Restoration method of lipase activity
A recovery method and activity recovery technology, applied in the directions of hydrolase, fermentation, etc., can solve the problems of reduced lipase activity, insufficient activity recovery, uneven recovery of lipase activity, etc., and achieve the effect of improving activity
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Embodiment 1
[0117] 10 g of lipase cake A (moisture content relative to the mass of components other than oily components contained in the lipase-containing composition: 8% by mass) was spread on a glass dish, and kept at a temperature of 60°C and a relative humidity of 70%. Keep for 1 day under an atmosphere of humidified gas (humid air). The amount of water absorbed by the retained lipase cake is 10% by mass relative to the mass of the lipase cake A (the amount of moisture relative to the mass of the components other than the oily components contained in the lipase-containing composition is 18% by mass), the amount of oily components in the lipase-containing composition did not change. Then, using the obtained lipase cake, the above-mentioned transesterification reaction was performed once. In addition, in order to investigate the recovery of lipase activity or the degree of activity (POS+SOS value), transesterification was carried out in the same manner as above using the lipase cake A...
Embodiment 2
[0126] Spread 10g of lipase filter cake A on a glass dish and keep it under the atmosphere of moist gas (humid air) with relative humidity of 50%, 70% and 90% at 30°C, 40°C, 50°C and 60°C. 1 hour or 1 day. Table 3 shows the water content (% by mass) relative to the mass of the components other than the oily components contained in the lipase-containing composition. Then, the lipase filter cake was recovered, and the above-mentioned transesterification reaction was performed once. The total mass % of POS and SOS in the triglyceride obtained after carrying out the transesterification reaction of the lipase cake A before using the moisture absorption retention at this time for 1 hour was set to 100. In this case, when using the moisture absorption retention Table 4 shows the relative amount (POS+SOS value) of the total mass % of POS and SOS in the triglyceride obtained after the transesterification reaction of the lipase cake was carried out for 1 hour.
[0127] table 3
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Embodiment 3
[0135]Add 4 g of lipase filter cake A to 400 g of the aforementioned reaction matrix (ethyl stearate: high oleic sunflower oil = 6:4 (mass ratio)), and react at 50° C. for 18 hours. After the reaction, use a pressure filter (Advantec KST-142-JA) to carry out pressure filtration with dry nitrogen (pressure condition: 0.1 MPa) to obtain a post-reaction filter cake (comparative filter cake). Furthermore, the nitrogen gas was ventilated and dried (up to 5 minutes), and the oily components in the filter cake after the reaction were removed to obtain the post-ventilated filter cakes of Trials 3-1 to 3-6. Based on the multiple of the mass of the oily component contained in the filter cake (lipase-containing composition) after aeration relative to the mass of the components in the filter cake other than the oily component, the amount of oily component in the aerated filter cake is measured . In addition, the water content of the aerated cake (lipase-containing composition) was measur...
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