Protein chip capable of quickly providing early-stage depression pre-warning

A protein chip and depression technology, applied in the field of protein chips, can solve the problems of the popularization, high price, and expensive instruments that are difficult to early warning detection of depression, and achieve the effect of being suitable for popularization and use, high accuracy and high specificity

Inactive Publication Date: 2014-01-15
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the traditional protein chip technology is complicated to operate, requires expensive instruments, and is expensive. It is difficult to popularize early warning detection of depression, especially in grassroots medical units and scientific research institutions.
However, the traditi

Method used

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  • Protein chip capable of quickly providing early-stage depression pre-warning
  • Protein chip capable of quickly providing early-stage depression pre-warning
  • Protein chip capable of quickly providing early-stage depression pre-warning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Meta analysis to find out the pro-inflammatory cytokines closely related to the onset of depression in the population

[0035] Meta analysis is "meta-analysis", which is defined as "The statistical analysis of large collection of analysis results from individual studies for the purpose of integrating the findings." A type of statistical method. The steps are as follows:

[0036] ①A question that needs to be addressed: "Can allergic inflammation / pro-inflammatory cytokines mediate depression";

[0037] ② Comprehensive collection of allergy and depression-related epidemiological research literature based on PubMed network;

[0038] ③Select and extract the collected documents (including result data, charts, etc.) with the help of established inclusion and exclusion criteria;

[0039] Search on MEDLINE with depression, allergy, cytokines, asthma, mental disorder as the subject headings. After excluding animal experiments, reviews, reviews, and articles that cannot be extra...

Embodiment 2

[0053] Example 2 Epidemiological investigation and study of depression

[0054] In case-control investigation, a total of 100 hospitalized cases of depression in the Department of Psychiatry of Donghu Hospital (college students) and a control of 120 healthy students from Central China Normal University collected blood samples of 5 ml each for the determination of the above-mentioned serum protein content, combined with the diagnosis of depression The procedures for obtaining and preprocessing blood samples include: organizing medical professional practitioners and nurses to set up a sampling team, asking the respondents to fill in an informed consent form, and each responding to the hospital one morning during the investigation. The blood is drawn by a nurse practitioner (the blood collection tube has been pre-added with anticoagulant), placed in a refrigerator at 4°C, and ELISA testing will be performed intensively within one month.

Embodiment 3

[0055] Example 3 ELISA to detect the content of serum protein (TNF-α, IL-1, IL-4)

[0056] Enzyme linked immunosorbent assay (enzyme linked immunosorbent assay, ELISA) used for antigen localization enzyme-labeled antibody technology has developed into a method for the determination of trace substances in liquid specimens. The above-mentioned serum levels of TNF-α, IL-1, and IL-4 were detected using standard ELISA detection techniques. It uses the specific reaction of the antigen and the antibody to connect the test substance with the enzyme, and then the enzyme and the substrate produce a color reaction for quantitative determination. In this assay method, there are three necessary reagents: solid-phase antigen or antibody (immunosorbent), enzyme-labeled antigen or antibody (marker), and substrate for enzyme action (color reagent). When measuring, the antigen (antibody) is first bound to the solid carrier, but still retains its immunological activity, and then a conjugate (label...

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Abstract

The invention discloses a protein chip capable of quickly providing early-stage depression pre-warning, and belongs to the field of biotechnology. The protein chip is a solid-phase microwell plate coated with the antibodies of TNF-alpha (tumor necrosis factor), IL-1 (interleukin-1), and IL-4 (interleukin-4). Reagents matched with the protein chip comprise the detecting antibodies of TNF-alpha, IL-1, and IL-4 labeled with biotin, and HRP (horse reddish peroxidase) labeled with avidin, and a substrate on which the HRP acts. An ELISA (enzyme linked immunosorbent assay) method is used for detecting the expression conditions of TNF-alpha, IL-1, and IL-4 in serum of an tested individual and normal people, if the expression quantity of TNF-alpha, IL-1, and IL-4 in the serum of the tested individual is obviously higher than that in the serum of the normal people, then the tested individual can be judged to have an early-stage depression propensity. The protein chip is used for providing early-stage depression pre-warning through protein levels. The protein chip has high sensitivity and specificity, is easy and quick to operate, good in stability and low in cost, and can be widely applied to the early-stage depression pre-warning.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to a protein chip for rapid early warning of depression. Background technique [0002] Depression (DEP) is a mental illness that seriously affects people's physical and mental health. Depression is an affective mental disorder, and its pathogenesis is very complicated. It is a syndrome with mood disorders as the main symptoms caused by many reasons. Modern medical research shows that mental health and physical conditions are closely related. According to reports in the literature, some immune cells such as monocytes, mast cells, T lymphocytes and other nuclear cytokines play an important role in the onset of depression. [0003] With the development of society, the pace of life has accelerated, competition pressure has increased, and changes in population and family structure have led to an increase in the prevalence of severe mental illness. Mental health has become a prominent public health ...

Claims

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Application Information

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IPC IPC(8): G01N33/96
CPCG01N33/54306G01N33/6893G01N2800/304
Inventor 刘旭东刘英帅江明頔张玉超覃萍杨旭
Owner HUAZHONG NORMAL UNIV
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