Method for detecting plurality of residual pesticides in tobacco simultaneously

A pesticide residue and tobacco technology, applied in the field of detection of 92 kinds of pesticide residues in tobacco, can solve the problems of loss of target substances and time-consuming, and achieve the effect of reducing dosage, simplifying pretreatment process and improving extraction efficiency.

Active Publication Date: 2015-03-25
ZHENGZHOU TOBACCO RES INST OF CNTC
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AI Technical Summary

Problems solved by technology

However, these methods divide the extraction and purification into two steps, which is not only time-consuming, but also easily causes the loss of the target substance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] 1. Instruments and reagents:

[0026] ASE-200 accelerated solvent extraction instrument (DIONEX, USA), with 33mL extraction cell; Agilent 1200 high performance liquid chromatography (Agilent, USA); AB API4000 triple quadrupole tandem mass spectrometer, with electrospray (ESI) ion source ( American AB Company).

[0027] Acetonitrile, ethyl acetate, acetone (all chromatographically pure, China Dima Technology Company); Florisil (pesticide residue grade, 140-250 μm, China Dima Technology Company); formic acid (chromatographically pure, 96%, TEDIA, USA) diatomite (0.02-0.1 mm, Merck, Germany); graphitized carbon black GCB (Agilent, USA); N-propylethylenediamine (PSA) adsorbent, carbon 18 (C18) adsorbent ( Both are 40-63 μm, China Anpu Company); Pesticide Standards (Germany Dr.Ehrenstorfer Company and China Bailingwei Company).

[0028] 2. Preparation of standard solution: Take an appropriate amount of each pesticide standard, prepare standard working solutions with 9 conc...

example 2

[0041] As described in Example 1, a flue-cured tobacco leaf sample was selected for detection, and among 92 kinds of pesticide targets, 5 kinds of pesticides were detected. The specific detection results are shown in Table 3.

[0042] Table 3 Pesticide residue detection results of samples

[0043] serial number Chinese name English name Detection concentration (mg / kg) 1 Carbendazim Carbendazim 0.15 2 Rice blast spirit Isoprothiolane 0.58 3 Metalaxyl Metalaxyl 0.01 4 Pendimethalin Pendimethalin 0.03 5 Triadimefon Triadimefon 0.04

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Abstract

The invention provides a method for detecting a plurality of residual pesticides in tobacco simultaneously. The method is characterized by carrying out sample pretreatment by an ASE (accelerated solvent extraction) device so that extraction and purification are realized through one step, wherein a florisil and graphitized carbon black (GCB) mixture is added to an accelerated solvent extraction tank at the bottom of which a filter membrane is arranged; next, a mixed sample, which is a mixture of powdered tobacco and kieselguhr, is added to the accelerated solvent extraction tank; in succession, an interior label is added and the unoccupied space above the extraction tank is fully filled with the kieselguhr; after extraction is finished, the extracts are mixed evenly and filtered through a 0.22-micron organic phase filter membrane; finally, liquid chromatography-tandem mass spectrum (LC-MS / MS) detection is carried out. The method provided by the invention is capable of completing the extraction and purification of the sample through one step so that the pretreatment process is greatly simplified; the detection method is high in automation degree, simple to operate, high in sensitivity and accuracy, and good in repeatability; the method is capable of detecting as many as 92 residual pesticides in the tobacco simultaneously, and meets the requirements of a multiple-residual pesticide analysis method.

Description

technical field [0001] The invention belongs to the technical field of detection of multiple pesticide residues in tobacco, and mainly relates to the detection technology of 92 kinds of pesticide residues in tobacco. Background technique [0002] In the process of tobacco planting and storage, pesticide residues are unavoidable. As people pay more and more attention to food safety issues, tobacco, as a kind of smoking food, has also received more and more attention on its pesticide residues as an important part of smoking safety. Therefore, it is of great significance to detect pesticide residues in tobacco. Multi-pesticide residue detection method is the development trend of pesticide residue detection. [0003] Accelerated Solvent Extraction (ASE) is a method for extracting target substances in solid and semi-solid samples at relatively high temperature and pressure. It has the advantages of less solvent consumption, short extraction time, and high degree of automation. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 胡斌艾丹刘惠民潘立宁陈黎王冰谢复炜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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