Method for quickly and simply screening and evaluating marine antifouling compound

Abstract

The invention discloses a method for quickly and simply screening and evaluating a marine antifouling compound. The method comprises the following steps: by using marine bacteria as a tested organism, adding a gelatinizer into a bacterial liquid culture medium, dissolving by heating, cooling, uniformly dispersing the tested compound in the gel solution, uniformly coating the gel solution containing the tested compound on a glass plate to prepare a gel test plate; and putting the gel test plate in seawater in which the tested organism is inoculated, carrying out continuous culture, taking out the gel test plate, rinsing, diluting to the specified volume in a measuring flask, testing the OD (optical density) value or absorbance value, commuting the OD value or absorbance value into antibacterial rate, and comparing the magnitude of the antibacterial rate to evaluate the antifouling property of the tested compound. The evaluation method is simple to operate and easy to implement, has the advantage of short evaluation period, uses the marine bacteria as the tested organism, and can be used for screening the antifouling compounds in the research and development process of marine antifouling paints.

Description

technical field [0001] The invention relates to a rapid screening and evaluation method, in particular to a rapid and simple screening and evaluation method for marine antifouling agents. Background technique [0002] The problem of marine biofouling restricts the development and utilization of marine resources by human beings and brings huge economic losses. At present, the most commonly used method to prevent marine biofouling is to apply antifouling paint. Antifouling agent is one of the most important effective ingredients in antifouling coatings to prevent marine biofouling. Screening and evaluating antifouling agents is an indispensable step in the research and development of antifouling coatings. Improving and improving the evaluation method of antifouling performance is of great significance to the development of antifouling coatings. The national standard GB / T5370-2007 "Shoal Sea Immersion Test Method for Antifouling Paint Samples" provides an evaluation method fo...

AI Technical Summary

Problems solved by technology

The national standard GB / T5370-2007 "Shoal Sea Immersion Test Method for Antifouling Paint Samples" provides an evaluation method for antifouling coatings. After the antifouling agent is formulated into antifouling coatings, it can also be used to evaluate the antifouling coatings in the national standard GB / T5370-2007. The antifouling performance of the stain agent, but the evaluation period is long

Chinese author Tian Bin used three strains of Staphylococcus aureus, Escherichia coli and Saccharomyces alcohol as test organisms to evaluate the antifouling performance of antifouling agents in his master's thesis "Research on Biological Performance of Marine Antifouling Coatings" in 2005, but Since the tested organisms are not typical marine bacteria and cannot represent marine species, the representativeness of the evaluation results is often questioned

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