Preparation of semiquantitative and quick B-class trichothecenes toxin detection agent plate

A technology of trichothecenes and reagent plates, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection cost, unfavorable popularization and application, and complicated processing, so as to achieve low detection cost, good specificity, The effect of high sensitivity

Inactive Publication Date: 2014-05-14
JIANGSU WISE SCI & TECH DEV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the detection methods of class B trichothecenes include high performance liquid chromatography (HPLC), gas chromatography (GC), thin layer chromatography (TLC), etc., because the sample pretreatment of the above analytical methods is relatively complicated , special technicians are required for operation, and the detection cost is expensive, which is not conducive to popularization and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of semiquantitative and quick B-class trichothecenes toxin detection agent plate
  • Preparation of semiquantitative and quick B-class trichothecenes toxin detection agent plate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Detection of class B trichothecenes in wheat:

[0024] 1) Pre-processing:

[0025] Accurately weigh 5 g of uniformly pulverized samples into a conical flask, add 25 mL of 10% methanol aqueous solution as the extract, shake for 10 min, filter quickly with qualitative filter paper, collect the filtrate, and take the filtrate for analysis with a reagent plate;

[0026] 2) How to use the semi-quantitative rapid test kit for type B trichothecenes:

[0027] Take 100 μL of the sample filtrate and slowly drop it on the glass fiber membrane of the reagent plate, and observe the color development of the detection line (T line) and the control line (C line) on the reagent plate after 15 min. Determine the content of Class B trichothecenes in the sample extract according to the detection principle of the reagent board: if only one red color band appears, the sample solution is judged to be positive (greater than 1 μg / g), and Class B single-ended The trichothecenes of c...

Embodiment 2

[0028] Example 2 Detection of Class B trichothecenes in beer

[0029] 1) Pre-processing:

[0030] Take a 10 mL sample of beer and stir to remove excess CO 2 , until no obvious bubbles are produced, and then use the reagent plate to detect and analyze after standing still;

[0031] 2) How to use the semi-quantitative rapid test kit for type B trichothecenes:

[0032]Take 100 μL of the sample filtrate and slowly drop it on the glass fiber membrane of the reagent plate, and observe the color development of the detection line (T line) and the control line (C line) on the reagent plate after 15 minutes. Determine the content of Class B trichothecenes in the sample extract according to the detection principle of the reagent board: if only one red color band appears, the sample solution is judged to be positive (greater than 1 μg / g), and Class B single-ended The trichothecenes of class B exceed the standard; if two red color bands appear, it is judged that the sample solution is ...

Embodiment 3

[0033] Example 3 Detection of Class B trichothecenes in feed

[0034] 1) Pre-processing:

[0035] Crush the sample with a pulverizer, store at room temperature, weigh 20 g of the crushed sample, place it in a 200 mL conical flask with a stopper, add 8 mL of water and 100 mL of chloroform-dehydrated ethanol, and chloroform-dehydrated ethanol The volume ratio is 8:2, seal tightly, coat the bottle stopper with water, cover tightly to prevent leakage, shake for 1 hour, filter through folded rapid qualitative filter paper, take 25 mL of filtrate in a 75 mL glass evaporating dish, and place at 90 °C Ventilate and evaporate on a water bath, dissolve the residue in the evaporating dish with 50 mL of petroleum ether in stages, wash it into a 100 mL separating funnel, and then wash the evaporating dish with 20 mL of methanol water, methanol:water volume 8:2 , into the same separatory funnel, shake the separatory funnel for 1.5 min, let it stand for 15 min to separate the layers, and ta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses preparation and use methods for a semiquantitative and quick B-class trichothecenes toxin detection agent strip and belongs to the technical field of food safety detection. The invention relates to a method for preparing the semiquantitative and quick B-class trichothecenes toxin detection agent strip by applying a toxin antibody capable of specifically identifying B-class trichothecenes and a use method of the semiquantitative and quick B-class trichothecenes toxin detection agent strip and aims at widening the application prospect of quick detection on the B-class trichothecenes toxin in food cereals in China. Due to the research on a novel immune quick detection product, the difficulty that the detection is affected by a fact that a toxin standard substance source is difficult to find is solved, and the detection cost is reduced; safety detection conditions are supplied to a detector; the semiquantitative and quick B-class trichothecenes toxin detection agent strip has the advantages of simplicity, quickness, high sensitivity, high specificity, safety, environment friendliness and the like.

Description

technical field [0001] The invention discloses a method for preparing and using a type B trichothecene toxin semi-quantitative rapid test reagent board, which belongs to the technical field of biological and food safety detection. Background technique [0002] Class B trichothecenes are a class of metabolites produced by Fusarium fungi, which are widely distributed in nature, mostly in wheat, barley, corn and other grain seeds, and are the most dangerous food that occurs naturally Pollutants are very harmful to humans and animals. It not only causes acute and chronic poisoning of humans and animals, but also has potential risks of carcinogenicity, teratogenicity and mutagenesis. Class B trichothecenes mainly include deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3-acetyl-DON, 3-Ac-DON), 15- Acetyl deoxynivalenol (15-acetyl-DON, 15-Ac-DON), nivalenol (nivalenol, NIV) and fusarenon X (FUS-X), etc. [0003] Class B trichothecenes are a global class of cereal contaminants fou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 杜道林吴湾湾
Owner JIANGSU WISE SCI & TECH DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap