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Method and reagent for improving hematopoietic stem cell homing and implanting rate

A technology of hematopoietic stem cells and engraftment rate, applied in the fields of biomedicine and hematopoietic stem cell transplantation, can solve the problems of homing and engraftment efficiency of hematopoietic stem cells that have not yet been found, and achieve the goal of increasing homing and engraftment efficiency, improving transplantation success rate, The effect of shortening the time

Active Publication Date: 2014-05-14
杭州湃莱生物科技有限公司
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Problems solved by technology

Both hLF1–11 and OP-145 have entered phase II clinical trials, and their safety has been verified, but no one has been found to use hLF1–11 and OP-145 to pretreat hematopoietic stem cells in vitro to improve their homing and engraftment efficiency

Method used

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  • Method and reagent for improving hematopoietic stem cell homing and implanting rate
  • Method and reagent for improving hematopoietic stem cell homing and implanting rate
  • Method and reagent for improving hematopoietic stem cell homing and implanting rate

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Embodiment Construction

[0023] 1. Obtain human umbilical cord blood mononuclear cells: the umbilical cord blood provided by healthy donors is mixed with phosphate buffered saline and then centrifuged. The obtained cells are mixed with red blood cell lysate (Pharm Lyse buffer, BD Biosciences). Rinse with salt buffer solution, centrifuge to obtain human umbilical cord blood mononuclear cells containing hematopoietic stem cells, resuspend in appropriate solution, and set aside.

[0024]2. Detection of the effect of cationic peptide derivatives on the differentiation function of human umbilical cord blood hematopoietic stem cells: the human umbilical cord blood mononuclear cells obtained by the above method were resuspended in RPMI1640 medium (Hyclone) containing 0.5% bovine serum albumin and 20 ug / ml hLF1–11 (Peptisyntha) or / and 2 ug / ml OP145 (OctoPlus Technologies and Leiden University Medical Center) at 37°C, 5% CO 2 After incubation for 1 hour under conditioned conditions, the cells were mixed with ...

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Abstract

The invention provides a method and reagent for improving the hematopoietic stem cell homing and implanting rate. The method comprises the following steps that after incubation of donor cells containing hematopoietic stem cells and positive ion derivatives, the cells are transplanted to a body of a receptor, and therefore the effects that the hematopoietic stem cell homing and implantation are enhanced, receptor hematopoietic reconstitution time is shortened, and the success rate of hematopoietic stem cell implantation is improved are achieved. The invention also provides application of the positive ion derivatives in preparation of medicine for treating diseases of the blood system.

Description

[0001] technical field [0002] The invention belongs to the technical field of biomedicine, in particular to the field of hematopoietic stem cell transplantation, and more specifically relates to a method and a reagent for improving the homing and implantation rate of hematopoietic stem cells. Background technique [0003] Since the discovery in the 1950s that bone marrow transplantation can treat lethally irradiated mice, the hematopoietic reconstruction function of hematopoietic stem cells has received widespread attention. Hematopoietic stem cells are a class of cells capable of self-replication and differentiation into mature whole blood cells. In adults, hematopoietic stem cells exist in specific microenvironments—niches. The microenvironment supports hematopoietic stem cells to maintain their normal biological functions, including self-replication, differentiation, migration, etc., through soluble and membrane surface factors. In adults, the bone marrow is considere...

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Application Information

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IPC IPC(8): A61K38/16A61K38/10A61K35/50A61K35/28A61K35/14A61K47/42A61P35/02A61K35/51
Inventor 吴皖汤威罗琳邢文彦
Owner 杭州湃莱生物科技有限公司
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