Semen freezing method for bactrian camels

Abstract

The invention discloses a seminal fluid freezing method of a bactrian camel, which comprises the following steps: diluting collected seminal fluid and liquefied fluid according to a ratio of 1: 1, and storing in a 37 DEG C constant-temperature box to fully liquefy; carrying out microscopic examination on the fully liquefied semen, and evaluating the sperm motility; performing centrifugal treatment on the semen with qualified sperm motility, and discarding supernate; freezing liquid is added, and the freezing liquid is fully mixed with the seminal fluid without supernate; sub-packaging the seminal fluid fully mixed with the refrigerating fluid into a plurality of 0.5 mL thin tubes; putting the thin tube filled with the semen into a dark container, and slowly cooling to 4 DEG C for balancing; putting the balanced seminal fluid on a freezing rack at 4 DEG C; the thin tube filled with the semen is placed above the liquid nitrogen surface for fumigation, and then the thin tube is placed in the liquid nitrogen liquid for freezing. The semen is mostly in an environment of 37 DEG C or about 4 DEG C before the liquid nitrogen is utilized, sperm activity cannot be reduced, and freezing of the semen can be realized after the semen is put into the liquid nitrogen liquid (a thin tube filled with the semen is put into the liquid nitrogen liquid), so that sperm activity is guaranteed.

Description

technical field [0001] The invention relates to the technical field of artificial insemination, in particular to a method for freezing semen of a two-humped camel. Background technique [0002] The Bactrian camel is a national-level key protected animal, and it is included in the "Red List of Chinese Species" and the "Red List of Threatened Species of the International Union for Conservation of Nature". At present, there are wild Bactrian camels and domestic Bactrian camels. Populations of wild Bactrian camels continue to decline worldwide due to slower reproduction rates. Domestic Bactrian camels in my country are mainly distributed in the northwest and northern parts of North China. Artificial insemination technology is mainly used to ensure the reproduction speed of domestic Bactrian camels. At present, there is very little disclosure about the semen freezing technology after artificial semen collection in Bactrian camels. The traditional method of freezing semen in Bact...

AI Technical Summary

Problems solved by technology

At present, there are very few disclosures about the semen freezing technology after artificial semen collection of Bactrian camels. The traditional method of semen freezing of Bactrian camels will affect the sperm motility, and there will be a head-to-head phenomenon

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