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Pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and application thereof

A technology of Pseudomonas stutzeri and nitrifying bacteria, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc.

Active Publication Date: 2014-05-14
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the secondary effluent of urban sewage contains a certain amount of nutrients such as nitrogen and phosphoru

Method used

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  • Pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and application thereof
  • Pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and application thereof
  • Pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Isolation and identification of Pseudomonas stutzeri DN-LWX19

[0040] 1. Isolation of Pseudomonas stutzeri DN-LWX19

[0041] Place 10 filter materials at the outlet of the denitrification filter (see Table 1 for the water quality on the day of sampling) into a 40mL enriched medium (use 5g beef extract, 10g peptone, 1g potassium nitrate and water to make 1 liter of liquid, pH Incubate overnight at 30°C in a 100mL Erlenmeyer flask of 7.0-7.2) to allow the microorganisms on the filter material to be dispersed and enriched and grow, and pipette 1mL of bacterial suspension into a centrifuge tube containing 9mL of sterile water to obtain a dilution of 10 -1 The bacterial suspension, according to this method, dilute the bacterial suspension with sterile water to 10 -15 , To obtain a series of dilution gradient bacterial suspension. Dilute 0.1mL separately 10 -8 -10 -15 Bacteria suspension to denitrifying bacteria isolation medium (use beef extract 5g, peptone 10g, potas...

Embodiment 2

[0067] Example 2. Preparation of denitrifying bacteria

[0068] The slant medium (each liter of the slant medium is made up of 1 liter of liquid with 5g beef extract, 10g peptone, 1g potassium nitrate, 18g agar and water; pH is 7.0-7.2; sterilized at 121°C for 20 minutes) The preserved Pseudomonas stutzeri (Pseudomonas stutzeri) DN-LWX19CGMCC No.8521 is inoculated with a sterile inoculation loop to pick up a loop to contain 40mL enrichment medium (each liter of enrichment medium is 5g beef extract, 10g of peptone, 1g of potassium nitrate, 0.4g of potassium dihydrogen phosphate and water to make 1 liter of liquid, pH 7.0-7.2; sterilize at 121 ℃ for 20 minutes) in a 100 mL conical flask, shake culture at 30 ℃ 12h, the obtained bacterial solution is the denitrifying bacteria agent, and the content of Pseudomonas stutzeri (Pseudomonas stutzeri) DN-LWX19CGMCC No.8521 in the denitrifying bacteria agent is 2×10 10 cfu / mL.

Embodiment 3

[0069] Example 3. Denitrification effect of Pseudomonas stutzeri DN-LWX19CGMCC No.8521 in simulated sewage

[0070] The sewage is taken from the effluent of the SBR reactor (NO 3 - -N32mg / L, NO 2 - -N4.5mg / L, COD50mg / L), the carbon to nitrogen ratio is about 1.56:1. In order to meet the carbon-nitrogen ratio required for denitrification, sodium acetate was added to the sewage until the COD concentration was 275 mg / L, to obtain simulated sewage. NO of the simulated sewage 3 - -N concentration is 32mg / L, NO 2 - -N concentration is 4.5mg / L, COD concentration is 275mg / L.

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Abstract

The invention discloses pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and an application thereof. The pseudomonas stutzeri is pseudomonas stutzeri DN-LWX19; and a preservation number of the pseudomonas stutzeri in General Microbiology Centre of Microbial Preservation Management Committee in China is CGMCC No.8521. Through the pseudomonas stutzeri DN-LWX19, a NO3<-1>-N removal rate in 24 hours in simulation sewage with a NO3<-1>-N concentration of 32mg/L can reach 98%, the NO3<-1>-N removal rate in 32 hours can reach 100% and no nitrite is accumulated; and a consumption ratio of carbon to nitrogen after achieving complete denitrification in 32 hours is 4.4:1.

Description

Technical field [0001] The invention relates to a strain of Pseudomonas stutzeri for efficiently treating nitrogenous sewage and its application. Background technique [0002] Sewage recycling is an effective way to alleviate water shortages, prevent and control water environmental pollution, and improve water environmental quality. However, since the secondary effluent of urban sewage contains a certain amount of nutrients such as nitrogen and phosphorus, which directly replenish urban landscape water bodies, rivers and lakes, there is a risk of eutrophication of water bodies. At present, biological nitrogen removal technology is still the most efficient and widely used. [0003] The conversion process of nitrogen in traditional biological denitrification includes ammoniation, nitrification and denitrification. The denitrification process is the process of converting nitrogen into gaseous nitrogen and finally removing it from the water body. Denitrification is achieved through ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 孙迎雪廉巍雪周晓黎田媛
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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