Preparation method and application of competitive type aptamer sensor for detecting oxytetracycline
An aptamer sensor, oxytetracycline technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of expensive, time-consuming and expensive, and achieve the effect of fast detection speed, high sensitivity, and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] A method for preparing a competitive aptasensor for detecting oxytetracycline (OTC), the steps are as follows:
[0027] (1) Drop 20 μL of GO-PANI nanocomposite solution onto the working surface of the glassy carbon electrode and dry at room temperature;
[0028] (2) Immerse the (1) electrode in HAuCl 4 (1 mg / mL) working solution, the gold nanoparticles were reduced to (1) the electrode surface by electroreduction, the initial potential was -0.2 V, and the reduction time was 20, 40, 60, 80, 100, 120, 140, 160, 180 S, room temperature to dry.
[0029] (3) Ag / AgCl was used as the reference electrode, the Pt electrode was used as the counter electrode, the sensor platform obtained in (2) was used as the working electrode, and the K 3 [Fe(CN) 6 ] is the working bottom liquid, connected to the electrochemical workstation, and the current response is detected by cyclic voltammetry. The potential was set from -0.2 to +0.6 V and the scan rate was 50 mV / s.
Embodiment 2
[0031] A method for preparing a competitive aptasensor for detecting oxytetracycline (OTC), the steps are as follows:
[0032] (1) Drop 20 μL of GO-PANI nanocomposite solution onto the working surface of the glassy carbon electrode and dry at room temperature;
[0033] (2) Dip (1) electrodes into different concentrations (1×10 -4 , 1×10 -3 , 1×10 -2 , 1×10 -1 , 1, 10 mg / mL) of HAuCl 4 In the working solution, the gold nanoparticles were reduced to (1) the electrode surface by means of electroreduction, the initial potential was -0.2 V, the reduction time was 120 s, and they were dried at room temperature.
[0034] (3) Ag / AgCl was used as the reference electrode, the Pt electrode was used as the counter electrode, the sensor platform obtained in (2) was used as the working electrode, and the K 3 [Fe(CN) 6 ] is the working bottom liquid, connected to the electrochemical workstation, and the current response is detected by cyclic voltammetry. The potential was set from -0...
Embodiment 3
[0036] A method for preparing a competitive aptasensor for detecting oxytetracycline (OTC), the steps are as follows:
[0037] (1) Drop 20 μL of GO-PANI nanocomposite solution onto the working surface of the glassy carbon electrode and dry at room temperature;
[0038] (2) Immerse the (1) electrode in HAuCl 4 (1 mg / mL) working solution, the gold nanoparticles were reduced to (1) the electrode surface by electroreduction, the initial potential was -0.2 V, the reduction time was 120 s, and dried at room temperature.
[0039] (3) Drop 20 μL of different concentrations (1×10 -5 , 1×10 -4 , 1×10 -3 , 1×10 -2 , 1×10 -1 mg / L) OTC-BSA onto the electrode surface, after drying, drop BSA to block non-specific binding sites;
[0040](4) Drop 20 μL of the mixture of HRP-Apt and OTC to be tested onto the surface of the electrode, rinse and dry after 30 minutes;
[0041] (5) Using Ag / AgCl as the reference electrode, using the Pt electrode as the counter electrode, using the sensor p...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com