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A kind of extraction method of heparin sodium crude product

An extraction method, the technology of heparin sodium, applied in the field of biopharmaceutical production, can solve the problems of high cost of pig small intestine, impossibility to improve the purity, and inability to develop rapidly, so as to facilitate large-scale production, reduce the amount of resin used, and be economical. significant effect

Active Publication Date: 2015-08-26
湖南迪博制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to fierce competition, the cost price of pig small intestine remains high, and the purity of the pig lung extraction process cannot be improved due to the traditional and backward salt solution and precipitation technology, making it unable to develop rapidly and causing a lot of waste of resources

Method used

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  • A kind of extraction method of heparin sodium crude product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Take fresh pig lungs or frozen pig lungs after natural thawing, peel off the trachea and fat, and twist them into a paste to obtain pig lung slurry. Take 10kg of pig lung slurry, add 5000ml of deionized water, and then add 15g of sorbic acid Potassium and 15g of hexadecyltrimethylammonium bromide, stirred at 25°C, the stirring speed was less than 50 rpm, once an hour, stirring for 30 minutes each time, stopping for 30 minutes, stirring and reacting for 4 hours, and the first pig was obtained. Lung slurry liquid, in the first pig lung slurry liquid, add the deionized water of 30000ml, get the second pig lung slurry liquid, add 1.8kg sodium chloride again, adjust pH to 9 with sodium hydroxide solution, at 25 Stir at ℃, the stirring speed is less than 50 rpm, stir once per hour, stir for 30 minutes each time, stop for 30 minutes, stir and react for 4 hours, and obtain the pig lung alkaline hydrolyzate;

[0042] 2) Slowly heat the pig lung alkaline hydrolysis solution ob...

Embodiment 2

[0049] 1) Take fresh pig lungs or frozen pig lungs after natural thawing, peel off the trachea and fat, and twist them into a paste to obtain pig lung slurry. Take 10kg of pig lung slurry, add 5000ml of deionized water, and then add 15g of benzoic acid and 15g of sodium dodecylsulfonate, stirred at 25°C, the stirring speed was less than 50 rpm, stirred once per hour, stirred for 30 minutes each time, stopped for 30 minutes, stirred and reacted for 4 hours, and the first porcine lung slurry was obtained Feed liquid, add 30000ml deionized water to the first porcine lung slurry liquid to obtain the second porcine lung slurry liquid, then add 2.0kg sodium chloride, adjust pH to 9 with sodium hydroxide solution, at 25°C Stirring, the stirring speed is less than 50 rpm, stirring once per hour, stirring for 30 minutes each time, stopping for 30 minutes, stirring and reacting for 4 hours, to obtain the pig lung alkaline solution;

[0050] 2) Slowly heat the pig lung alkaline hydrolysi...

experiment example

[0057] Contrast the effective yields of Examples 1 and 2 with the traditional process below. The traditional process refers to the method of removing impurities such as protein without centrifuge separation, and the results are as follows.

[0058] The present invention and traditional process effective yield comparative table

[0059]

[0060] 1. When using trichloroacetic acid method to titrate crude heparin sodium solution, protein impurities will make the solution turbid, and the higher the turbidity, the higher the protein content;

[0061] 2. Many impurities such as protein contained in the crude heparin sodium solution will be preferentially combined with the resin, resulting in an ineffective reaction of the resin, and at the same time increasing the amount of resin and reducing the service life of the resin;

[0062] 3. The extraction method of heparin sodium of the present invention effectively separates heparin sodium and protein impurities, improves potency and ...

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Abstract

The invention relates to an extraction method of a heparin sodium crude product, which comprises the steps that a raw material from a fresh pork lung or a thawy pork lung is minced into a pasty material; a cell activator is added for catalysis; biological enzyme enzymolysis, decontamination, separation and filtering are performed; a solution containing heparin sodium is obtained; resin adsorption, elution, organic solvent precipitation and drying are performed; and the heparin sodium crude product is obtained. The extraction method of the heparin sodium crude product can improve the product quality, can increase an effective yield, can lower the production cost, is suitable for industrial production, reduces waste emission, and has social, environmental and economic benefits.

Description

technical field [0001] The invention relates to a method for extracting crude heparin sodium, belonging to the technical field of biopharmaceutical production. Background technique [0002] Heparin is a natural anticoagulant drug that has the functions of dissolving thrombus and preventing blood coagulation. It is also the compound with the most complex molecular structure known in the world so far. It is widely distributed in mammalian tissues, such as intestinal mucosa, lung, liver, etc. Among them, only the heparin extracted from porcine small intestine mucosa and porcine lung can be used for clinical treatment. [0003] China's pig slaughtering volume accounts for more than 50% of the world, and it has the most abundant heparin raw material resources in the world. my country is the main producer of crude heparin and heparin raw materials in the world, and is also the world's largest exporter of heparin raw materials. It has become an important resource favored by global...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10
Inventor 叶茂南沈迅
Owner 湖南迪博制药有限公司
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