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A measurement method and technology of linear objects, applied in the field of measurement, can solve problems affecting the accuracy of measurement results, troublesome measurement of linear substances of different quantities and different diameters, etc., and achieve high accuracy, simple use, and strong feasibility Effect
Inactive Publication Date: 2015-11-18
NORTHWEST A & F UNIV
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Problems solved by technology
This is very troublesome for measuring linear substances with different amounts and different diameters, and in the process of measuring under a stereo microscope, shaking will cause the linear substances in the suspension to swim, thereby affecting the accuracy of the measurement results
At present, there is no simple multi-scale measurement method for measuring the length of thin wires that are difficult to distinguish with the naked eye
Method used
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Embodiment 1
[0017] Embodiment 1: use the present invention to measure the length of the root system of a plant with a diameter below 1mm.
[0018] a. Set two glass petri dishes with higher light transmittance, wherein the bottom thickness of the upper petri dish 2 is 0.1cm, the bottom thickness of the lower petri dish 1 is 0.15cm, and the inner diameter of the lower petri dish 1 is 14.8cm, The outer diameter is 15.2cm, the inner diameter of the upper petri dish 2 is 14.2cm, and the outer diameter is 14.6cm;
[0019] b. Collect scattered plant roots with a diameter of less than 1mm in the soil through a wet sieve, design a small square with a length of 14 / 11cm, and print it on cellophane with a printer to make cellophane 3;
[0020] c. Add grape seed oil adsorbent 4 dropwise to the upper petri dish 2 and spread it out evenly, then use scissors to cut the printed square cellophane 3 into a circular shape with a diameter of 14.1 cm and spread it on the upper petri dish 2. The cellophane 3 a...
Embodiment 2
[0025] Embodiment 2: Utilize the present invention to measure the length of mycelia of arbuscular mycorrhizal fungi in soil.
[0026] a. Set two glass petri dishes with higher light transmittance, wherein the bottom thickness of the upper petri dish is 0.1cm, the bottom thickness of the lower petri dish 1 is 0.15cm, and the inner diameter of the lower petri dish is 8.8cm, The outer diameter is 9.2cm, the inner diameter of the upper petri dish is 8.2cm, and the outer diameter is 8.6cm;
[0027] b. Obtain the arbuscular mycorrhizal fungus hyphae in the soil by sucking the soil suspension by suction filtration on a filter membrane with a diameter of 8 cm, and dye it with a dye, design a small square length of 7 / 11 cm, and use a printer to print on cellophane On top, make cellophane grids 3;
[0028] c. Add the grape seed oil adsorbent 4 dropwise to the upper petri dish 2 and spread it out evenly, then cut the printed square cellophane 3 into a circle with a diameter of 8.1 cm wi...
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Abstract
The invention discloses a measurement method for the length of thin thread-shaped objects. The technical scheme adopted by the invention is that the measurement method comprises the following steps of setting two glass culture dishes with higher transparency and printing on cellophane to form grid cellophane; adsorbing the grid cellophane to an upper glass culture dish by using an adsorbing agent, dropping a layer of floating carrier agent in a lower glass culture dish and dispersing and placing the thin thread-shaped objects in the lower glass culture dish and enabling the thin thread-shaped objects not to be overlapped; placing the upper upper glass culture dish in the lower upper glass culture dish and counting total cross points of horizontal and vertical grid lines of the thin thread-shaped objects and the grid cellophane; converting by using an improved criss-cross method formula that R is equal to 11 / 14*N*a to obtain the length of the thin thread-shaped objects. According to the measurement method disclosed by the invention, the sizes of the grids are designed by the using printable cellophane and combining specific measurement conditions; meanwhile, by adopting a splint-shaped upper-lower culture dish structure with two layers of light-transmitting glass, the thin thread-shaped objects to be measured do not float in a carrying agent easily and further the measurement result is more accurate. The measurement method has the advantages of simpleness in use, strong feasibility and high accuracy.
Description
technical field [0001] The invention relates to a measuring method, in particular to a measuring method for the length of a fine thread, belonging to the technical field of measurement. Background technique [0002] Measuring the length of the tiny roots of plants and mycelium and other scattered threads with a diameter of less than 1mm is an important part of studying the interaction between plant roots and underground fungi. However, the current traditional root scanning instruments are not accurate enough for the measurement of such linear objects. In order to measure the length of thread-like objects below such diameters, people can only rely on the traditional cross method (author: Newman, E "Amethodofestimatingthetotallengthofrootinasample" "JournalofappliedEcology" 139-145, 1966), and use the improved cross method formula R= 11 / 14×N×a is converted to measure the length of the thin linear object, R is the length of the measured object, N is the number of intersections...
Claims
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Application Information
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