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Nucleotide variation ratio quantitative analyzing method based on DNA sequencing peak diagram

A technology of DNA sequencing and quantitative analysis, applied in special data processing applications, instruments, electronic digital data processing, etc., can solve problems such as complex operation procedures, harsh experimental conditions, and low accuracy, and achieve good application prospects and low programming workload Small, Inexpensive Effects

Inactive Publication Date: 2014-02-05
SOUTHERN MEDICAL UNIVERSITY
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Problems solved by technology

Such as CN101250581B A kind of displacement amplification method for detecting the variation of hepatitis B virus P gene YMDD discloses the variation of detecting hepatitis B virus (HBV) P gene YMDD by displacement amplification method, the method is suitable for the qualitative analysis of single base variation, Although it can be combined with SYBR Green dye or fluorescent molecular probe for quantitative analysis of a single gene to be tested, the operation process is complicated, the experimental conditions are harsh, the accuracy is not high, and the cost is high.

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  • Nucleotide variation ratio quantitative analyzing method based on DNA sequencing peak diagram
  • Nucleotide variation ratio quantitative analyzing method based on DNA sequencing peak diagram
  • Nucleotide variation ratio quantitative analyzing method based on DNA sequencing peak diagram

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Embodiment 1

[0031] 1. Open the peak shape diagram of DNA sequencing

[0032] Patients with chronic hepatitis B are treated with oral nucleotide analogues (such as lamivudine) for a long time, and HBV will undergo base mutations to make it drug-resistant. For example figure 1 Open the HBV DNA sequencing peak shape chart with Chromas software, figure 1 The middle arrow has a double-peak pattern, and the peak pattern of the pointed base C produces another peak pattern of the base A, which is produced by the mutation of the base C. The purpose of developing the base variation ratio quantitative identification software is to calculate the area ratio under different curves in the DNA profile at the same base site, so as to obtain the ratio of the base variation ratio. The method of calculating the area is to find the number of pixels under the peak-shaped curve. In Chromas, no matter how the display scale in the XY direction of the image is adjusted, although the area under each peak-shaped c...

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Abstract

The invention discloses a nucleotide variation ratio quantitative analyzing method based on a DNA sequencing peak diagram. The method includes according to a DNA sequencing diagram, for nucleotide variation points with specific meanings, calculating the sum of pixel points under each peak curve in the double-peak image of the points, and acquiring the area of the peak curve; then calculating the area ratio of the two peak images, and acquiring the specific ratio of the DNA nucleotide variation.

Description

technical field [0001] The invention relates to a method for quantitatively analyzing the ratio of DNA base variation, in particular to a method for quantitatively analyzing the ratio of base variation based on DNA sequencing peak shape. Background technique [0002] At present, with regard to base variation, most of them are qualitative analysis of variation, and quantitative analysis of variation ratio has not been reported yet. Such as CN101250581B A kind of displacement amplification method for detecting the variation of hepatitis B virus P gene YMDD discloses the variation of detecting hepatitis B virus (HBV) P gene YMDD by displacement amplification method, the method is suitable for the qualitative analysis of single base variation, Although it can be combined with SYBR Green dye or fluorescent molecular probe for quantitative analysis of a single gene to be tested, the operation process is complicated, the experimental conditions are harsh, the accuracy is not high, ...

Claims

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Application Information

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IPC IPC(8): G06F19/26G06F19/22C12Q1/68G16B45/00
Inventor 刘国庆钱俊
Owner SOUTHERN MEDICAL UNIVERSITY
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