Animal lyophilized inactivated vaccine and preparation process thereof
A technology for inactivated vaccines and animals, which can be used in freeze-dried transportation, medical preparations containing active ingredients, drug combinations, etc. It can solve the problems of affecting product quality and immune effect, cumbersome emulsification process, and single adjuvant dosage form. Achieve the effect of solving the difficulty in satisfying the antigen content of the combined vaccine, solving the problem of excessive immunization times, and good emulsifying performance
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Embodiment 1
[0028] Preparation of freeze-dried inactivated vaccine against Streptococcus suis
[0029] 1. Antigen preparation Inoculate 5-15% of Streptococcus suis production seeds into buffered modified Martin broth with a pH value of 7.2-7.6. The temperature was 37°C, the culture was carried out with low-speed agitation and without steam. At the beginning of the cultivation, 50% glucose solution was added at a ratio of 0.2%, and 2-5% bovine serum was added for feeding. After culturing for 4-6 hours, add an appropriate amount of buffered modified Martin broth containing 0.2% glucose and 2-5% bovine serum, stop feeding 4 hours before the end of the culture, and the final glucose content does not exceed 0.2%. After culturing for 12-18 hours, harvest the culture fluid to obtain the vaccine antigen bacteria fluid.
[0030] 2. Inactivation Add formaldehyde solution to the antigenic bacteria solution that has passed the antigen content, sterility test, and safety test to make the final conce...
Embodiment 2
[0033] Preparation of porcine circovirus type 2 freeze-dried inactivated vaccine
[0034] 1. Antigen preparation Porcine circovirus type 2 (PCV-2) was inoculated on well-growing PK15 cells, cultured in suspension at 37°C, cultured for 24-72 hours, and the cell culture medium was harvested.
[0035] 2. Inactivation: Add an appropriate inactivator to the cell culture medium that has passed the antigen content and sterility test to make the final concentration 0.1-0.5%, inactivate at 37°C for 48 hours, harvest the antigen solution and put it in a -20°C freezer Save it for later use.
[0036] 3. Freeze-drying. Make the sterile inactivated antigen solution into a homogenate with a 1:1-5 ratio of freeze-drying protective agent, add an appropriate amount of antibiotics, mix thoroughly, and quickly follow the appropriate freeze-drying process curve after quantitative packaging. Freeze and vacuum dry to prepare the finished product of freeze-dried inactivated vaccine.
Embodiment 3
[0038] Preparation of Freeze-dried Inactivated Vaccine of Porcine Pseudorabies
[0039] 1. Antigen preparation Inoculate porcine pseudorabies virus on well-grown monolayer chicken embryo fibroblasts, culture in rotation at 37°C, observe the cell lesion every day, and harvest the cell culture medium when the cell lesion is more than 75%.
[0040] 2. Inactivation: Add an appropriate inactivator to the cell culture medium that has passed the antigen content and sterility test so that the final concentration is 0.1-1%, inactivate at 25-37°C for 24-48 hours, harvest the antigen solution and put it in Store in -20°C freezer for later use.
[0041] 3. Freeze-drying. Make the sterile inactivated antigen solution into a homogenate with a 1:1-5 ratio of freeze-drying protective agent, add an appropriate amount of antibiotics, mix thoroughly, and quickly carry out freeze-vacuum drying after quantitative packaging to prepare a freeze-dried Dry inactivated vaccine products.
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