Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity

A technology of in vitro germination and pollen germination, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, plant cells, etc., can solve the problems of less research on in vitro culture of lettuce pollen, achieve stable and reliable measurement results, and cultivate Good effect, easy to observe the effect

Inactive Publication Date: 2013-12-18
WUHAN VEGETABLE RES INST
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the pollen of various plants has been successfully cultured in vitro, such as lily, tobacco and other in vitro culture techniques are quite mature, but there are few studies on the in vitro culture of lettuce pollen in Compositae

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity
  • Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity
  • Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of lettuce pollen in vitro germination liquid culture medium, with distilled water as solvent, the component that it comprises is ME 3 , 170 or 180 or 190g·L -1 PEG4000 and 90 or 100 or 110g·L -1 sucrose, adjust the pH value of the medium to 5.8 or 5.9 or 6.0.

[0036] The ME 3 The components are: potassium nitrate (KNO 3 ) 950 or 1000 or 1050mg·L -1 , ammonium chloride (NH 4 Cl) 261.25 or 275 or 288.75 mg·L -1 , calcium nitrate tetrahydrate (CaNO 3 4H 2 O) 1520 or 1600 or 1680, potassium dihydrogen phosphate (KH 2 PO 4 ) 80.75 or 85 or 89.25 mg·L -1 , potassium chloride (KCl) 166.25 or 175 or 183.75 mg·L -1 , Magnesium sulfate heptahydrate (MgSO 4 ·7H 2 O) 475 or 500 or 525 mg·L -1 , Potassium iodide (KI) 0.83mg·L -1 , boric acid (H 3 BO 3 ) 100mg·L -1 , copper sulfate pentahydrate (CuSO 4 ·5H 2 O) 0.025mg·L -1 , Sodium molybdate dihydrate (Na 2 MoO 4 2H 2 O) 0.25mg·L -1 , cobalt chloride hexahydrate (CoCl 2 ·6H 2 O) 0.025mg·L -1 , di...

Embodiment 2

[0042] A method for measuring the pollen vigor of lettuce pollen germination in vitro by liquid medium, the steps are as follows:

[0043] 1. Collection of lettuce pollen:

[0044] The lettuce pollen was collected from the lettuce population of the Wuhan Institute of Vegetable Science Breeding Base in early June 2013. The collection method was as follows: select lettuce plants with normal development, full flower buds, no pests and diseases, and strong growth. :30 Select the inflorescence that is blooming, pick the inflorescence together with the flowering branches, quickly move it into a fresh-keeping bag equipped with clear water, and bring it back to the laboratory for standby. 2. Screening of lettuce pollen germination conditions in vitro: 2.1 Screening of PEG concentration for lettuce pollen germination in vitro:

[0045] PEG4000 is a high molecular weight compound that mainly plays an osmotic adjustment role. Spread the fresh pollen evenly and add 0g·L respectively -1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lactuca sativa L. pollen in vitro germination culture medium and a method for measuring pollen activity. The lactuca sativa L. pollen in vitro germination culture medium uses distilled water as a solvent and comprises components of ME3 (Monnior), PEG4000 (Polyethylene Glycol) and saccharose. The method comprises the following steps: (1), collecting pollen, namely, selecting an inflorescence flowering in that day, transferring into a freshness protection package filled with clear water, and bringing back in a room; (2), preparing a pollen in vitro liquid culture medium; (3), spreading and culturing pollen, namely, horizontally placing a glass slide loaded with the liquid culture medium on an experiment platform, removing petals of a capitulum by using tweezers, stripping off a synandrium stamen and then taking an anther out, enabling the anther to slide on the surface of the culture medium to ensure that pollen is uniformly spread and distributed on the surface of the culture medium in a single layer and a pollen tube grows; (4), measuring the pollen activity, namely, after in vitro culturing, observing, and counting a pollen germination rate, wherein the pollen germination rate reflects a proportion of vital pollens. The method is rapid, simple and easy to operate, good in culturing effect, and large in practical application value; abundant vital germinating pollens are obtained, and the pollen activity is effectively measured.

Description

technical field [0001] The invention belongs to the technical field of pollen viability measurement of vegetable plants, in particular to a lettuce pollen germination liquid medium in vitro, and also relates to a method for measuring pollen vigor by using the liquid medium to culture lettuce pollen germination in vitro. Background technique [0002] Lettuce (Lactuca sativa L.) belongs to Compositae (Compositae), Lactuca (Lactuca) one or two-year herbaceous plant, native to the Mediterranean coast, likes cold climate, not high temperature tolerance. The content of carbohydrates in lettuce is low, rich in inorganic salts and vitamins, especially niacin, which has diuretic and blood pressure-lowering effects on patients with high blood pressure and heart disease. Therefore, lettuce is widely cultivated all over the world and has become the main vegetable that people like. [0003] In recent years, as consumers' demand for lettuce has increased, the demand for high-quality and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12Q1/02
Inventor 张润花周国林王斌才汪爱华黄兴学姚芳胡侦华邓耀华林处发
Owner WUHAN VEGETABLE RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com