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Immunochromatographic analyzer calibration method

A technology of immunochromatography and calibration methods, which is applied in the direction of analytical materials, instruments, scientific instruments, etc., and can solve problems such as unrepeatable results, loss of the original meaning of instrument determination, and inconsistent measurement results.

Inactive Publication Date: 2014-01-15
王胜 +1
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Problems solved by technology

[0005] Due to the influence of the strength of the signal source, the stability of the sensor, the attenuation of electronic components, and the use of environmental factors, the accuracy of the reading results of the reader is often affected, resulting in large differences in the measurement results between batches and within batches of the instrument, even The results measured by the same instrument at different times are also inconsistent, resulting in unrepeatable results and losing the original meaning of instrument measurement

Method used

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  • Immunochromatographic analyzer calibration method
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Embodiment Construction

[0015] The following describes the implementation of the present invention in conjunction with a typical example of the method.

[0016] like figure 1 Shown is a typical calibration card format that contains a stepped pattern printed in 5 different shades of gray from light to dark.

[0017] like figure 2 Shown is an immunochromatography analyzer calibration procedure flow, including the following steps:

[0018] Step 1: Use a standard reader to measure the target values ​​of the 5 gradients in the calibration card (1) from low to high through the process of data acquisition (3): T1, T2, T3, T4, T5, and save this information to the calibration data (5).

[0019] Step 2: Use the calibrated reading instrument to determine the measured values ​​of the five gradients through the data acquisition (3) process: X1, X2, X3, X4, and X5.

[0020] Step 3: Send the two sets of data in steps 1 and 2 to the data processing (4) module, take the measured value (X1-X5) as the abscissa, an...

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Abstract

The invention discloses an immunochromatographic analyzer calibration method comprising characteristic steps that: 1, calibration cards are prepared in batches, wherein specific information zones can be generated through direct printing, immunization or physiochemical methods; 2, signal intensity at the specific zone is read by using a standard device; the signal intensity is digitized, and is stored as a standard result; 3, the calibration card is re-read by using a calibrated device; the data is compared with that of the standard device; data fitting is carried out, such that the parameters of the calibrated device are corrected, and calibration is finished. The method has significant effect that: calibrated parameters are used for correcting the measuring result of a sample, such that a standardized measuring result which is not influenced by exterior facts is obtained.

Description

technical field [0001] The invention belongs to the field of qualitative or quantitative detection of immunochromatography cards or test strips, and relates to a method for improving the accuracy of detection results of immunochromatography cards or test strips. Background technique [0002] Immunochromatography is a rapid detection technology that has emerged rapidly in recent years. Colloidal gold, latex particles, magnetic beads, fluorescein, etc. are often used as tracer markers to detect antigen-antibody reactions. This technology has the advantages of simple and fast operation, high sensitivity, strong specificity, good stability, accurate results, and easy judgment. [0003] With the advancement of technology, the traditional method of observing the results with the naked eye is gradually being replaced by instrument reading. Using the instrument to read the results avoids the influence of some human subjective factors to a certain extent, so that the accuracy of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 王胜刘志刚
Owner 王胜
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