Bactrian camel specific antibody preparation method and immune detection method

A technology for antibodies and labeled antibodies, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve the problems that the best conditions have not been reported

Active Publication Date: 2015-03-11
吉日木图 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the optimal conditions for the immunization process of camel antibodies

Method used

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  • Bactrian camel specific antibody preparation method and immune detection method
  • Bactrian camel specific antibody preparation method and immune detection method
  • Bactrian camel specific antibody preparation method and immune detection method

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Experimental program
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Embodiment Construction

[0028] 1. Preparation of polyclonal antibodies

[0029] The experimental animals were divided into two groups: blank group (3 peaks) and experimental group (3 peaks). Before the initial immunization, 20 mL of negative blood was drawn from the neck vein, the serum was separated, and the aseptic aliquots were frozen at -40°C. The blank group was immunized with normal saline each time; the experimental group was immunized with 0.1mg / ml BSA for 4 times. Blood was collected from the neck vein of the camel before each immunization and 15 days after the final immunization, and the serum was separated.

[0030] 2. Preliminary establishment of ELISA detection method

[0031] 2.1 Preparation of rabbit anti-camel IgG enzyme-labeled secondary antibody

[0032] 1) Purification of camel IgG by affinity chromatography;

[0033] ① Wash and fix Protein A on the iron frame. After packing the packing into the column, wash off the protective agent, namely ethanol, with 10 column volumes of ultrapure wa...

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Abstract

The invention relates to an optimal immune procedure and method of an immune camel. The immune method comprises the step of multipoint subcutaneous injecting at the back of a Bactrian camel, wherein the immune period is two weeks, the injected antigen dosage is 1mg / ml, and the immune is carried out three times. The invention further relates to a preparation flow and a method of a rabbit anti-camel IgG enzyme-labeled secondary antibody, a method for detecting camel anti-serum titer by applying the rabbit anti-camel IgG enzyme-labeled secondary antibody through ELISA and an optimal detection condition.

Description

(1) Technical field [0001] The present invention belongs to the technical field of antibody engineering. The present invention takes Sunit Bactrian camel in Inner Mongolia as the research object, and uses bovine serum albumin (BSA) to prepare anti-BSA camel polyclonal antibodies to optimize immune conditions. The rabbit anti-camel IgG enzyme-labeled secondary antibody was prepared, and an indirect ELISA detection kit was established. (2) Background technology [0002] The immune system is divided into innate immunity and adaptive immunity, of which adaptive immunity is divided into humoral immunity and cellular immunity. [0003] The reason why antiserum can react with related antigens is because the antiserum contains related antibody proteins. In the early 1960s, animal proteins with antibody activity were defined as immunoglobulins. Mainly found in plasma, but also found in other body fluids, tissues and some secretions. Generally, the antigenicity of the L chain on the Ig mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535
Inventor 吉日木图刘微玉斯日古楞
Owner 吉日木图
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