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siRNA for FOXC1 gene expression inhibition, and application thereof

A gene expression and base technology, applied in the field of molecular biology, can solve problems such as changes in the expression of molecular markers, high expression of FOXC1 indicating poor prognosis, etc.

Active Publication Date: 2014-03-26
FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

In breast cancer, endometrial cancer and other studies, it was found that FOXC1 can promote the proliferation, invasion and migration of cancer cells. These studies also pointed out that the role of FOXC1 in promoting cancer cell invasion and migration may be through participating in EMT (epithelial-mesenchymal transition) , resulting in changes in the expression of EMT-related molecular markers, and the high expression of FOXC1 indicates a poor prognosis

Method used

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  • siRNA for FOXC1 gene expression inhibition, and application thereof
  • siRNA for FOXC1 gene expression inhibition, and application thereof
  • siRNA for FOXC1 gene expression inhibition, and application thereof

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with embodiment.

[0035] 1. Expression of FOXC1mRNA in laryngeal squamous cell carcinoma tissues and corresponding normal paracancerous mucosal tissues

[0036] (1) Specimen acquisition

[0037] Specimens were taken from first-diagnosed and operated patients in the First Hospital of Shanxi Medical University, specifically 20 laryngeal squamous cell carcinoma tissues and normal mucosal control tissues adjacent to the same patient; the specimens were packaged and frozen in liquid nitrogen within 15 minutes after separation; pathology It was confirmed that the obtained specimens were laryngeal squamous cell carcinoma, and no cancer was found in the normal mucosa adjacent to the cancer; the tumor staging (TNM) adopted the UICC2012 version; the pathological classification adopted the WHO standard; this experiment was approved by the Scientific Research Ethics Committee of Shanxi Medical University; See Ta...

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Abstract

The present invention discloses a siRNA molecule for human FOXC1 gene expression inhibition, and an application thereof, and belongs to the field of molecular biology. The siRNA has the following sequence that: the sense strand is 5'-CGGACAAGAAGATCACCCTtt-3', and the antisense strand is 5'-AGGGTGATCTTCTTGTCCGtt-3'. According to the present invention, the siRNA can be provided for effectively inhibiting human FOXC1 gene expression, significantly inhibiting cell proliferation and clone capacity and cell migration and invasion capacity of larynx squamous cell carcinoma cell lines such as Hep-2 and TU-177, and preparing FOXC1-targeting anti-larynx cancer drugs.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to an siRNA molecule for inhibiting human FOXC1 gene expression and application thereof. Background technique [0002] Laryngeal cancer is a malignant tumor that occurs in the epithelium of the laryngeal mucosa. It usually occurs in men aged 50 to 60. It is the second most common primary malignant tumor in the head and neck. The pathological type is mainly squamous cell carcinoma, accounting for about 90%. above. [0003] Laryngeal cancer originates from the epithelium of the laryngeal mucosa and ranks second among the most common malignant tumors in the head and neck, with a high mortality rate. In some provinces of my country, the incidence rate is 1.5-3.4 / 100,000 people, and it has been increasing in the past 20 years and has a younger trend. Due to the limited anatomical structure of the laryngeal cavity and rich lymphoid tissue, invasion and metastasis have become a significant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00
Inventor 高伟张春明王斌全卢岩贺小玲温树信皇甫辉
Owner FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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