Dust suppressing agent prepared on basis of microorganisms and use method thereof
A dust suppressant and microbial technology, which is applied in the field of microbiology and environmental protection, can solve the problems of complex processing and spraying process, short time of watering and dust suppression, poor environmental compatibility, etc., and achieve good dust consolidation effect and dust suppression effect Obvious effect of improving wind erosion resistance
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Embodiment 1
[0032] (1) Weigh 13g of sucrose, Na 2 HPO 4 12H 2 O4g, MgSO 4 0.7g, CaCO 3 1.5g, KCl0.3g, (NH 4 ) 2 SO 4 Dissolve 0.6g and 0.5g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and let it cool down, and inoculate the wet cells of Bacillus colioids Put into the cooling medium solution, shake culture at 30°C, the shaking frequency is 170r / min, and the culture time is 24h;
[0033] (2) Choose a soluble calcium salt solution with a calcium ion concentration of 1.0mol / L;
[0034](3) Uniformly mix the Bacillus coliformum solution and the soluble calcium salt solution to make a microbial dust suppressant, the ratio of the Bacillus coliformum solution and the soluble calcium salt solution is 150mL:100mL;
[0035] (4) Take the collected dust and put it on an iron plate and spread it evenly. The accumulated volume of dust is 800cm 3 , and vibrate evenly after laying flat; take mi...
Embodiment 2
[0039] (1) Weigh 7g of sucrose, Na 2 HPO 4 12H 2 O1g, MgSO 4 0.3g, CaCO 3 0.5g, KCl0.1g, (NH 4 ) 2 SO 4 Dissolve 0.2g and 0.1g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and let it cool, and inoculate the wet cells of Bacillus colioids Put into the cooling medium solution, shake culture at 30°C, the shaking frequency is 170r / min, and the culture time is 24h;
[0040] (2) Choose a soluble calcium salt solution with a calcium ion concentration of 0.1mol / L;
[0041] (3) Uniformly mix the Bacillus coliformum solution and the soluble calcium salt solution to make a microbial dust suppressant, the ratio of the Bacillus coliformum solution and the soluble calcium salt solution is 150mL:300mL;
[0042] (4) Take the collected dust and put it on an iron plate and spread it evenly. The accumulated volume of dust is 600cm 3 , and vibrate evenly after paving; take microbial du...
Embodiment 3
[0046] (1) Weigh 10g of sucrose, Na 2 HPO 4 12H 2 O2.5g, MgSO 4 0.5g, CaCO 3 1.0g, KCl0.2g, (NH 4 ) 2 SO 4 Dissolve 0.25g and 0.2g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and let it cool, and inoculate the wet cells of Bacillus colioids Put into the cooling medium solution, shake culture at 30°C, the shaking frequency is 170r / min, and the culture time is 24h;
[0047] (2) Choose a soluble calcium salt solution with a calcium ion concentration of 0.5mol / L;
[0048] (3) Uniformly mix the Bacillus colloidus solution and the soluble calcium salt solution to make a microbial dust suppressant, and the ratio of the Bacillus coliform solution and the soluble calcium salt solution is 150mL: 150mL;
[0049] (4) Take the collected dust and put it on an iron plate and spread it evenly. The accumulated volume of dust is 700cm 3 , and vibrate evenly after paving; take microb...
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