Short peptide for bacterium resisting, itching relieving, inflammation diminishing, swelling eliminating and scar removing, and its application
A technology of short peptides and scars, applied in the field of biotechnology and medicine, can solve problems such as side effects, scars left by patients, and skin diseases that cannot be cured
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[0027] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.
[0028] The amino acid sequence of the short peptide, Tyr-Leu-Leu-Glu-Ala-Lys or Tyr-Leu-Tyr-Glu-Val-Ala-Arg or Tyr-Leu-Tyr-Glu-Val-Ile-Arg as the main sequence, from Short peptide fragments, short peptide analogs, short peptide derivatives, short peptide variants, etc. that are truncated at any position into 3 or 4 or 5 or 6 amino acids and have the same biological activity as the short peptide have the same biological activity as the short peptide. Efficacy as described in the invention.
[0029] Nucleotides are prepared by...
Embodiment 1
[0049] Antibacterial experimental effect (synthesis of the short peptide involved in the present invention: Tyr-Leu-Tyr-Glu-Val-Ala-Arg)
[0050] The short peptide of the present invention is chemically synthesized by solid-phase Fmoc method. After HPLC purification, the purity exceeds 97%. Staphylococcus aureus is used for antibacterial experiments. That is, first inoculate and activate the slant of the bacteria, place it in a 37°C incubator for 24 hours, and observe the growth of pure Staphylococcus aureus on the slant the next day; for the activated Staphylococcus aureus, take a small amount and 100ml of liquid medium (prepared in 500ml system) , beef extract 2.5g, peptone 7.5g and NaCl 2.5g) in a shaker (37°C, 150r / min) for 12h, after which it can be seen that the culture solution changes from clarification to turbidity. The short peptide involved in the present invention is made into a liquid with a concentration of 0.1 mg / ml, and 100 ml is added to a solid medium (prepar...
Embodiment 2
[0052] Neurodermatitis (synthesis of the short peptide involved in the present invention: Tyr-Leu-Leu-Glu-Ala-Lys)
[0053] The short peptide of the present invention is chemically synthesized by solid-phase Fmoc method. After HPLC purification, the purity exceeds 97%. Staphylococcus aureus is used for antibacterial experiments. That is, first inoculate and activate the slant of the bacteria, place it in a 37°C incubator for 24 hours, and observe the growth of pure Staphylococcus aureus on the slant the next day; for the activated Staphylococcus aureus, take a small amount and 100ml of liquid medium (prepared in 500ml system) , beef extract 2.5g, peptone 7.5g and NaCl 2.5g) in a shaker (37°C, 150r / min) for 12h, after which it can be seen that the culture solution changes from clarification to turbidity. The short peptide involved in the present invention is made into a liquid with a concentration of 0.1 mg / ml, and 100 ml is added to a solid medium (prepared in a 500 ml sys...
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