Two-stage dissolved oxygen control method for increasing pleocidin output

Abstract

The invention provides a two-stage dissolved oxygen control method for increasing pleocidin output. The method is characterized in that the volumetric mass transfer coefficient kLa of oxygen is adjusted and controlled by adjusting throughput and stirring speed at the beginning of fermentation of saccharopolyspora spinosa in the process of preparing saccharopolyspora spinosa fermentation liquor, thus the initial oxygen volumetric mass transfer coefficient kLa can be controlled to be 100-130h<-1> and the accumulation of biomass of saccharopolyspora spinosa is promoted; then the volumetric mass transfer coefficient kLa of oxygen is adjusted and controlled by adjusting throughput and stirring speed when the fermentation enters the final cell growth exponential phase, and thus the initial oxygen volumetric mass transfer coefficient kLa can be controlled to be 60-92h<-1> and the synthesis and accumulation of pleocidin are promoted. According to the method, the two-stage dissolved oxygen control is performed, which obviously increases the pleocidin output and shortened the fermentation cycle. The two-stage dissolved oxygen control and fermentation method is easily realized and has a high application value.

Description

technical field

[0001] The invention relates to the fields of fermentation engineering and biopharmaceuticals, in particular to the application of dissolved oxygen control technology in the optimization of fermentation process, specifically a two-stage dissolved oxygen control method for increasing spinosad production. Background technique

[0002] Spinosyn (spinosad), also known as spinosad or spinosad, is an intracellular secondary metabolite produced by the soil actinomycete Saccharopolyspora spinosa after aerobic fermentation. The active ingredients are spinosyn component A (spinosyn A) component D (spinosyn D). Spinosyn is a naturally occurring antibiotic that has good control effects on pests such as Lepidoptera and Diptera. Spinosyn has rapid contact and ingestion toxicity to insects, and stimulates the nervous system of insects, resulting in non-functional muscles. Contraction, collapse, with tremors and paralysis. Compared with common insecticides, spinosad has bo...

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