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Method of improving homing and engraftment rate of hematopoietic stem cells and reagent of method

A technology of hematopoietic stem cells and implantation rate, applied in the field of hematopoietic stem cell transplantation and biomedicine, to increase the efficiency of homing and implantation, make up for the shortage of sources, and improve the success rate of transplantation

Active Publication Date: 2018-08-17
BEIJING DAXI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method of improving homing and engraftment rate of hematopoietic stem cells and reagent of method
  • Method of improving homing and engraftment rate of hematopoietic stem cells and reagent of method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Detection of the effect of small molecule peptides on the differentiation function of human umbilical cord blood hematopoietic stem cells

[0030] Obtain human umbilical cord blood mononuclear cells: the umbilical cord blood provided by healthy donors is mixed with phosphate buffered saline and centrifuged, and the obtained cells are mixed with red blood cell lysate (Pharm Lyse buffer, BD Bisciences), after red blood cells are removed, they are treated with phosphate buffered saline Wash with liquid and centrifuge to obtain human umbilical cord blood mononuclear cells containing hematopoietic stem cells, resuspend in appropriate solution, and set aside.

[0031] Detect the effect of small molecule peptides on the differentiation function of human umbilical cord blood hematopoietic stem cells: the human umbilical cord blood mononuclear cells obtained by the above method were resuspended in RPMI1640 medium (Hyclone) containing 0.5% bovine serum albumin and 2ug / ml...

Embodiment 2

[0032] Example 2 Measuring the effect of small molecular peptide pretreatment on the homing and implantation efficiency of human umbilical cord blood hematopoietic stem cells and the time of hematopoietic reconstitution

[0033]Human umbilical cord blood hematopoietic stem cells pretreated with small molecular peptides (2ug / ml hLF5-29 or / and 4ug / ml OP330, 2g / ml OP145 as a control, incubated for 30 minutes under the conditions), were injected into the recipient γ Radiation irradiated (450cGY) severe combined immunodeficiency mice (NOD-SCID mice, 6-, male) in vivo. On the 11th day after transplantation, the mouse femur was collected for mononuclear cell count and colony forming unit test to detect the effect of pretreatment on the homing of hematopoietic stem cells. Such as figure 1 The results showed that the homing efficiency of human umbilical cord blood hematopoietic stem cells pretreated with small molecule peptides was significantly increased, and the number of monocytes ...

Embodiment 3

[0036] Example 3 Security Testing

[0037] 1. Experimental drugs and experimental animals

[0038] Experimental medication: take the two kinds of polypeptides provided by the present invention, and prepare oral liquids with a concentration of 2 mg / ml with physiological saline, respectively, for intragastric administration in experiments. Experimental animals: the animals in this experiment are all BABC mice, 10 in each group, divided into random groups. A total of 40 weeks was carried out, and the experimental animals were observed after administration. No adverse drug reactions, no side effects, no toxicity, carcinogenicity, teratogenicity, and allergenic reactions were found, proving the safety of the two polypeptides for medicinal use.

[0039] In conclusion, from the above results, the two polypeptides provided by the present invention have significant progress in improving the homing function of hematopoietic stem cells.

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Abstract

The invention relates to a method of improving the homing and engraftment rate of hematopoietic stem cells and a reagent of the method. By taking small molecule peptides as a main component to performpretreatment on the hematopoietic stem cells, the method provided by the invention improves the homing and engraftment rate of the hematopoietic stem cells in vivo, shortens the time required for reconstruction of a hematopoietic system, improves the success rate of hematopoietic stem cell transplantation and makes up for the status quo of insufficient sources of the hematopoietic stem cells. Inaddition, low-dose intravenous infusion of the small molecule peptides does not have bad influence on the safety of a human body. Thus, the invention actually provides application of the small molecule peptides in preparing a drug for treating diseases of a blood system.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to the field of hematopoietic stem cell transplantation, and more specifically relates to a method for improving the homing and implantation rate of hematopoietic stem cells and a reagent thereof. Background technique [0002] Hematopoietic stem cells refer to a class of cells with self-renewal and multidirectional differentiation capabilities. Its basic feature is self-renewal ability, that is, after a cell cycle activity, it can produce two hematopoietic stem cells with the same properties as before division, and it also has multi-directional differentiation ability, that is, under certain environmental conditions, hematopoietic stem cells have The ability to differentiate into various blood cell lines. [0003] Hematopoietic stem cell transplantation is currently widely used in the treatment of malignant blood diseases, non-malignant refractory blood diseases, genetic diseas...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P7/00A61P37/02
CPCA61K38/16A61P7/00A61P37/02
Inventor 杨骏张勇
Owner BEIJING DAXI BIOTECHNOLOGY CO LTD
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