is simplified; the hydrolysis reaction of an ingenol esterified compound is performed by use of a strong base; by virtue of this reaction, an esterified compound of the ingenol can be converted into the ingenol, so that the separation process is simplified; and the ingenol with purity higher than 98% can be acquired through silica gel column chromatography for separation, so that use of a high-pressure reversed phase chromatography system is avoided, and the separation conditions are simplified. By use of the preparation method provided by the present invention, industrial preparation of the ingenol can be achieved, the preparation yield is high, and the product purity is high.

The invention belongs to the technical field of the biological medicine, and relates to a microbial conversion method for a euphorbia lathyris diterpene compound. The method comprises the following steps: performing the microbial conversion to euphorbia lathyris phorbol and 7-hydroxy euphorbia lathyris phorbol by using Nocard's bacillus (i) Nocardia sp. (/i) NRRL 5646, and preparing 3-carbonyl euphorbia lathyris phorbol and 7-acetoxyl euphorbia lathyris phorbol derivatives. Proved by the experiment, the polarity of the 3-carbonyl euphorbia lathyris phorbol in the derivative is reduced. Compared with a substrate, the derivative has the better cytotoxic activity. The derivative is an antitumor lead compound with development value.

The invention relates to the field of biological medicines, and relates to a conversion method of a euphorbia lathyris diterpene compound. The method comprises the following steps of utilizing a known microorganism to perform microorganism conversion on the euphorbia lathyris phorbol and a derivative thereof, and combining with chemical acylation, so as to prepare a euphorbia lathyris diterpene derivative compound; especially, utilizing a mucor circinelloides (CICC40242) microorganism to perform hydroxylation biological conversion on the euphorbia lathyris phorbol and euphorbia factor L3, and performing chemical acylation on hydroxyls introduced by C8 and C18, so as to obtain three hydroxylated products and six C8 alcohol esterified products. The method has the advantages that the water solubility of the euphorbia factor L3 is improved by the hydroxylation at site, and the druggability is improved; furthermore, the prepared euphorbia lathyris diterpene compound can be used for preparing anti-tumor activity and anti-tumor multidrug resistance type pharmaceutical compositions. The chemical formula is shown in the specification.

The invention provides a weeding compound used for killing and preventing gramineous weeds in paddy fields. The weeding compound is ethyl(2R)-2-[4-(4-cyano 2-fluorophenoxy)-phenoxy]propionic ester. A preparation method comprises following steps: hydroquinone and (S)-(-)-2-chloropropionic acid are subjected to Williamson etherification reaction at alkaline conditions so as to obtain (R)2-[4-hydroxy-phenoxy]propionic acid; reaction with a strong base is carried out so as to obtain (R)2-[4-(4-cyano 2-fluorophenoxy)-phenoxy]propionic acid; and reaction with a strong base is carried out so as to obtain ethyl(2R)-2-[4-(4-cyano 2-fluorophenoxy)-phenoxy]propionic ester. The preparation method is simple; reaction is mild; no high temperature high pressure condition is needed; reaction time is relatively short; yield is high; the weeding compound can be used for killing Euphorbia lathyris with high efficiency, and can also be used for killing crab grass, paspalum distichum, green bristlegrass, eleusine indica, and alopecurus aequalis, and possesses excellent killing effect on barnyard grass with drug resistance; and the using amount is less than that of cyhalofop-butyl.

The invention relates to moleplant seed composite essential oil extracted from three types of plant seeds, comprising the following raw materials based on parts by weight: 50-70 parts of moleplant seed (Euphorbia lathyris), 20-30 parts of pepperweed seed and 10-45 parts of the seed of Chinese dodder. In the invention, the three types of plant seeds such as the moleplant seed (Euphorbia lathyris), the pepperweed seed and the seed of Chinese dodder are cleaned, dried, and crushed into fine powder, then are proportionally mixed, and then extracted with a CO2 supercritical fluid extraction technology to obtain the moleplant seed composite essential oil. The moleplant seed composite essential oil has the efficiencies of treating podagra, cervical spondylosis, scapulohumeral periarthritis, arthritis, Parkinson syndrome and the like, and also has the obvious effects of skin tendering, skin whitening, beverage removing, breast enhancing, ovary nursing and the like.

The invention discloses a preparation method of lathyrol. The preparation method includes: a, taking semen euphorbia lathyris (which is bought in the market) as a raw material, and pulverizing semen euphorbia lathyris to obtain semen euphorbia lathyris powder; b, performing reflux extraction with a proper solvent, combining extracts, and performing reduced-pressure concentration to obtain stiff paste; c, adding alkali liquor to the stiff paste for hydrolysis; d, adding acid to the alkali-hydrolyzed liquid to adjust the pH value to 6-7; e, performing reduced-pressure concentration to the neutralized hydrolysate till stiff paste is obtained, and performing silica gel column chromatography to obtain coarse lathyrol; and f, re-crystallizing the obtained coarse lathyrol with a mixed solvent ofpetroleum ether and ethyl acetate to obtain a crystal product. The preparation method is simple to operate, less in solvent consumption, and stable in process, and is suitable for laboratorial and industrial preparation.

The invention provides a high-efficient feed for reducing attack rate of live pigs, and relates to the technical field of cultivation of live pigs. The high-efficient feed is prepared from 150-180 parts of corn, 135-150 parts of wheat bran, 100-120 parts of rice bran, 15-25 parts of table salt, 10-12 parts of garden balsam, 8-14 parts of pyrola herbs, 2-6 parts of winged euonymus twigs, 7-16 parts of trumpetcreeper flowers, 4-9 parts of commiphora myrrha, 1-6 parts of rhizoma ligustici wallichii, 4-7 parts of star anise, 5-10 parts of pericarpium trichosanthis, 2-8 parts of lindera glauca, 4-10 parts of Longyansuo, 7-15 parts of myrobalan, 5-12 parts of panicled fameflower roots, 3-8 parts of millettia specisoa roots, 3-8 parts of hydrocotyle sibthorpioides, 7-14 parts of dried toxicodendron vernicifluum resins, 14-18 parts of radix phytolacca acinosa, 10-15 parts of euphorbia lathyris, 7-12 parts of hemp seeds, 3-7 parts of folium sennae and 2-8 parts of java brucea fruits. The high-efficient feed disclosed by the invention has the beneficial effects of being capable of complementing daily nutrients for the live pigs, having the functions of strengthening resistance and enhancing immunity, and being capable of reducing the generation probability that the live pigs are ill and dead.

The invention belongs to the technical field of natural medicinal chemistry and particularly relates to a quick identification method for ingenol. The method successively includes the steps of: 1) performing alkali hydrolysis, neutralization and extraction to an upper layer obtained after solvent recovery to an alcohol extract liquid of Euphorbia lathyris, volatilizing the solvent out, and adding methanol for dissolution; 2) collecting a supernatant and performing thin-layer chromatographic experiment, wherein the sample is developed with petroleum ether (60-90 DEG C) - ethyl acetate as a developing agent; 3) taking a thin-layer plate out, air-drying the thin-layer plate, spraying a color developing agent onto the thin-layer plate for color development, and heating the thin-layer plate until stripes can be clearly seen; 4) observing the thin-layer plate with wavelength being 365 nm, wherein in a spectral map, light-blue color spots represent for the ingenol, while orange, cyan and pink spots represent for other substances. The method is high-effective, simple, accurate and reliable, fills the gap of specific quick detection and identification for the ingenol, and can be used for quick detection of the ingenol.

The invention relates to a patch for treating breast cancer to be ulcerated, and belongs to the technical field of traditional Chinese medicine preparations, wherein the patch mainly comprises six bulk drugs such as angelica, bolbostemma paniculatum, euphorbia lathyris, realgar and the like, and the bulk drugs are prepared into a paste for external application. The patch of the present invention has functions of qi and blood smoothing, lump softening, knot loosening, middle warmer strengthening and evil eliminating, and further has characteristics of good treatment effect on breast cancer to be ulcerated, easy sticking, safety, and effectiveness.

The invention provides an euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol. The euphorbia lathyris callus cell suspension culture method capable ofextracting and obtaining the ingenol comprises the following steps: 1) inoculating a callus induction medium with roots of aseptic seedlings of euphorbia lathyris as an explant, and carrying out culturing so as to obtain callus; 2) cutting the callus into small pieces, inoculating a subculture medium with the cut callus, and carrying out subculture; 3) shredding the callus after the subculture, inoculating a liquid medium used for carrying out suspension culture with the shredded callus, and carrying out oscillation culture; 4) renewing culture solution used for carrying out the suspension culture, and carrying out subculture; and 5) collecting suspension cells as a raw material, and carrying out separation and preparation so as to obtain the ingenol and lathyrol. The euphorbia lathyris suspension cells obtained by the euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol are fast in proliferation rate; and moreover, the ingenol and the lathyrol can be stably extracted from the euphorbia lathyris suspension cells, thereby providing technical support for large-scale euphorbia athyris cell culture and industrial production of ingenol biosynthesis. Thus, the euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol has important application values.

The invention discloses a pomegranate flower paste for promoting blood circulation to arrest pain. The pomegranate flower paste is prepared from pomegranate flowers, olibanum, cottonrose hibiscus leaves, rhizome corydalis, corydalis amabilis, dichotomanthes tristaniicarpa, rumex dentatus, gerbera, artemisia anomalas, euphorbia lathyris, alumen, lactosucrose, salt, cane sugar, lactobacillus and edible acetic acid. According to the pomegranate flower paste for promoting blood circulation to arrest pain, the fresh pomegranate flowers are soaked with the salt solution, sterilization can be achieved, and the flower color is kept; after vacuum freeze drying is performed, the color is gorgeous, the nutrient content is high, and storage is easy; various traditional Chinese medicines are added, the medicines and the pomegranate flowers supplement each other, blood circulation is promoted, bleeding is arrested, toxin is eliminated, detumescence is achieved, inflammation is diminished, and pain is relieved; the edible acetic acid is added, the toxic and side effect of the traditional Chinese medicines is avoided, the pomegranate flowers are fermented through lactic acid, micromolecule prebiotics substances are generated, the taste is tartish, the sweetness is proper, and digestive absorption is easy; oligosaccharide and salt are added, hyperlipidemia, hypertension and hyperglycemia can be prevented, healthful intestinal microflora multiplication is promoted, decayed teeth are prevented, and the pomegranate flower paste is easy and convenient to eat, and suitable for the modern fast-tempo life style.

The invention discloses an euphorbia lathyris large ring diterpenoid compound and an application of the euphorbia lathyris large ring diterpenoid compound in preparation of drugs used for treating liver cancer, lung cancer and stomach cancer. The euphorbia lathyris large ring diterpenoid compound is prepared by extracting euphorbia lathyris fruits, and a chromatographic process identifies that the disclosed by the invention is a novel euphorbia lathyris large ring diterpenoid compound. Experiments show that the euphorbia lathyris large ring diterpenoid compound has the effect of strongly inhibiting liver cancer (SMMC-7221), lung adenocarcinoma (L342) and gastric adenocarcinoma (MCc80-3).

The invention provides a novel compound shown in a formula (I), and a preparation method of the compound shown in the formula (I). Proved by experiment results, the novel compound has the advantages that the fully novel compound is separated from seeds of euphorbia lathyris; the compound has excellent inhibiting function on cancer cells, and can be used for preparing anti-tumor medicines. The preparation method of the compound has the advantages that the reaction conditions are moderate, the energy consumption is low, the efficiency is high, the cost is low, the green and environment-friendlyeffects are realized, and the like; the preparation method is very suitable for industrialized application. (The formula (I) is shown in the attached figure.).

The invention relates to a whitening Chinese medicinal composition, which is characterized by comprising the following Chinese medicinal materials in part by mass: 1 to 3 parts of circassian bean, 4 to 6 parts of angelica dahurica, 4 to 6 parts of euphorbia lathyris leaf, 4 to 6 parts of cherry leaf and 1 to 3 parts of jasmine flowers. The whitening Chinese medicinal composition is prepared by a method comprising: grinding the five Chinese medicinal materials, uniformly mixing the five Chinese medicinal materials, adding water in an amount which is 20 times the total mass of the five Chinese medicinal materials, soaking at 60 to 70 DEG C for 5 hours, separating medicinal liquid, concentrating, spray-drying and grinding.

The invention relates to a traditional Chinese medicine for treating bone injury for animals, and is characterized by comprising the following components by weight ratio: 50g of talcum, 2g of Mingxiong, 1.5g of musk, and 5g of Euphorbia lathyris, and if difficult urination is generated, 15g of bamboo leaves, 15g of wick, 15g of amber, and 20g of cinnabar re added. An application method is characterized in that talcum is immersed with water, the liquid is taken, and slag is removed, after the frying of capparis spinosa, the above raw material components are ground to fine powder, and the yellowwine is used for processing. The traditional Chinese medicine has the advantages of small side effects and good therapeutic effects.

The invention discloses a captive local pig feed. The captive local pig feed is prepared from, by weight, 68-75 parts of corn, 51-55 parts of wheat, 38-45 parts of sorghum, 40-50 parts of rice bran, 20-25 parts of sweet potatoes, 30-40 parts of grape peel pomace, 40-55 parts of distillers' grains, 6-8 parts of conyza bonariensis, 6-8 parts of roughhaired holly roots, 4-6 parts of herb of euphorbia lathyris L. and 4-6 parts of herba equiseti arvensis. Pigs fed on the feed are high in pure port rate, little in fat, good in port quality and high in slaughtering speed, the slaughtering time is about 2 months ahead of that of local pigs, and then the pork yield is greatly increased. Pork is bright red in color, mellow and full, clear in marble texture and good in fat precipitation ratio, lean pork is tender, smooth and not blunt, fatty pork is mellow and not greasy, the taste is delicious, and the mouthfeel is pure.