Method for detecting biological ultra-weak photon radiation spectrum
A technology of photon radiation and spectrum, which is applied in the detection of biological ultra-weak photon radiation spectrum, which can solve the problems of weak biological ultra-weak luminous intensity and difficulty in measuring spectrum by instruments
Inactive Publication Date: 2014-04-16
XIAN UNIV OF TECH
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[0004] The purpose of the present invention is to provide a method for detecting the spectrum of biological ultra-weak photon radiation, which solves the problem that existing instruments are difficult to measure the spectrum of biological ultra-weak luminescence because the intensity is too weak
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[0058] Take 20 mung beans that have germinated for 48 hours, put them in a sample cup, put them in a dark room, and measure the delayed luminescence with wavelengths of 460nm, 475nm, 490nm, 505nm, 520nm, 535nm, 555nm, 575nm, 590nm, 605nm, and 620nm according to the above steps curves, such as figure 2 shown. The spectral curve obtained by fitting according to the measured results is as follows: image 3 shown.
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The invention discloses a method for detecting a biological ultra-weak photon radiation spectrum. The method includes the steps firstly, delayed luminescence curve data of a living biological sample on different wave lengths are collected accurately, and then fitting is performed on measurement data under the wave lengths by the utilization of the least square method according to the kinetic equation of cell delayed luminescence. Kinetic equation parameters obtained in fitting comprise parameters ISL for describing spontaneous luminescence of the sample, and the parameters are the spontaneous luminescence intensity under the wave lengths. Through the method, the spontaneous photon radiation spectrum of the sample can be measured indirectly. The problem that due to the fact that the spontaneous photon radiation intensity of the sample is too low, the spectrum of the sample cannot be detected directly is avoided.
Description
technical field [0001] The invention belongs to the technical field of biological ultra-weak photon radiation, and in particular relates to a method for detecting the spectrum of biological ultra-weak photon radiation. Background technique [0002] Biological ultra-weak photon radiation, also known as biological ultra-weak luminescence, includes spontaneous luminescence (also known as self-photon radiation) and delayed luminescence induced by external light (also known as photoluminescence). Weak quasi-continuous photon radiation with a luminous intensity of about a few hundred hv / s cm 2 , the wavelength range is about 180-800nm. Among the various life information emitted by crops, light information is the life information with the largest amount of information and the easiest to achieve non-destructive measurement, so the study of it is of great significance. [0003] At present, most researches on biological ultra-weak photon radiation (including spontaneous luminescence...
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IPC IPC(8): G01J3/443
Inventor 刘锴习岗樊琳琳赵燕燕
Owner XIAN UNIV OF TECH
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