Tissue culture method for hemerocallis sempervirens araki

A tissue culture and Hemerocallis technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that hinder the large-scale application of Evergreen Hemerocallis, and achieve the effect of facilitating multiplication and rapid supply

CN103609444AActive Publication Date: 2014-03-05ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-03-05

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Abstract

The invention discloses a tissue culture method for hemerocallis sempervirens araki. The tissue culture method comprises the following steps: (1) washing taken stems of hemerocallis sempervirens araki plants by water; (2) after the stems are disinfected, picking off stem tips and inoculating the stem tips into an induction aseptic seedling culture medium to be cultured; (3) after aseptic seedlings in the step (2) grow to 1.0cm-2.0cm, cutting to obtain plantlets I; inoculating the plantlets I into an adventitious bud induction culture medium to be cultured; (4) after adventitious buds induced from the plantlets I grow to 1.0cm-3.0cm, cutting by taking 2-5 strains as a cluster to obtain clustered plantlets; inoculating the clustered plantlets to a proliferation culture medium to be cultured; (5) after the adventitious buds on the clustered plantlets grow to 3.0cm-6.0cm, cutting to obtain plantlets III; inoculating the plantlets III into a rooting culture medium to be cultured; and (6) when at least five roots with the length of more than 2cm grow in the plantlets III, so as to obtain germchits capable of being planted out of a bottle.
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Description

technical field

[0001] The invention relates to a tissue culture method of Hemerocallis evergreen. Background technique

[0002] Evergreen daylily has the characteristics of evergreen in four seasons, long flowering period, strong disease resistance, cold resistance, and semi-shade tolerance. It is a popular plant for greening and gardening. In addition, the root of Hemerocallis evergreen can be used as medicine, which has diuretic and cooling blood effects, and is used to treat edema, dysuria, stranguria, leukorrhea, jaundice, blood in the stool, metrorrhagia and other diseases. Anthraquinones, dihydrofuran-γ-lactams and other compounds isolated from Hemerocallis evergreen have antibacterial, anticancer, insecticidal and other activities. According to the production survey, Evergreen Hemerocallis seldom grows under natural conditions and can reproduce by seeding. Generally, the "division method" is used for reproduction, and the annual reproduction coefficient is generally...

Examples

Embodiment 1

[0035] Embodiment 1, a kind of tissue culture method of Hemerocallis evergreen, carries out following steps successively:

[0036] 1) Take an evergreen Hemerocallis plant that grows robustly and is free from diseases and insect pests, peel off the outer 1-2 layers of old leaves, take the 1-2cm long stem section from the tip, put it in a gauze bag and wash it with running water for 0.5-1 Hour.

[0037] 2) After the stems washed with the above-mentioned running water are routinely disinfected (that is, 0.1% w / v HgCl 2 After 6 to 10 minutes of treatment, rinse with sterile water 5 to 8 times, and then blot dry with sterile filter paper), peel off the shoot tip with a length of 3 to 5 mm, and inoculate it on the culture medium for inducing sterile seedlings; The conditions are: 16 hours of light, light intensity 30 ~ 40μmol m -2 ·s -1 , the temperature is 27±1°C; 8 hours of dark cultivation, the temperature is 21±1°C; the above-mentioned light and dark cultivation are carried o...