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Preparation method and application of tyrosyl tRNA (transfer ribonucleic acid) synthetase mutant

A synthase and tyrosyl technology, which is applied in the biological field, can solve the problems such as reports on the application of a tyrosyl tRNA synthetase mutant preparation method and the like, and achieve the effect of a simple preparation method

Inactive Publication Date: 2013-11-27
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] But so far, there are no reports about the preparation method of tyrosyl tRNA synthetase mutants and their application in adjuvant therapy after radiotherapy and chemotherapy

Method used

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  • Preparation method and application of tyrosyl tRNA (transfer ribonucleic acid) synthetase mutant
  • Preparation method and application of tyrosyl tRNA (transfer ribonucleic acid) synthetase mutant
  • Preparation method and application of tyrosyl tRNA (transfer ribonucleic acid) synthetase mutant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Materials and methods

[0080] Major equipment

[0081] (1) Ultra-clean workbench (Boxun Industrial Co., Ltd.)

[0082] (2) 5L Bioflow3000 fermenter (NBS, USA)

[0083] (3) C25KC Shaker (NBS, USA)

[0084] (4) UV spectrophotometer (BioRad)

[0085] (5) Freeze dryer (US FTS)

[0086] (6) AKTA explore purification system (GE Amersham)

[0087] (7) High-pressure homogenizer (Panda Plus 2000, Niro Soavi, Italy)

[0088] (8) Microplate reader (perkin Elmer 1420, VICTOR3).

[0089] Material

[0090] (1) Strains and plasmids: Tmax high-expressing strain BL21 (F-ompT hsdS (rB-mB-) gal dcm, Novagen) and anti-Tmax monoclonal antibody were provided by aTyr Pharma in the United States;

[0091] (2) Purification filler: SP-Sepharose Fast Flow; Sephadax-G50; Q-Sepharose Fast Flow (Pharmaei);

[0092] (3) Protein building, yeast extract (Oxoid), casein hydrolyzate (Sigma);

[0093] (4) The rest are domestic analytically pure.

[0094] Media and common solutions

[...

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Abstract

The invention belongs to the technical field of biology, relates to a preparation method and an application of a tyrosyl tRNA (transfer ribonucleic acid) synthetase mutant, and in particular to a preparation method of a tyrosyl tRNA synthetase mutant and an application of the tyrosyl tRNA synthetase mutant in assistant treatment after radiotherapy and chemotherapy. The preparation method is simple and quick, and is applicable to industrial production; pharmacodynamics studying results indicate that the tyrosyl tRNA synthetase mutant can be used for treating thrombocytopenia induced by tumor radiotherapy and chemotherapy.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method and application of a tyrosyl tRNA synthetase mutant; in particular, it relates to a preparation method of a tyrosyl tRNA synthetase mutant and its application in adjuvant therapy after radiotherapy and chemotherapy . Background technique [0002] It is known that aminoacyl tRNA synthetase is the earliest protein in the evolution of life, and it is widely found in various organisms such as animals, plants, bacteria, and viruses; this type of enzyme catalyzes the transfer of amino acids to the corresponding tRNA, Activate amino acids, and then participate in protein synthesis, ensuring the rigor and diversity of living organisms. The formation of specific aminoacyl tRNA depends on the correct recognition of amino acid and tRNA by aminoacyl tRNA synthetase, and the specificity of protein biosynthesis mainly depends on the correct pairing of the anticodon of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C07K1/18A61K38/53A61P7/04
Inventor 莫炜于敏詹领郎永江张艳玲包志宏
Owner FUDAN UNIV
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