Bacillus megaterium capable of dissolving microcystis aeruginosa and application thereof

A technology of Bacillus megaterium, Microcystis aeruginosa, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc.

CN103667111AActive Publication Date: 2014-03-26LIAONING UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-03-26

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Abstract

The invention relates to bacillus megaterium capable of dissolving microcystis aeruginosa and an application thereof. The bacterial strain A0 can be used for dissolving the microcystis aeruginosa. When the adding amount is 7.5%, the dissolving function is very obvious in the 6th day of cocultivation, the amount of frustule is 66.7% lower than the matched group and the content of frustule chlorophyll alpha is 35.2% lower than the matched group in the 6th day.
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Description

technical field

[0001] The present invention relates to a bacterial strain screened from eutrophic water body with algicidal effect, the bacterial strain is effective to the main algae causing eutrophication of water body—Microcystis aeruginosa ( Microcystisaeruginosa ) has a dissolving effect.

[0002] A kind of bacillus megaterium that Microcystis aeruginosa is called by the present invention is named after: bacillus megaterium ( Bacillus megaterium ) A 0 , has been preserved in the China Center for Type Culture Collection on September 27, 2013, the address is: Wuhan University, Wuhan, China, and the number is CCTCC NO: M 2013452.

[0003] Background technique

[0004] In recent years, with the improvement of industrialization, the acceleration of urbanization and the continuous increase of population, human activities have become more frequent and profoundly affecting the water environment. The accumulation of nitrogen and phosphorus in the water body reaches a certa...

Examples

Embodiment 1

[0066] Embodiment 1 The method for dissolving Microcystis aeruginosa

[0067] (1) Strain A 0 cultivation

[0068] Medium formula: distilled water 1000mL, beef extract 3g, peptone 10g, NaCl 5g, pH value 7.0~7.2.

[0069] Method: Put 50mL of prepared culture medium into nine 250mL Erlenmeyer flasks, sterilize at 121°C for 20min, cool at room temperature, inoculate strain A with 5% (v / v) inoculum 0 Put the seed liquid into each triangular flask, then put it into a constant temperature oscillator to vibrate, the rotation speed is 160r / min, the temperature is 30°C, take out a bottle every three hours, observe and count under the microscope with a hemocytometer, Calculate the bacterial density, measure the absorbance at 660nm wavelength at the same time, draw the growth curve with time as the abscissa, bacterial density and OD660 as the ordinate. see results image 3 .

[0070] From image 3 It can be seen that 0~3h is the lag phase of bacterial growth, 3~9h is the logarithm...