Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of spirulina polysaccharide

A technology of spirulina polysaccharide and spirulina, which is applied in the field of preparation of spirulina polysaccharide, can solve the problems of large consumption of organic solvents, low sugar extraction rate, long process flow, etc., avoid the use of organic solvents, improve the purity, simple craftsmanship

Active Publication Date: 2016-05-04
FUZHOU UNIV
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods of preparing spirulina polysaccharide reported at home and abroad are mainly boiled, concentrated, and alcohol-precipitated processes. The sugar extraction rate is low and time-consuming, and a large amount of organic solvent ethanol is used in the separation stage to remove protein. The consumption of organic solvents is large and the process is long. , large loss of sugar

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 100g of spirulina algae powder, use pH=8.0 NaOH aqueous solution as the extraction solution, stir and extract for 2 hours (90°C water bath, solid-to-liquid ratio 1:15), centrifuge to take the supernatant, adjust the pH value to 4.0, and centrifuge again to take the supernatant The liquid is the upper column liquid. Flow through a cation exchange resin column 732 (10×100 cm) at a flow rate of 1 bed volume / hour to collect polysaccharide components, use a 10kDa ultrafiltration membrane for concentration and desalination, and then spray dry to obtain high-purity polysaccharide powder. The product is pale yellow with a purity of 96.3%.

Embodiment 2

[0020] Take 10 g of low-purity spirulina polysaccharide (purity 30.2%), use water as the extraction solution, stir and dissolve for 10 min (60°C water bath, solid-to-liquid ratio 1:30), adjust the pH value to 4.0, and centrifuge to remove the precipitate. Adjust the pH to 10, and centrifuge again to get the supernatant, which is the upper column liquid. Flow through anion exchange resin D254 (20×100cm) at a flow rate of 1 bed volume / hour, and use 2% sodium chloride solution for dynamic elution. It is the upper column liquid. The polysaccharide fraction was collected, concentrated and desalted using a 10kDa ultrafiltration membrane, and then freeze-dried to obtain a high-purity polysaccharide powder. The product is white with a purity of 98.5%.

Embodiment 3

[0022] Take 100g of fresh spirulina, use pH=9.0NaOH aqueous solution as the extraction solution, stir and extract for 2h (100℃ water bath, solid-to-liquid ratio 1:10), centrifuge to get the supernatant, adjust the pH value to 3.0, centrifuge again to get the supernatant The liquid is the upper column liquid. Flow through a cation exchange resin column D113 (10×100 cm) at a flow rate of 1 bed volume / hour to collect polysaccharide fractions, use a 10kDa ultrafiltration membrane for concentration and desalination, and then freeze-dry to obtain high-purity polysaccharide powder. The product is white with a purity of 97.1%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of spirulina polysaccharide, which uses spirulina as raw material, adopts aqueous solution extraction, isoelectric point method to remove protein, ion exchange resin separation and membrane separation technology to prepare spirulina polysaccharide with a purity greater than 95%. It can be used in the preparation of food, functional food and medicine. Compared with the traditional poaching, concentration and alcohol precipitation process, the present invention has simple process and significantly improved purity; and avoids the use of organic solvents and energy consumption of poaching and concentration, greatly reduces the cost and has great popularization.

Description

technical field [0001] The invention belongs to the technical field of biochemical products, and in particular relates to a preparation method of spirulina polysaccharide. Background technique [0002] Spirulina (Spirulinaplatensis) belongs to Cyanophyta, Oscillatoriaceae, Spirulina genus, and is a filamentous multicellular spiral algal organism. Spirulina has the characteristics of high protein, low fat, and low sugar. It is rich in protein, with a protein content of 60%-70%. It also contains a large amount of various amino acids and biologically active substances necessary for the human body, such as β-carotene, Chlorophyll, α, γ-linolenic acid, vitamins, trace elements, phycocyanin, especially spirulina polysaccharide with special physiological effects. At present, it is believed that spirulina has high utilization value in medical care, and has a variety of physiological functions. It can improve the immune function of the body, promote human metabolism, anti-cancer, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 黄剑东郑永权邱明万东华
Owner FUZHOU UNIV