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Application of oligomannuronic acid in the preparation of drugs, health products or dietary supplements for preventing and treating alcoholic liver injury

A technology for polymannuronic acid and alcohol-induced liver injury, applied in the field of marine medicine, can solve the problems that have not been seen before, achieve broad development and application prospects, inhibit oxidative stress, and improve the effect of antioxidant capacity and GSH level.

Active Publication Date: 2016-04-20
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are domestic and foreign literature reports that oligomannuronic acid has various biological activities such as anti-oxidation, anti-inflammation, immune regulation and anti-senile dementia, but there is no research on its application in alcoholic liver injury and hangover. to report

Method used

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  • Application of oligomannuronic acid in the preparation of drugs, health products or dietary supplements for preventing and treating alcoholic liver injury
  • Application of oligomannuronic acid in the preparation of drugs, health products or dietary supplements for preventing and treating alcoholic liver injury
  • Application of oligomannuronic acid in the preparation of drugs, health products or dietary supplements for preventing and treating alcoholic liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Preparation of different molecular weight oligomeric mannuronic acids

[0022] Prepare polymannuronic acid (Mw=10.4kD, ie M-10K) into suspensions of different concentrations with pure water, and sonicate for 1min to fully dissolve it. Put it into 8 microwave digestion tanks (2mL per tank), set the power to 800W, microwave degradation under different temperature conditions for different times (see Table 1 for reaction conditions), the obtained product is cooled, filtered with double-layer filter paper, and freeze-dried , to obtain the mannuronic acid oligosaccharide mixture, freeze-dry to obtain oligomeric mannuronic acid with different molecular weights, namely M-2K, M-3K, M-4K, M-5K.

[0023] The structural formula of the prepared oligomannuronic acid is as follows: in the formula, R=H or Na.

[0024]

[0025] Using TOSOHTSKGEL3000PWXL column chromatography (7.8mm*300mm, 6μm) and dextran standard (China Institute for the Control of Pharmaceutical and ...

Embodiment 2

[0029] Example 2: Inhibitory effect of oligomannuronic acid on alcohol-induced increase in mouse liver body mass index

[0030] 84 Kunming mice of 18-22g were randomly divided into control group, model group, and administration group (different weight-average molecular weight oligomannuronic acid, Mw=2kD, Mw=3kD, Mw=4kD, Mw=5kD , Mw=10kD), 12 in each group. Adaptive feeding was carried out for 5 days. During the experiment period, the mice were free to eat common feed and water, 200 mg / kg was given once, and 4.8 g / kg alcohol was given once 2 hours later, and water was not allowed. After 16 hours, the mice were sacrificed, the liver was separated, rinsed twice with pre-cooled PBS (0.1M, pH=7) solution, and then the water on the surface of the organ was blotted dry with filter paper, weighed, and the organ index was calculated. The calculation formula is as follows: organ index (%)=organ weight (g) / body weight (g)*100%, and the results are shown in Table 2.

[0031] Table 2 In...

Embodiment 3

[0035] Example 3: Inhibitory effect of oligomannuronic acid on the increase of TG and TC levels in alcohol-induced mouse serum

[0036] 84 Kunming mice of 18-22g were randomly divided into control group, model group, and administration group (different weight-average molecular weight oligomannuronic acid, Mw=2kD, Mw=3kD, Mw=4kD, Mw=5kD, Mw=10kD), 12 in each group. Adaptive feeding for 5 days. During the experiment period, the mice were free to eat common feed and drinking water. 200mg / kg was given once, 4.8g / kg alcohol was given once 2h later, water was not allowed to eat, and blood was taken from the eyeball after 16h, prepared at 3000rpm×10min Serum, determination of TG, TC indicators. as shown in Table 3.

[0037] Table 3 The inhibitory effect of different molecular weight oligomeric mannuronic acids on the increase of TC and TG induced by alcohol

[0038]

[0039] ##P<0.01, compared with normal control group; *P<0.05, **P<0.01, compared with model group; (n=12; X±SD)...

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Abstract

The invention provides application of mannuronic acid oligomers in preparing medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Mannuronic acid oligomers with different molecular weights, which are obtained by degrading polymannuronic acid graded and purified from algin, can lower the hepatosomatic index increase caused by alcoholic injury, resist triglyceride and cholesterol increase caused by alcohol, effectively lower glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase increase in serum caused by alcohol, enhance the total oxidation resistance and glutathione level of the serum, lower the mouse drunkenness rate, prolong the mouse drunkenness time, and shorten the sober-up time, thereby implementing the protective action and hangover alleviating function on alcoholic hepatic injury. The products provided by the invention are derived from marine natural products, have the advantages of abundant resources, high industrialization tendency, high safety, high effectiveness and the like, and have wide development and application prospects in the aspects of prevention and treatment of alcoholic hepatic injury, hangover alleviation and liver protection.

Description

technical field [0001] The invention belongs to the field of marine drugs, and in particular relates to the application of oligomannuronic acid in the preparation of drugs, health care products or dietary supplements for preventing and treating alcoholic liver injury. Background technique [0002] Liver damage caused by excessive alcohol consumption is a worldwide medical problem. Alcoholic fatty liver (ALD) is an early manifestation of alcohol-induced liver damage, which can develop into alcoholic hepatitis, liver fibrosis, liver cirrhosis and even liver cancer. In developed countries such as Europe and the United States, alcoholic liver disease and its complications are one of the important causes of death. In recent years, the incidence of ALD in my country has also been increasing year by year, the degree of liver damage is aggravating, and the number of damaged functional organs is increasing, which has caused systemic harm to the health of the Chinese population. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K31/702A61K31/7016A61P1/16A23L29/30A23L33/125
Inventor 郝杰杰管华诗李春霞胡婷李全才于广利
Owner OCEAN UNIV OF CHINA
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