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Extraction and separation method of moleplant seed sterol

A technique for the separation of stephania sterols, which is applied in the field of extracting effective parts of plants, can solve the problems of complex process, difficult operation, and low yield, and achieve the effects of simple process, good purity, and high yield

Inactive Publication Date: 2007-01-31
XIAN HAO TIAN BIO ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a method for extracting and isolating sterols, so as to overcome the problems of complex process, difficult operation and low yield in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Extraction: Get 500kg of the pulverized Stephania chinensis raw material, put it into the seepage column, then use 50°C petroleum ether, extract by seepage, get the extract 3000kg extract, extract under reduced pressure distillation and concentrate to 400kg (containing solute 30%). Place it at room temperature, and detect through high-pressure liquid phase (HPLC): the extract contains 2.16 kg of sterol, and the calculated extraction rate is 92%.

[0022] (2) Column chromatography: the concentrated paste is loaded onto a 150kg silica gel chromatography column for adsorption and sample loading. After loading, the impurities are eluted with eluent sherwood oil: ethyl acetate=95:5, when the eluent When the echinosterol is contained in the ethanol, use petroleum ether:ethyl acetate=90:10 to elute the echinosterol, and start to collect the effluent, and combine the collection solution containing the echinosterol.

[0023] The developer system is petroleum ether: ethyl ac...

Embodiment 2

[0031] (1) Extraction: Get 500kg of the pulverized Stephania chinensis raw material, put it into the seepage column, then use 50°C n-hexane, seepage extraction, get the extract 3500kg extract, extract under reduced pressure distillation and concentrate to 420kg (containing solute 33%). Place it at room temperature, and detect through high-pressure liquid phase (HPLC): the extract contains 2.18 kg of sterol, and the calculated extraction rate is 95%.

[0032] (2) Column chromatography: the concentrated paste is put on a 150kg silica gel chromatography column for adsorption and sample loading. After loading, the impurities are eluted with eluent n-hexane:ethyl acetate=95:5. When the eluent When the echinosterol is contained in the ethanol, use n-hexane:ethyl acetate=90:10 to elute the echinosterol, and start to collect the effluent, and combine the collected liquid containing the echinosterol.

[0033] The developer system is petroleum ether: ethyl acetate = 4:1; the thin-layer...

Embodiment 3

[0041] (1) Extraction: Get 500kg of the pulverized Stephania chinensis raw material, put it into the seepage column, then use 50°C n-hexane, seepage extraction, get the extract 3300kg extract, the extract is distilled under reduced pressure and concentrated to 400kg (containing solute 35%). Place it at room temperature, and detect through high pressure liquid phase (HPLC): the extract contains 2.17 kg of sterol, and the calculated extraction rate is 94%.

[0042] (2) Column chromatography: the concentrated cream is put on a 150kg silica gel chromatography column for adsorption and sample loading. After loading, the impurities are eluted with eluent n-hexane: acetone=96: 4. When the eluent contains In the case of stepherol, use n-hexane: ethyl acetate = 92:8 to elute stepherol, and begin to collect the effluent, and combine the collected solution containing stepherol.

[0043] Detected by high-pressure liquid phase (HPLC): 2.1 kg of stepherol is contained in the stepherol elua...

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Abstract

The present invention relates to an extraction and separation method of caper spurge seed sterol. Said method successively includes the following steps: (1), extraction: using low-polarity solvent to make refluxing extraction; (2), column chromatography: respectively collecting fraction containing caper spurge seed sterol and collecting fraction containing no caper spurge seed sterol, making the fraction containing caper spurge seed sterol undergo the processes of concentration and crystallization, concentrating the fraction containing no caper spurge seed sterol and recovering solvent, and discarding concentrated paste; (3), crystallization: eluting the fraction containing caper spurge seed sterol, making distillation and concentration at normal pressure, and crystallizing for 24-3hr at room temperature, filtering to obtain crystal crude product; (4), recrystallization: heating and dissolving said crystal crude product in low-polarity mixed solvent, filtering to obtain crystal product.

Description

Technical field: [0001] The invention relates to the technical field of extracting effective parts of plants, in particular to a method for extracting and separating sterols. Background technique: [0002] Qianjinzi, English name Caper Euphorbia Seed, Latin name Semen Euphorbiae, is the dry mature seed of Euphorbia lathyris L., a dicotyledonous plant medicine Euphorbia. Euphobiasteroid (Euphobiasteroid), that is, epoxy capitol phenylacetic acid diacetate (5,10-Diacetyl-6,20-epoxy-3-phenylacetyl-latyrol), has the following effects: 1. Laxative effect: strength About 3 times that of castor oil. 2. Anti-tumor effect: It has inhibitory effect on acute lymphocytic type, granulocytic type, chronic granulocytic type and acute monocytic leukemia. 3. Inhibiting the formation of spots: mainly used in skin care products, treating sunspots, removing warts, whitening, etc. The current extraction method is: after the raw material is pulverized, it is refluxed with water-containing lowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/17A61K36/47A61K131/00
Inventor 胡洪来
Owner XIAN HAO TIAN BIO ENG TECH
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