Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves

A technology for triterpene ellagitannins and Mucuna pruriens, which is applied in the field of extracting active ingredients from plants, can solve problems such as unsatisfactory yield, difficult industrialization, complicated processes, etc., achieves simple operation, and improves product yield and purity. high effect

Inactive Publication Date: 2014-02-26
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the above documents can be separated and refined to obtain triterpene ellagitannins, this method uses Sephadex LH-20, HP20SS CC and Chromatorex ODS multiple chromatography,

Method used

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  • Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves
  • Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves
  • Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Take 6.0 kg of fresh leaves of Mucuna pruriens, chop finely, add 80% acetone solution 5 times the weight of the raw material, extract at room temperature for 24 hours, filter, add 80% acetone solution 5 times the weight of the raw material to the filter residue, extract at room temperature for 12 hours, Filtrate and combine the filtrates to obtain the extract;

[0022] 2) When the extract is recovered from acetone under reduced pressure until complete, the chlorophyll and wax are removed by filtration to obtain the filtrate;

[0023] 3) Apply D101 macroporous resin chromatography to the filtrate obtained in step 2), wash the column with 4 times the resin volume of water, then elute with 4 times the resin volume of 50% ethanol, and then elute with 80% ethanol until there is no phenolic reaction (2wt%FeCl 3 For aqueous solution detection, the amount of 80% ethanol is about 7 times the volume of the resin), and the 80% ethanol eluate is collected;

[0024] 4) The coll...

Embodiment 2

[0038] 1) Take 6.1 kg of fresh leaves of Mucuna pruriens, chop or pulverize, add 80% acetone solution 5 times the weight of the raw material, reflux extraction for 2 hours, filter, add 80% acetone solution 5 times the weight of the raw material to the filter residue, and reflux extraction for 2 hours, Filtrate and combine the filtrates to obtain the extract;

[0039] 2) When the extract is recovered from acetone under reduced pressure until complete, the chlorophyll and wax are removed by filtration to obtain the filtrate;

[0040] 3) HP-20 macroporous resin chromatography on the filtrate obtained in step 2), washing the column with water with 4 times the volume of the resin, then eluting with 30% ethanol with 4 times the volume of the resin, and then eluting with 90% ethanol until there is no phenol Sexual reaction (2wt%FeCl 3 For aqueous solution detection, the amount of 90% ethanol is about 6 times the volume of the resin), and the 90% ethanol eluate is collected;

[0041...

Embodiment 3

[0043] 1) Take 6.5kg of fresh leaves of Mucuna pruriens, chop finely, add 90% acetone solution of 10 times the weight of raw materials, extract at room temperature for 24 hours, filter, add 90% acetone solution of 5 times the weight of raw materials to the filter residue, extract at room temperature for 10 hours, Filtrate and combine the filtrates to obtain the extract;

[0044] 2) When the extract is recovered from acetone under reduced pressure until complete, the chlorophyll and wax are removed by filtration to obtain the filtrate;

[0045] 3) Apply the D-4020 macroporous resin chromatography to the filtrate obtained in step 2), wash the column with 6 times the volume of the resin, then elute with 50% methanol of 5 times the volume of the resin, and then elute with 80% methanol until there is no phenol Sexual reaction (2wt%FeCl 3 For aqueous solution detection, the amount of 80% methanol used is about 7 times the volume of the resin), and the 80% methanol eluate is collect...

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Abstract

The invention discloses a method of extracting and separating a triterpenoid ellagitannin compound from castanopsis fissa leaves. The method comprises following steps of: 1) extracting the castanopsis fissa leaves used as a raw material by using acetone to obtain an extract; 2) recovering the acetone in the extract and filtering to obtain a filtrate; 3) passing the filtrate through macroporous resin, washing with water, eluting by using 20-50% in volume of an organic solvent, then eluting by using 70-100% in volume of the organic solvent until the eluate has no reactions of phenols, and collecting the eluate of 70-100% in volume of the organic solvent; and 4) recovering the solvent in the eluate under reduced pressure until a large quantity of crystals precipitate, cooling, permitting the eluate to stand and separating the crystals. According to the method, the macroporous resin is used for chromatography and the special eluting technology is combined, and therefore the cost is reduced, the product yield which can reach over 0.37% is improved at the same time, and the purity is high. The method is simple in operation and prone to industrialization.

Description

technical field [0001] The invention relates to the technical field of extraction of effective components in plants, in particular to a method for extracting and separating triterpene ellagitannins from leaves of Cone pruriens. Background technique [0002] Castanopsis fissa (Champ.ex Benth.) Rehd.et Wils.) is a plant of the genus Cone in the family Fagaceae, also known as Castanopsis fissa, Champ. It is widely distributed in South China. It is an excellent fast-growing and high-yield native tree species in South my country. It has very rich plant resources. It is widely used in the treatment of cardiovascular and cerebrovascular diseases, hemostasis, diarrhea, and chronic ulcers among the people. The chemical composition of the plant has been studied in detail in the literature (Phytochemistry, 2011, 72(16): 2006-2014). In the literature, 80% acetone aqueous solution was used to extract, and Sephadex LH-20 (10-100% methanol solution washed elution), HP20SS CC (60%, 70%, 80...

Claims

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Application Information

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IPC IPC(8): C07J71/00
Inventor 沈晓琳黄永林李典鹏田中隆陈月圆刘金磊卢凤来
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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